The Landscape Form of the Metropolis

When the city disintegrates into an archipelago of fragments a new role is imposed on the landscape as a carrier of topographical characterizations, cohesion and continuity. Patterns such as transportation corridors, settlement areas and landscape voids can be regarded as latent macro-landscape forms of the metropolitan territory. In the staging of the metropolis these forms need to be embedded in a compositional structure that addresses fragmentation and disorientation, without relapsing into utopian forms of the traditional city that have proven inadequate for the metropolitan condition.The potential basis to inform this structure is the landscape itself: permanent, neutral and ubiquitous. The underlying landscape also contains an annotated catalogue of situations, in which the genius loci is recorded and secured. These latent compositional elements are transformed into landscape architectural ‘narratives’ within the topography of the emerging metropolis. The enlargement and distortion of specific topographies result in a field of new topologies, drawn from the genius loci and from local cultures and customs. The question is not so much if metropolitan form is determined by landscape, but how we can use it to structure and give meaning to dispersed territories. This involves a delicate choreography of macro-landscape forms and the micro-topography of landscape places.

Crowding-out or crowding-in: The dynamics of different revenue streams

An important question in public economics is to what extent changes in government funding lead to changes in private donations. In this chapter we identify and summarize four theoretical perspectives answering this question: the micro-economic, institutional-political, institutional signaling, and organizational perspective. Reviewing the empirical support for each perspective, we find that none of the perspectives sufficiently explains the dispersed empirical evidence for the relationship between government financial support and individual philanthropic donations. We argue that the context in which nonprofit organizations operate is a relevant but often overlooked factor that influences how government support affects philanthropic giving. Research in this area should adopt a dynamic perspective, taking into account the dynamics of different nonprofit revenue streams (from governments, businesses, foundations, households) as well as contextual level factors like the subsector of the nonprofit sector and country characteristics.The work by Pamala Wiepking at the Lilly Family School of Philanthropy is funded through a generous donation by the Stead Family, the work by Arjen de Wit and Pamala Wiepking at the Vrije Universiteit Amsterdam is funded by the Dutch Charity Lotteries

The circumstellar disk, envelope, and bi-polar outflow of the Massive Young Stellar Object W33A

The Young Stellar Object (YSO) W33A is one of the best known examples of a massive star still in the process of forming. Here we present Gemini North ALTAIR/NIFS laser-guide star adaptive-optics assisted K-band integral-field spectroscopy of W33A and its inner reflection nebula. In our data we make the first detections of a rotationally-flattened outer envelope and fast bi-polar jet of a massive YSO at near-infrared wavelengths. The predominant spectral features observed are Br-gamma, H_2, and a combination of emission and absorption from CO gas. We perform a 3-D spectro-astrometric analysis of the line emission, the first study of its kind. We find that the object's Br-gamma emission reveals evidence for a fast bi-polar jet on sub-milliarcsecond scales, which is aligned with the larger-scale outflow. The hybrid CO features can be explained as a combination of hot CO emission arising in a disk close to the central star, while cold CO absorption originates in the cooler outer envelope. Kinematic analysis of these features reveals that both structures are rotating, and consistent with being aligned perpendicularly to both the ionised jet and the large-scale outflow. Assuming Keplerian rotation, we find that the circumstellar disk orbits a central mass of >10Msun, while the outer envelope encloses a mass of ~15Msun. Our results suggest a scenario of a central star accreting material from a circumstellar disk at the centre of a cool extended rotating torus, while driving a fast bi-polar wind. These results therefore provide strong supporting evidence for the hypothesis that the formation mechanism for high-mass stars is qualitatively similar to that of low-mass stars.Comment: 13 pages, 18 figs. Accepted for publication in MNRA

Prorenin accumulation and activation in human endothelial cells: importance of mannose 6-phosphate receptors

ACE inhibitors improve endothelial dysfunction, possibly by blocking endothelial angiotensin production. Prorenin, through its binding and activation by endothelial mannose 6-phosphate (M6P) receptors, may contribute to this production. Here, we investigated this possibility as well as prorenin activation kinetics, the nature of the prorenin-activating enzyme, and M6P receptor-independent prorenin binding. Human umbilical vein endothelial cells (HUVECs) were incubated with wild-type prorenin, K/A-2 prorenin (in which Lys42 is mutated to Ala, thereby preventing cleavage by known proteases), M6P-free prorenin, and nonglycosylated prorenin, with or without M6P, protease inhibitors, or angiotensinogen. HUVECs bound only M6P-containing prorenin (K(d) 0.9+/-0.1 nmol/L, maximum number of binding sites [B(max)] 1010+/-50 receptors/cell). At 37 degrees C, because of M6P receptor recycling, the amount of prorenin internalized via M6P receptors was >25 times B(max). Inside the cells, wild-type and K/A-2 prorenin were proteolytically activated to renin. Renin was subsequently degraded. Protease inhibitors interfered with the latter but not with prorenin activation, thereby indicating that the activating enzyme is different from any of the known prorenin-activating enzymes. Incubation with angiotensinogen did not lead to endothelial angiotensin generation, inasmuch as HUVECs were unable to internalize angiotensinogen. Most likely, therefore, in the absence of angiotensinogen synthesis or endocytosis, M6P receptor-mediated prorenin internalization by endothelial cells represents prorenin clearance