Chemical abundance study of two strongly s-process enriched post-AGB stars in the LMC: J051213.81-693537.1 and J051848.86-700246.9

Context: This paper is part of a larger project in which we study the chemical abundances of extra-galactic post-AGB stars with the ultimate goal of improving our knowledge of the poorly understood AGB third dredge-up mixing processes and s-process nucleosynthesis. Aims: In this paper, we study two carefully selected post-AGB stars in the LMC. The combination of favourable atmospheric parameters for detailed abundance studies and their known distances make these objects ideal probes of the internal AGB third dredge-up and s-process nucleosynthesis in that they provide observational constraints for theoretical AGB models. Methods: We use high-resolution optical UVES spectra to determine accurate stellar parameters and perform detailed elemental abundance studies. Additionally, we use available photometric data to construct SEDs for reddening and luminosity determinations. We then estimate initial masses from theoretical post-AGB tracks. Results: Both stars show extreme s-process enrichment associated with relatively low C/O ratios of about 1.3. We could only derive upper limits of the lead (Pb) abundance which indicate no strong Pb overabundances with respect to other s-elements. Comparison with theoretical post-AGB evolutionary tracks in the HR-diagram reveals that both stars have low initial masses between 1.0 and 1.5 Msun. Conclusion: This study adds to the results obtained so far on a very limited number of s-process enriched post-AGB stars in the Magellanic Clouds. We find an increasing discrepancy between observed and predicted Pb abundances towards lower metallicities for all studied Magellanic Cloud post-AGB stars found so far, as well as moderate C/O ratios. We find that all s-process rich post-AGB stars in the LMC and SMC studied so far, cluster in the same region of the HR-diagram and are associated with low-mass stars with a low metallicity on average.Comment: 11 pages, 14 figure

Segment Grammar: A formalism for incremental sentence generation

Incremental sentence generation imposes special constraints on the representation of the grammar and the design of the formulator (the module which is responsible for constructing the syntactic and morphological structure). In the model of natural speech production presented here, a formalism called Segment Grammar is used for the representation of linguistic knowledge. We give a definition of this formalism and present a formulator design which relies on it. Next, we present an object- oriented implementation of Segment Grammar. Finally, we compare Segment Grammar with other formalisms

Post-AGB stars in the Magellanic Clouds and neutron-capture processes in AGB stars

We explore modifications to the current scenario for the slow neutron capture process in asymptotic giant branch (AGB) stars to account for the Pb deficiency observed in post-AGB stars of low metallicity ([Fe/H] ~ -1.2) and low initial mass (~ 1 - 1.5 Msun) in the Large and Small Magellanic Clouds. We calculated the stellar evolution and nucleosynthesis for a 1.3 Msun star with [Fe/H]=-1.3 and tested different amounts and distributions of protons leading to the production of the main neutron source within the 13C-pocket and proton ingestion scenarios. No s-process models can fully reproduce the abundance patterns observed in the post-AGB stars. When the Pb production is lowered the abundances of the elements between Eu and Pb, such as Er, Yb, W, and Hf, are also lowered to below those observed. Neutron-capture processes with neutron densities intermediate between the s and the rapid neutron-capture processes may provide a solution to this problem and be a common occurrence in low-mass, low-metallicity AGB stars.Comment: 6 pages, 4 figures. To be published in Astronomy and Astrophysic

New physical and chemical approaches for the cytosolic delivery of bio- therapeutics and nanoparticles into cells

Delivery of bio-therapeutics and nanomaterials into living cells is an important step not only for cell studies but also for therapy and bio-imaging. Clear examples are the intracellular delivery of various classes of nucleic acids (siRNA, µRNA, mRNA, pDNA), peptides and proteins for therapy purposes. As another example, all types of (inorganic/organic) nanoparticles are under investigation as intracellular labels for imaging purposes. Meanwhile it generally accepted that after uptake by cells, nanomaterials typically end up in endo-lysosomal vesicles in which they remain entrapped while they should escape from such compartments and arrive in the cytosolic fluids of the cells. In recent years our team undertook major efforts to understand the biophysics which play a role in (a lack of) escape of nanomaterials from endo-lysosomal vesicles. Vere recently we also discovered new chemical strategies (so named ‘escape adjuvants’) (1) which seems promising to ‘liberate’ nucleic acids (like siRNA) from endo-lysosomal vesicles into the cytosol. Furthermore we explored physical methods (either light (2,3) or ultrasound (4) driven) which directly deliver bio-therapeutics into the cytosol, thereby bypassing the endo-lysosomal routes. This lecture will explain our recent findings in this area, as reported in a serious of recently published papers (1-4). Both pharmaceutical, biological and engineering aspects of our work will be highlighted in the lecture. References 1) Repurposing cationic amphiphilic drugs as adjuvants to induce lysosomal siRNA escape in nanogel transfected cells F. Joris, L. De Backer, T. Van de Vyver, C. Bastiancich, S.C. De Smedt, K. Raemdonck Journal of Controlled Release 2018, in Press 2) Comparison of gold nanoparticle mediated photoporation: vapour nanobubbles outperform direct heating for delivering macromolecules in live cells R.H. Xiong, K. Raemdonck, K. Peynshaert, I. Lentacker, I. De Cock, J. Demeester, S.C. De Smedt, A.G. Skirtach, K. Braeckmans ACS Nano 2014, 8(6): 6288-6296 3) Cytosolic Delivery of Nanolabels Prevents Their Asymmetric Inhentance and Enables Extended Quantitative in Vivo Cell Imaging R.H. Xiong, F. Joris, S.Y. Liang, R. De Rycke, S. Lippens, J. Demeester, A. Skirtach, K. Raemdonck, U. Himmelreich, S.C. De Smedt, K. Braeckmans Nano Letters 2016, 16(10): 5975-5986 4) Sonoprinting and the importance of microbubble loading for the ultrasound mediated cellular delivery of nanoparticles I. De Cock, G.P.R. Lajoinie, M. Versluis, S.C. De Smedt*, I. Lentacker Biomaterials 2016, 83: 294-30