Spherical clustering of users navigating 360{\deg} content

In Virtual Reality (VR) applications, understanding how users explore the omnidirectional content is important to optimize content creation, to develop user-centric services, or even to detect disorders in medical applications. Clustering users based on their common navigation patterns is a first direction to understand users behaviour. However, classical clustering techniques fail in identifying these common paths, since they are usually focused on minimizing a simple distance metric. In this paper, we argue that minimizing the distance metric does not necessarily guarantee to identify users that experience similar navigation path in the VR domain. Therefore, we propose a graph-based method to identify clusters of users who are attending the same portion of the spherical content over time. The proposed solution takes into account the spherical geometry of the content and aims at clustering users based on the actual overlap of displayed content among users. Our method is tested on real VR user navigation patterns. Results show that our solution leads to clusters in which at least 85% of the content displayed by one user is shared among the other users belonging to the same cluster.Comment: 5 pages, conference (Published in: ICASSP 2019 - 2019 IEEE International Conference on Acoustics, Speech and Signal Processing (ICASSP)

Multidisciplinary Approach to Structural/Energy Diagnosis of Historical Buildings: A Case Study

Abstract A synergic approach for the investigations of the historical building performances - with reference to both the structural behavior and the energy performances for the space heating and cooling - is presented. The historical masonry building "Palazzo Bosco Lucarelli", located in Benevento, has been chosen as case study. The structural and energy analyses are carried out in parallel, especially during the identification of the building characteristics through tests and surveys in-situ. For the structural analysis - beyond examinations on materials - some dynamic tests have been used for better assessing a numerical Finite Element model necessary for the verification of the structure safety. Moreover, being necessary a structural refurbishment, also an energy retrofit could be realized. A rigorous evaluation procedure - aimed to guarantee the necessary reliability of numerical predictions - is performed in order to verify the technical and economical convenience of various energy retrofit solutions

Synchronous primary papillary breast cancer, medullary thyroid carcinoma and neuroendocrine tumor in postmenopausal woman.

Multiple endocrine neoplasia are syndromes involving two or more endocrine tissues, often correlated to RET proto-oncogene mutations. We herein present the first reported case of a 57-years-old woman with three synchronous primary cancers of breast (papillary), thyroid (medullary) and pancreas (neuroendocrine), the latter with liver metastasis. The patient first underwent surgery for papillary breast cancer with axillary lymph nodes metastases. A staging whole body computerized tomography (CT) showed a right lateral cervical lymph node, pancreatic inhomogeneity, peri-pancreatic nodes and a single liver metastasis. The poor response to an antracycline and taxane-based chemotherapy, the good performance status of patient, and associated symptoms, suggested a different origin for pancreatic and hepatic lesions. A careful re-evaluation of clinical history, an octreotide-labeled scan and an immunohistochemical analysis, on both hepatic and pancreatic tissues and on laterocervical lymph node, determined the diagnosis of synchronous papillary breast cancer, pancreatic neuroendocrine tumor (pNET) with liver metastasis and an occult medullary thyroid carcinoma in a patient who had proto-oncogene RET wild type

AAV-mediated transcription factor EB (TFEB) gene delivery ameliorates muscle pathology and function in the murine model of Pompe Disease

Pompe disease (PD) is a metabolic myopathy due to acid alpha-glucosidase deficiency and characterized by extensive glycogen storage and impaired autophagy. We previously showed that modulation of autophagy and lysosomal exocytosis by overexpression of the transcription factor EB (TFEB) gene was effective in improving muscle pathology in PD mice injected intramuscularly with an AAV-TFEB vector. Here we have evaluated the effects of TFEB systemic delivery on muscle pathology and on functional performance, a primary measure of efficacy in a disorder like PD. We treated 1-month-old PD mice with an AAV2.9-MCK-TFEB vector. An animal cohort was analyzed at 3 months for muscle and heart pathology. A second cohort was followed at different timepoints for functional analysis. In muscles from TFEB-treated mice we observed reduced PAS staining and improved ultrastructure, with reduced number and increased translucency of lysosomes, while total glycogen content remained unchanged. We also observed statistically significant improvements in rotarod performance in treated animals compared to AAV2.9-MCK-eGFP-treated mice at 5 and 8 months. Cardiac echography showed significant reduction in left-ventricular diameters. These results show that TFEB overexpression and modulation of autophagy result in improvements of muscle pathology and of functional performance in the PD murine model, with delayed disease progression

energy refurbishment of a university building in cold italian backcountry part 1 audit and calibration of the numerical model

Abstract The study provides a methodological approach for designing energy refurbishment measures of buildings, enabling to understand the uncertainty of using numerical modelling and the real impacts due of adopting some energy efficiency technologies. The case study is a University building of the centre of Italy, and the reference scenario has been supported by various in-situ surveys, investigations and evaluations of the indoor comfort. Collected data, together with a comparison with energy bills, has allowed a proper calibration of a numerical model simulated by means EnergyPlus. All this phase is described in this paper, while a second part will discuss the energy retrofit and the building energy optimization

nzeb target for existing buildings case study of historical educational building in mediterranean climate

Abstract A key element of the Energy Performance of Building Directive 2010/31/EU is the introduction of nearly zero energy building (NZEB) standard for new constructions. However, considering the very low rate of new built volume, the major change for achieve the sustainable grow of the European economy, appears to be the renovation of existing building stock. But, is it possible to reach very low or nearly zero energy standard during refurbishment design? Proposed paper tries to answer this question, evaluating if the refurbishment of historic architectures to very low energy need is possible and economically feasible. With reference to a case study, this paper investigates the cost-optimal energy refurbishment of a Renaissance-style palace, located in the center of Naples, South Italy. The adopted methodology consists of various steps. Firstly, a model of the building has been accurately built and calibrated. Then, it has been used to evaluate possible interventions concerning both the envelope and the energy systems. The best solutions, chosen according to the European methodology of cost-optimality, have been combined in a last simulation. The results show that great energy savings as well as economic and environmental improvements are possible, although heritage buildings present a less flexibility in the proposal of energy efficiency measures

energy refurbishment of a university building in cold italian backcountry part 2 sensitivity studies and optimization

Abstract The first part of this study provided a discussion about the methodological approach for designing energy refurbishment measures of buildings. The case study is a building owned by University of Molise, in Campobasso, a cold Italian city. The reference scenario is a numerical model built after deep investigations, and thus surveys, questionnaires, documents and experimental measurements on the real building. Then, a calibrated energy model was presented. In this second part, starting from the calibrated model, some energy retrofit measures have been implemented. The obtained results allow to discuss two key points for researches in matter of energy refurbishment of buildings: a) the importance of using validated models to simulate the present performance; b) the environmental benefits and the economic implications of a deep energy refurbishment

Amino acids of the Sulfolobus solfataricus mini-chromosome maintenance-like DNA helicase involved in DNA binding/remodeling.

Herein we report the identification of amino acids of the Sulfolobus solfataricus mini-chromosome maintenance (MCM)-like DNA helicase (SsoMCM), which are critical for DNA binding/remodeling. The crystallographic structure of the N-terminal portion (residues 2–286) of the Methanothermobacter thermoautotrophicum MCM protein revealed a dodecameric assembly with two hexameric rings in a head-to-head configuration and a positively charged central channel proposed to encircle DNA molecules. A structure-guided alignment of the M. thermoautotrophicum and S. solfataricus MCM sequences identified positively charged amino acids in SsoMCM that could point to the center of the channel. These residues (Lys-129, Lys-134, His-146, and Lys-194) were changed to alanine. The purified mutant proteins were all found to form homo-hexamers in solution and to retain full ATPase activity. K129A, H146A, and K194A SsoMCMs are unable to bind DNA either in single- or double-stranded form in band shift assays and do not display helicase activity. In contrast, the substitution of lysine 134 to alanine affects only binding to duplex DNA molecules, whereas it has no effect on binding to single-stranded DNA and on the DNA unwinding activity. These results have important implications for the understanding of the molecular mechanism of the MCM DNA helicase action