Density Management in the 21 st Century: West Side Story PNW-GTR-880 Riparian Buffers and Thinning in Headwater Drainages in Western Oregon: Aquatic Vertebrates and Habitats

Abstract Th e Density Management and Riparian Buff er Study (DMS) of western Oregon is a template for numerous research projects on managed federal forestlands. Herein, I review the origins of Riparian Buff er Study component and summarize key fi ndings of a suite of associated aquatic vertebrate projects. Aquatic vertebrate study objectives include characterization of headwater fauna and habitats, and examination of the eff ects on headwater-dwelling species of combined buff er-andthinning treatments in years 1, 2, 5, and 10 post-treatment. Some treatment eff ects have emerged, with negative eff ects on bank amphibian counts occurring in treatments with the narrowest buff ers 10 years post-thinning. Nevertheless, all taxa appear to be persisting at sites. Instream amphibians, in particular, appear to be highly resilient to the types of disturbances resulting from the thinning and buff er treatments of the DMS

The ecological and behavioral dynamics of breeding in three sympatric anuran amphibians

Breeding ecology and behavior were investigated in a field study of three sympatric anuran amphibians in the Oregon Cascade Mountains: the western toad (Bufo boreas), the Cascades frog (Rana cascadae) and the Pacific treefrog (Hyla regilla). A comprehensive study of the western toad mating system was conducted at three populations during five years (15 explosive-breeding aggregations). Two size-dependent pairing patterns (a large male mating advantage and size assortative mating) occurred variably among aggregations and among breeding days within aggregations. Body size variation and the degree of sexual size dimorphism explained much of the mating pattern variability. Field observations and experiments suggest nonrandom mating resulted from male-male exploitative competition for mates and passive female choice of mates. Density and sex ratio had little affect on pairing behavior. Sporadic mass-predation on breeding toads was associated with pair-separations and may have influenced pairing patterns. Toads showed sex and site differences in multiyear breeding schedules, recapture/survival rates, and yearly and multiyear cumulative mating success. The opportunity for selection on toads was partitioned into 3 selection episodes: adult survival, mating success, and fertility. The largest contributors to selection opportunity were mating success for males, and clutch size (fertility) for females. At some aggregations, the opportunity for sexual selection on males was the lowest recorded for anurans. Temporal and spatial synchrony of breeding occurred with Cascade frogs and Pacific treefrogs. During two years, interspecific interactions resulted in the displacement of treefrogs by Cascade frog males from the warmer areas of the ice-covered pond

Host Identity Matters in the Amphibian-Batrachochytrium dendrobatidis System: Fine-Scale Patterns of Variation in Responses to a Multi-Host Pathogen

Species composition within ecological assemblages can drive disease dynamics including pathogen invasion, spread, and persistence. In multi-host pathogen systems, interspecific variation in responses to infection creates important context dependency when predicting the outcome of disease. Here, we examine the responses of three sympatric host species to a single fungal pathogen, Batrachochytrium dendrobatidis, which is associated with worldwide amphibian population declines and extinctions. Using an experimental approach, we show that amphibian species from three different genera display significant differences in patterns of pathgen-induced mortality as well as the magnitude and temporal dynamics of infection load. We exposed amphibians to one of four inoculation dose treatments at both larval and post- metamorphic stages and quantified infection load on day 8 and day 15 post-inoculation. Of the three species examined, only one (the Pacific treefrog; Pseudacris regilla) displayed "dose-dependent" responses; survival was reduced and infection load was elevated as inoculation dose was increased. We observed a reduction in survival but no differences in infection load across pathogen treatments in Cascades frogs (Rana cascadae). Western toads (Anaxyrus boreas) displayed differences in infection load but no differences in survival across pathogen treatments. Within species, responses to the pathogen varied with life history stage, and the most heavily infected species at the larval stage was different from the most heavily infected species at the post-metamorphic stage. Temporal changes in infection load were species and life history stage-specific. We show that variation in susceptibility to this multi-host pathogen is complex when viewed at a fine-scale and may be mediated through intrinsic host traits

Management of Riparian Buffers: Upslope Thinning with Downslope Impacts

We examined the potential of using upslope density management to influence growth and drought tolerance of trees in untreated downslope riparian forests. Increment cores from Douglas-fir trees in three mature stands in western Oregon, USA, were collected and measured. Trees responded to an apparent edge effect up to 15 m downslope of thinned areas but not downslope of gaps. Growth responses in riparian trees were not affected by slope or potential solar radiation (as a function of location and topography). In addition, in a retrospective analysis of tree growth and allocation patterns (represented by the ratio of earlywood to latewood) and climate after treatment over a 12-year period, trees in our study area did not appear to be water limited and did not show a strong correlation with regional drought metrics. We hypothesize that vegetation layers in these riparian forest stands responded differentially to additional resources becoming available as a result of thinning, with overstory trees in riparian areas responding downslope of thinned uplands and subdominant canopy layers responding downslope of gaps. Our study demonstrates that managers can affect riparian forests with upland treatments to a limited spatial extent, which may be the only option in areas where direct riparian management is restricted due to concerns for other ecosystem services.Keywords: riparian management, western Oregon, thinning, climate, drought, riparian zon

DNA repair and resistence to UV-B radiation in western spotted frogs

assessed DNA repair and resistance to solar radiation in eggs of members of the western spotted frog complex (Rana pretiosa and R. luteiventris), species whose populations are suffering severe range reductions and declines. Specifically, we measured the activity of photoreactivating enzyme (photolyase) in oocytes of spotted frogs. In some species, photoreactivation is the most important mechanism for repair of UV-damaged DNA. Using field experiments, we also compared the hatching success of spotted frog embr yos at natural oviposition sites at three elevations, where some embr yos were subjected to ambient levels of UV-B radiation and others were shielded from UV-B radiation. Compared with other amphibians, photolyase activities in spotted frogs were relatively high. At all sites, hatching success was unaffected by UV-B. Our data support the interpretation that amphibian embr yos with relatively high levels of photolyase are more resistant to UV-B radiation than those with lower levels of photolyase. At the embr yonic stage, UV-B radiation does not presently seem to be contributing to the population declines of spotted frogs.Peer reviewe

Heterogeneous Occupancy and Density Estimates of the Pathogenic Fungus Batrachochytrium dendrobatidis in Waters of North America

Biodiversity losses are occurring worldwide due to a combination of stressors. For example, by one estimate, 40% of amphibian species are vulnerable to extinction, and disease is one threat to amphibian populations. The emerging infectious disease chytridiomycosis, caused by the aquatic fungus Batrachochytrium dendrobatidis (Bd), is a contributor to amphibian declines worldwide. Bd research has focused on the dynamics of the pathogen in its amphibian hosts, with little emphasis on investigating the dynamics of free-living Bd. Therefore, we investigated patterns of Bd occupancy and density in amphibian habitats using occupancy models, powerful tools for estimating site occupancy and detection probability. Occupancy models have been used to investigate diseases where the focus was on pathogen occurrence in the host. We applied occupancy models to investigate free-living Bd in North American surface waters to determine Bd seasonality, relationships between Bd site occupancy and habitat attributes, and probability of detection from water samples as a function of the number of samples, sample volume, and water quality. We also report on the temporal patterns of Bd density from a 4-year case study of a Bd-positive wetland. We provide evidence that Bd occurs in the environment year-round. Bd exhibited temporal and spatial heterogeneity in density, but did not exhibit seasonality in occupancy. Bd was detected in all months, typically at less than 100 zoospores L⁻¹. The highest density observed was ~3 million zoospores L⁻¹. We detected Bd in 47% of sites sampled, but estimated that Bd occupied 61% of sites, highlighting the importance of accounting for imperfect detection. When Bd was present, there was a 95% chance of detecting it with four samples of 600 ml of water or five samples of 60 mL. Our findings provide important baseline information to advance the study of Bd disease ecology, and advance our understanding of amphibian exposure to free-living Bd in aquatic habitats over time

Mapping the Global Emergence of Batrachochytrium dendrobatidis, the Amphibian Chytrid Fungus

The rapid worldwide emergence of the amphibian pathogen Batrachochytrium dendrobatidis (Bd) is having a profound negative impact on biodiversity. However, global research efforts are fragmented and an overarching synthesis of global infection data is lacking. Here, we provide results from a community tool for the compilation of worldwide Bd presence and report on the analyses of data collated over a four-year period. Using this online database, we analysed: 1) spatial and taxonomic patterns of infection, including amphibian families that appear over-and under-infected; 2) relationships between Bd occurrence and declining amphibian species, including associations among Bd occurrence, species richness, and enigmatic population declines; and 3) patterns of environmental correlates with Bd, including climate metrics for all species combined and three families (Hylidae, Bufonidae, Ranidae) separately, at both a global scale and regional (U. S. A.) scale. These associations provide new insights for downscaled hypothesis testing. The pathogen has been detected in 52 of 82 countries in which sampling was reported, and it has been detected in 516 of 1240 (42%) amphibian species. We show that detected Bd infections are related to amphibian biodiversity and locations experiencing rapid enigmatic declines, supporting the hypothesis that greater complexity of amphibian communities increases the likelihood of emergence of infection and transmission of Bd. Using a global model including all sampled species, the odds of Bd detection decreased with increasing temperature range at a site. Further consideration of temperature range, rather than maximum or minimum temperatures, may provide new insights into Bd-host ecology. Whereas caution is necessary when interpreting such a broad global dataset, the use of our pathogen database is helping to inform studies of the epidemiology of Bd, as well as enabling regional, national, and international prioritization of conservation efforts. We provide recommendations for adaptive management to enhance the database utility and relevance

Using decision analysis to support proactive management of emerging infectious wildlife diseases

Despite calls for improved responses to emerging infectious diseases in wildlife, management is seldom considered until a disease has been detected in affected populations. Reactive approaches may limit the potential for control and increase total response costs. An alternative, proactive management framework can identify immediate actions that reduce future impacts even before a disease is detected, and plan subsequent actions that are conditional on disease emergence. We identify four main obstacles to developing proactive management strategies for the newly discovered salamander pathogen Batrachochytrium salamandrivorans (Bsal). Given that uncertainty is a hallmark of wildlife disease management and that associated decisions are often complicated by multiple competing objectives, we advocate using decision analysis to create and evaluate trade-offs between proactive (pre-emergence) and reactive (post-emergence) management options. Policy makers and natural resource agency personnel can apply principles from decision analysis to improve strategies for countering emerging infectious diseases

Engineering the Petawatt Laser into Nova

The engineering process of integrating the Petawatt (10{sup 15} watts) laser system into the existing 30 kJ (UV) Nova laser at Lawrence Livermore National Laboratory (LLNL) is described in detail. The nanosecond-long, chirped Petawatt laser pulse is initially generated in a separate master oscillator room and then injected into one of Nova`s 10 beamlines. There, the pulse is further amplified and enlarged to {approximately}{phi}60 cm, temporally compressed under vacuum to <500 fs using large diameter diffraction gratings, and then finally focused onto targets using a parabolic mirror. The major Petawatt components are physically large which created many significant engineering challenges in design, installation and implementation. These include the diffraction gratings and mirrors, vacuum compressor chamber, target chamber, and parabolic focusing mirror. Other Petawatt system components were also technically challenging and include: an injection beamline, transport spatial filters, laser diagnostics, alignment components, motor controls, interlocks, timing and synchronization systems, support structures, and vacuum systems. The entire Petawatt laser system was designed, fabricated, installed, and activated while the Nova laser continued its normal two-shift operation. This process required careful engineering and detailed planning to prevent experimental downtime and to complete the project on schedule