Colocation and role of polyphosphates and alkaline phosphatase in apatite biomineralization of elasmobranch tesserae

AbstractElasmobranchs (e.g. sharks and rays), like all fishes, grow continuously throughout life. Unlike other vertebrates, their skeletons are primarily cartilaginous, comprising a hyaline cartilage-like core, stiffened by a thin outer array of mineralized, abutting and interconnected tiles called tesserae. Tesserae bear active mineralization fronts at all margins and the tesseral layer is thin enough to section without decalcifying, making this a tractable but largely unexamined system for investigating controlled apatite mineralization, while also offering a potential analog for endochondral ossification. The chemical mechanism for tesserae mineralization has not been described, but has been previously attributed to spherical precursors, and alkaline phosphatase (ALP) activity. Here, we use a variety of techniques to elucidate the involvement of phosphorus-containing precursors in the formation of tesserae at their mineralization fronts. Using Raman spectroscopy, fluorescence microscopy and histological methods, we demonstrate that ALP activity is located with inorganic phosphate polymers (polyP) at the tessera–uncalcified cartilage interface, suggesting a potential mechanism for regulated mineralization: inorganic phosphate (Pi) can be cleaved from polyP by ALP, thus making Pi locally available for apatite biomineralization. The application of exogenous ALP to tissue cross-sections resulted in the disappearance of polyP and the appearance of Pi in uncalcified cartilage adjacent to mineralization fronts. We propose that elasmobranch skeletal cells control apatite biomineralization by biochemically controlling polyP and ALP production, placement and activity. Previous identification of polyP and ALP shown previously in mammalian calcifying cartilage supports the hypothesis that this mechanism may be a general regulating feature in the mineralization of vertebrate skeletons

Solution of large scale nuclear structure problems by wave function factorization

Low-lying shell model states may be approximated accurately by a sum over products of proton and neutron states. The optimal factors are determined by a variational principle and result from the solution of rather low-dimensional eigenvalue problems. Application of this method to sd-shell nuclei, pf-shell nuclei, and to no-core shell model problems shows that very accurate approximations to the exact solutions may be obtained. Their energies, quantum numbers and overlaps with exact eigenstates converge exponentially fast as the number of retained factors is increased.Comment: 12 pages, 12 figures (from 15 eps files) include

Milwaukee Independent Charter Schools Study: Report on One Year of Student Growth

Analyzes results of an evaluation of gains in reading and math scores over one academic year among independent charter school students compared with public school students, by charter school type, student characteristics, and school-switching

Study protocol: addressing evidence and context to facilitate transfer and uptake of consultation recording use in oncology: a knowledge translation implementation study

Background: The time period from diagnosis to the end of treatment is challenging for newly diagnosed cancer patients. Patients have a substantial need for information, decision aids, and psychosocial support. Recordings of initial oncology consultations improve information recall, reduce anxiety, enhance patient satisfaction with communication, and increase patients' perceptions that the essential aspects of their disease and treatment have been addressed during the consultation. Despite the research evidence supporting the provision of consultation recordings, uptake of this intervention into oncology practice has been slow. The primary aim of this project is to conduct an implementation study to explicate the contextual factors, including use of evidence, that facilitate and impede the transfer and uptake of consultation-recording use in a sample of patients newly diagnosed with breast or prostate cancer. Methods: Sixteen oncologists from cancer centres in three Canadian cities will participate in this three-phase study. The preimplementation phase will be used to identify and address those factors that are fundamental to facilitating the smooth adoption and delivery of the intervention during the implementation phase. During the implementation phase, breast and prostate cancer patients will receive a recording of their initial oncology consultation to take home. Patient interviews will be conducted in the days following the consultation to gather feedback on the benefits of the intervention. Patients will complete the Digital Recording Use Semi-Structured Interview (DRUSSI) and be invited to participate in focus groups in which their experiences with the consultation recording will be explored. Oncologists will receive a summary letter detailing the benefits voiced by their patients. The postimplementation phase includes a conceptual framework development meeting and a seven-point dissemination strategy. Discussion: Consultation recording has been used in oncology, family medicine, and other medicine specialties, and despite affirming evidence and probable applications to a large number of diseases and a variety of clinical contexts, clinical adoption of this intervention has been slow. The proposed study findings will advance our conceptual knowledge of the ways to enhance uptake of consultation recordings in oncology

Coherent optical control of spin-spin interaction in doped semiconductors

We provide a theory of laser-induced interaction between spins localized by impurity centers in a semiconductor host. By solving exactly the problem of two localized spins interacting with one itinerant exciton, an analytical expression for the induced spin-spin interaction is given as a function of the spin separation, laser energy, and intensity. We apply the theory to shallow neutral donors (Si) and deep rare-earth magnetic impurities (Yb) in III-V semiconductors. When the photon energy approaches a resonance related to excitons bound to the impurities, the coupling between the localized spins increases, and may change from ferromagnetic to anti-ferromagnetic. This light-controlled spin interaction provides a mechanism for the quantum control of spins in semiconductors for quantum information processing; it suggests the realization of spin systems whose magnetic properties can be controlled by changing the strength and the sign of the spin-spin interaction.Comment: 10 pages, 5 figure

BVI Photometry and the Luminosity Function of the Globular Cluster M92

We present new BVI ground-based photometry and VI space-based photometry for the globular cluster M92 (NGC 6341) and examine luminosity functions in B, V, and I containing over 50,000 stars ranging from the tip of the red giant branch to several magnitudes below the main sequence turn off. Once corrected for completeness, the observed luminosity functions agree very well with theoretical models and do not show stellar excesses in any region of the luminosity function. Using reduced chi squared fitting, the new M92 luminosity function is shown to be an excellent match to the previously published luminosity function for M30. These points combine to establish that the "subgiant excess" found in previously published luminosity functions of Galactic globular clusters are due to deficiencies in the stellar models used at that time. Using up to date stellar models results in good agreement between observations and theory. Several statistical methods are presented to best determine the age of M92. These methods prove to be insensitive to the exact choice of metallicity within the published range. Using [Fe/H]=-2.17 to match recent studies we find an age of 14.2 plus or minus 1.2 Gyr for the cluster.Comment: 22 pages, 13 figures, 3 tables, accepted for publication in A

Randomized Composable Core-sets for Distributed Submodular Maximization

An effective technique for solving optimization problems over massive data sets is to partition the data into smaller pieces, solve the problem on each piece and compute a representative solution from it, and finally obtain a solution inside the union of the representative solutions for all pieces. This technique can be captured via the concept of {\em composable core-sets}, and has been recently applied to solve diversity maximization problems as well as several clustering problems. However, for coverage and submodular maximization problems, impossibility bounds are known for this technique \cite{IMMM14}. In this paper, we focus on efficient construction of a randomized variant of composable core-sets where the above idea is applied on a {\em random clustering} of the data. We employ this technique for the coverage, monotone and non-monotone submodular maximization problems. Our results significantly improve upon the hardness results for non-randomized core-sets, and imply improved results for submodular maximization in a distributed and streaming settings. In summary, we show that a simple greedy algorithm results in a $1/3$-approximate randomized composable core-set for submodular maximization under a cardinality constraint. This is in contrast to a known $O({\log k\over \sqrt{k}})$ impossibility result for (non-randomized) composable core-set. Our result also extends to non-monotone submodular functions, and leads to the first 2-round MapReduce-based constant-factor approximation algorithm with $O(n)$ total communication complexity for either monotone or non-monotone functions. Finally, using an improved analysis technique and a new algorithm $\mathsf{PseudoGreedy}$, we present an improved $0.545$-approximation algorithm for monotone submodular maximization, which is in turn the first MapReduce-based algorithm beating factor $1/2$ in a constant number of rounds

Shell Model Monte Carlo Studies of γ\gamma-Soft Nuclei

We present Shell Model Monte Carlo calculations for nuclei within the full major shell 50-82 for both protons and neutrons. The interaction is determined solely by self-consistency and odd-even mass differences. The methods are illustrated for ${}^{124}$Sn, ${}^{128}$Te and ${}^{124}$Xe. We calculate shape distributions, moments of inertia and pairing correlations as functions of temperature and angular velocity. Our calculations are the first microscopic evidence of $\gamma$-softness of nuclei in this region.Comment: uuencoded postscript of manuscript with three figure