MAMMALIAN CAVE FAUNA FROM THE LATE PLEISTOCENE OF THE WESTERN OZARK PLATEAU, OKLAHOMA

The Ozarks are a highland system riddled with caves across multiple states within the South-Central United States. During the late Pleistocene retreat of the North American Laurentide ice sheet, habitat and species composition of this region was indicative of a more temperate climate. Many studies have ascertained a strong boreal influence on late Pleistocene mammalian faunas in the central Ozarks, but less is known of this period in the highland region of Eastern Oklahoma. This study focuses on a site designated as AD-14 located within the Duncan Field Cave Complex of Adair County, Oklahoma which straddles the border of Oklahoma and Missouri and represents the westernmost edge of the Ozark Plateau. Material was collected from muddy deposits in the cave floor where bones were visibly accumulated as a lag deposit. Preliminary dating yielded a conventional radiocarbon age of 12,304± 50 years for this site. Further radiocarbon analysis is needed to determine if this is a temporally mixed or contemporaneous deposit. Here, we introduce the mammalian fauna found in this late Pleistocene accumulation, which is dominated by small mammalian remains. As the Duncan Cave Complex is currently regarded one of the most biologically rich cave an analysis of species has indicated key differences between current and late Pleistocene environments. Extralocal taxa such as bog lemmings, star-nosed moles, meadow jumping mice, and southern red-backed voles were all cohabiting the Ozark Highland ecoregion during this time, indicative of a cooler climate than modern day and giving insight into future species reactions to anthropogenic climate change

Agate Fossil Beds National Monument, Paleontological Resources Management Plan (Public Version)

Executive Summary Since Agate Springs Ranch was founded by James H. Cook in 1887, exquisite examples of transitional Miocene mammalian fauna have been found along this stretch of the Niobrara River valley. Collectively these paleontological discoveries, along with the existing archeological and historical Native American collection, were the basis for establishing Agate Fossil Beds National Monument (AGFO) as a unit of the National Park System (NPS). The fossil remains from the Harrison and Anderson Ranch formations span a short, but important, time period within the Miocene Epoch. AGFO has provided science with an intimate look into North American mammalian evolution of the time that is matched nowhere else, with body fossils and trace fossils (burrows) of many mammals in excellent condition. Investigation of the paleontological resources at AGFO has been very limited since its establishment, but the opportunities for research and discovery are still substantial. Public and academic interest in the Monument’s paleontological resources are considerable. Although there are existing legal authorities, policies and guidelines regarding the management of paleontological resources, at both the departmental and agency levels, more specific guidance would be helpful for the management of AGFO’s non-renewable fossils. This document has been prepared to provide more specific guidance and recommendations for paleontological resources management at AGFO. The Introduction outlines the significance of AGFO’s paleontological resources and defines the purpose, need, and objectives for the Agate Fossil Beds National Monument Paleontological Resources Management Plan (PRMP). This plan also identifies the legal authorities, requirements, and mandates underpinning AGFO’s mission as a unit of the NPS, with special attention to authorities that address managing and preserving paleontological resources. Background Geology and Paleontology provides a basic park geologic description, discusses the scope of AGFO’s paleontological resources, and summarizes past paleontological work performed at the Monument. This information includes historical information from periods both before and after authorization of the Monument as a unit of the NPS. This section also presents the paleontological significance of AGFO and its specimens, such as how AGFO’s taxa are cornerstones of North American geochronology and biostratigraphy. Paleontological Resources Management begins by listing in greater detail the strategic objectives related to paleontological resources within the NPS and at AGFO. This section then proceeds to discuss the specific considerations related to paleontological resource inventories and monitoring along with management requirements (from policy and guidelines) specific to AGFO. This section discusses what a paleontology inventory is and why, when and how to conduct one; fundamentals of paleontological resource monitoring; the various types of threats to paleontological resources and how to mitigate them; and resource condition assessment and site monitoring protocols. It also addresses how to handle paleontological resources discovered or recovered during other park activities. Paleontological Research Management presents NPS and AGFO research goals, how to evaluate the scientific significance of research, and how to weigh the significance of research against other park mandates, operations, and goals. The section also includes a description of the permitting process, recommended park-specific permit conditions, and rules for overseeing collection and excavation. Museum Collections and Curation documents AGFO’s current paleontological collections, collections management and curation policies, AGFO’s photographic archives, collections from AGFO in external repositories, type specimens from AGFO, and Monument compliance with museum security policies. Interpretation discusses goals and current implementation for how AGFO interprets its paleontological resources for the public. This includes: the primary themes for interpretation; the exhibits, tools and programs used by AGFO to interpret fossil resources; the target audiences for the interpretive programs; teaching good stewardship of paleontological resources; and a discussion of public accessibility to AGFO, its interpretive materials, and the paleontological resources. Relation of Paleontological Resources to Other Park Programs provides an overview of how each park division may interact with paleontological resources and have their duties cross over with paleontological resource management related actions. It also discusses the potential impacts of paleontological resource management on other types of AGFO resources (archeological, biological, historical, and physical). Paleontological Resource Data Management discusses various paleontological resource records, datasets, and other archives. AGFO’s paleontological archives and library, as well as their current status, are discussed along with an overview of the type of contents stored within them. The NPS Paleontology Program Archives and Library, and how to exchange data and records between them and the AGFO archives, are also described. This section also reviews geospatial data available to AGFO and issues of sensitivity and confidentially related to paleontological resource data and information. Finally, the Summary, Conclusions, and Recommendations summarizes the work done on the AGFO PRMP, discusses ongoing and planned projects which assist in implementing the instructions and goals set out in this PRMP, and makes a variety of recommendations for future paleontological resource management at AGFO

The effect of electrical neurostimulation on collateral perfusion during acute coronary occlusion

<p>Abstract</p> <p>Background</p> <p>Electrical neurostimulation can be used to treat patients with refractory angina, it reduces angina and ischemia. Previous data have suggested that electrical neurostimulation may alleviate myocardial ischaemia through increased collateral perfusion. We investigated the effect of electrical neurostimulation on functional collateral perfusion, assessed by distal coronary pressure measurement during acute coronary occlusion. We sought to study the effect of electrical neurostimulation on collateral perfusion.</p> <p>Methods</p> <p>Sixty patients with stable angina and significant coronary artery disease planned for elective percutaneous coronary intervention were split in two groups. In all patients two balloon inflations of 60 seconds were performed, the first for balloon dilatation of the lesion (first episode), the second for stent delivery (second episode). The Pw/Pa ratio (wedge pressure/aortic pressure) was measured during both ischaemic episodes. Group 1 received 5 minutes of active neurostimulation before plus 1 minute during the first episode, group 2 received 5 minutes of active neurostimulation before plus 1 minute during the second episode.</p> <p>Results</p> <p>In group 1 the Pw/Pa ratio decreased by 10 ± 22% from 0.20 ± 0.09 to 0.19 ± 0.09 (p = 0.004) when electrical neurostimulation was deactivated. In group 2 the Pw/Pa ratio increased by 9 ± 15% from 0.22 ± 0.09 to 0.24 ± 0.10 (p = 0.001) when electrical neurostimulation was activated.</p> <p>Conclusion</p> <p>Electrical neurostimulation induces a significant improvement in the Pw/Pa ratio during acute coronary occlusion.</p

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals &lt;1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Iedereen doet alles.&nbsp; Een blik op de competenties van &nbsp;GGZ-professionals in een context van de&iuml;nstitutionalisering

De paradigmashift van een institutionele geestelijke gezondheidszorg naar meer gemeenschapsgerichte zorg geënt op een herstelgerichte visie hertekent het landschap van de geestelijke gezondheidszorg. Dit impliceert dat de rol, competenties en vaardigheden waarover hulpverleners beschikken eveneens moeten evolueren. Ook de beroepsidentiteiten dreigen te eroderen gezien er meer gekozen wordt voor transdisciplinaire werkvormen. Met dit onderzoek willen we nagaan welke competenties leidinggevenden uit uiteenlopende settings binnen de geestelijke gezondheidszorg noodzakelijk achten voor basiswerkers om in deze nieuwe geestelijke gezondheidsstructuren te werken. Ook wensen we te exploreren wat volgens leidinggevenden en experten de rol van de beroepsidentiteit nog is in deze structuren.    27 participanten bestaande uit 23 leidinggevenden uit drie Belgische GGZ-regio’s en vier experten werden via semigestructureerde interviews bevraagd welke competenties zij als noodzakelijk zien. De ad verbatim getranscribeerde interviews werden via de principes van een inhoudsanalyse geanalyseerd. Zelfsturende organisatievormen sluiten beter aan bij de realiteit van het werkveld. De leidinggevenden benadrukken nieuwe generieke competenties die nodig zijn bij de vermaatschappelijking zoals zelfsturing en netwerking. Beroepsspecificiteit blijft een belangrijke rol spelen, maar is moeilijker benoembaar. Dit uit zich niet in het toekennen van specifieke competenties aan een specifieke beroepsgroep, wel in de blikrichting die deze beroepsgroep heeft, gekleurd door de gehanteerde theoretische kaders

A capital role for the brain’s insula in the diverse fibromyalgia-associated symptoms

Unexplained yet persisting general and widespread non-articular musculoskeletal pain and the associated complaints, known as fibromyalgia (FM), is a common disorder with major social and economic impact. We postulate that in FM disturbance of neurotransmitter balances at the brain’s insula not only leads to aberrant pain processing but could also govern other associated symptoms. Symptoms might arise from central nervous system dysregulation mediated through an imbalance between the excitatory neurotransmitter glutamate and the inhibitory transmitter gamma-amino butyric acid. The insula could also have a leading role in the dysregulation of heart rate and blood pressure, bladder and bowel symptoms, and anxiety and sleep disturbances which are experienced by many FM patients. The presented hypothesis explains how the diverse FM-associated symptoms could be linked, and puts the brain’s insula forward as a possible therapeutic target to be further explored for FM

Exploring methane-oxidizing communities for the co-metabolic degradation of organic micropollutants

Methane-oxidizing cultures from five different inocula were enriched to be used for co-metabolic degradation of micropollutants. In a first screening, 18 different compounds were tested for degradation with the cultures as well as with four pure methane-oxidizing bacterial (MOB) strains. The tested compounds included pharmaceuticals, chemical additives, pesticides, and their degradation products. All enriched cultures were successful in the degradation of at least four different pollutants, but the compounds degraded most often were sulfamethoxazole (SMX) and benzotriazole (BTZ). Addition of acetylene, a specific methane monooxygenase (MMO) inhibitor, revealed that SMX and BTZ were mainly degraded co-metabolically by the present MOB. The pure MOB cultures exhibited less degradation potential, while SMX and BTZ were also degraded by three of the four tested pure strains. For MOB, copper (Cu2+) concentration is often an important factor, as several species have the ability to express a soluble MMO (sMMO) if the Cu2+ concentration is low. In literature, this enzyme is often described to have a broader compound range for co-metabolic degradation of pollutants, in particular when it comes to aromatic structures. However, this study indicated that co-metabolic degradation of the aromatic compounds SMX and BTZ was possible at high Cu2+ concentration, most probably catalyzed by pMMO