Suppression of nuclear factor-κB activity in macrophages by chylomicron remnants: modulation by the fatty acid composition of the particles

Current evidence indicates that chylomicron remnants (CMR) induce macrophage foam cell formation, an early event in atherosclerosis. Inflammation also plays a part in atherogenesis and the transcription factor nuclear factor-κB (NF-κB) has been implicated. In this study, the influence of CMR on the activity of NF-κB in macrophages and its modulation by the fatty acid composition of the particles were investigated using macrophages derived from the human monocyte cell line THP-1 and CMR-like particles (CRLPs). Incubation of THP-1 macrophages with CRLPs caused decreased NF-κB activation and downregulated the expression of phospho-p65–NF-κB and phospho-IκBα (pIκBα). Secretion of the inflammatory cytokines tumour necrosis factor α, interleukin-6 and monocyte chemoattractant protein-1, which are under NF-κB transcriptional control, was inhibited and mRNA expression for cyclooxygenase-2, an NF-κB target gene, was reduced. CRLPs enriched in polyunsaturated fatty acids compared with saturated or monounsaturated fatty acids had a markedly greater inhibitory effect on NF-κB binding to DNA and the expression of phospho-p65–NF-κB and pIκB. Lipid loading of macrophages with CRLPs enriched in polyunsaturated fatty acids compared with monounsaturated fatty acids or saturated fatty acids also increased the subsequent rate of cholesterol efflux, an effect which may be linked to the inhibition of NF-κB activity. These findings demonstrate that CMR suppress NF-κB activity in macrophages, and that this effect is modulated by their fatty acid composition. This downregulation of inflammatory processes in macrophages may represent a protective effect of CMR which is enhanced by dietary polyunsaturated fatty acids

Leaf Anatomy and Photochemical Behaviour of Solanum lycopersicum

Plants can be exposed to ionising radiation not only in Space but also on Earth, due to specific technological applications or after nuclear disasters. The response of plants to ionising radiation depends on radiation quality/quantity and/or plant characteristics. In this paper, we analyse some growth traits, leaf anatomy, and ecophysiological features of plants of Solanum lycopersicum L. “Microtom” grown from seeds irradiated with increasing doses of X-rays (0.3, 10, 20, 50, and 100 Gy). Both juvenile and compound leaves from plants developed from irradiated and control seeds were analysed through light and epifluorescence microscopy. Digital image analysis allowed quantifying anatomical parameters to detect the occurrence of signs of structural damage. Fluorescence parameters and total photosynthetic pigment content were analysed to evaluate the functioning of the photosynthetic machinery. Radiation did not affect percentage and rate of seed germination. Plants from irradiated seeds accomplished the crop cycle and showed a more compact habitus. Dose-depended tendencies of variations occurred in phenolic content, while other leaf anatomical parameters did not show distinct trends after irradiation. The sporadic perturbations of leaf structure, observed during the vegetative phase, after high levels of radiation were not so severe as to induce any significant alterations in photosynthetic efficiency

Integration of μ-SOFC Generator and ZEBRA Batteries for Domestic Application and Comparison with other μ-CHP Technologies

Abstract This study investigates the possibility to integrate a Solide Oxide Fuel Cell (SOFC) prime mover and ZEBRA batteries, with the aim to fulfill a domestic user energy demand and to reduce the primary energy consumption, thereby, to enhance the total efficiency in a μ-CHP (Combined Heat and Power) application on a yearly basis. A realistic operational sequence of the SOFC-ZEBRA integration has been calculated using simple logic conditions. Both electric and thermal integration have been considered, in order to exploit the SOFC residual heat for the battery stand-by feeding. The key advantage of this system architecture is that the SOFC is operated without major load variations close to constant load, resulting in longer lifetime and thus reducing total costs of operation. Eventually, a comparison with alternative μ-CHP technologies has been carried out, highlighting the SOFC-ZEBRA potential

Biochemical, Physiological and Anatomical Mechanisms of Adaptation of Callistemon citrinus and Viburnum lucidum to NaCl and CaCl2 Salinization

Callistemon citrinus and Viburnum lucidum are very appreciated and widespread ornamental shrubs for their abundant flowering and/or brilliant foliage. The intrinsic tolerance to drought/salinity supports their use in urban areas and in xeriscaping. Despite adaptive responses of these ornamental species to sodium chloride (NaCl) have been extensively explored, little is known on the effects of other salt solution, yet iso-osmotic, on their growth, mineral composition and metabolism. The present research aimed to assess responses at the biochemical, physiological and anatomical levels to iso-osmotic salt solutions of NaCl and CaCl2 to discriminate the effects of osmotic stress and ion toxicity. The two ornamental species developed different salt-tolerance mechanisms depending on the salinity sources. The growth parameters and biomass production decreased under salinization in both ornamental species, independently of the type of salt, with a detrimental effect of CaCl2 on C. citrinus. The adaptive mechanisms adopted by the two ornamental species to counteract the NaCl salinity were similar, and the decline in growth was mostly related to stomatal limitations of net CO2 assimilation rate, together with the reduction in leaf chlorophyll content (SPAD index). The stronger reduction of C. citrinus growth compared to V. lucidum, was due to an exacerbated reduction in net photosynthetic rate, driven by both stomatal and non stomatal limitations. In similar conditions, V. lucidum exhibited other additional adaptive response, such as modification in leaf functional anatomical traits, mostly related to the reduction in the stomata size allowing plants a better control of stomata opening than in C. citrinus. However, C. citrinus plants displayed an increased ability to retain higher Cl- levels in leaves than in roots under CaCl2 salinity compared to V. lucidum, thus, indicating a further attempt to counteract chloride toxicity through an increased vacuolar compartmentalization and to take advantages of them as chip osmotica

Pump Hydro Storage and Gas Turbines Technologies Combined to Handle Wind Variability: Optimal Hydro Solution for an Italian Case Study☆

Abstract Load and wind energy profiles are totally uncorrelated, therein lies the problem of variable energy sources. Managing load with increasing wind penetration may call for operational ranges that conventional systems cannot readily access. Storage technologies could allow tolerating the unsteadiness of renewable sources with smaller fossil fuel plants capacity. Pumped Hydro Storage (PHS) is a crucial technology for balancing large steam power plants and may become increasingly important for storing renewable energies. Hence capacity ranges of PHS as well as its dynamic response to renewable power variability, will become progressively relevant. An integrated system made of a wind farm, a PHS plant and a set of gas turbines (GTs), as programmable fossil fuel devices, to handle renewable variability and maximize renewable energy exploitation, is studied in this paper. A specific case study is analyzed: a wind farm with a nameplate capacity equal to that installed in Sardinia is considered. To match the power output requested by the region with the integrated systems different configurations of PHS plant will be investigated. The impact of reversible or separate Francis machines with constant or variable speed will be analyzed in order to minimize electric power output overproduction and GTs fuel consumptions. Minimum and maximum capacity range for reversible or separate machines will be considered. The aim of the study is the optimum sizing and design of a PHS unit in a hybrid wind-hydro-gas turbine power plant to match the load request. Results in terms of PHS operation, water height behavior in upper and lower reservoirs, GT units power output, natural gas consumed and electric power output overproduction will be presented for each analyzed case

Environmental Assessment of Renewable Fuel Energy Systems with Cross-Media Effects Approach☆

Abstract In the last years, the number of installed biofuels power plants is increased in northern Italy, due to favorable legislation on renewable energy sources, posing the issue to assess the resulting environmental effects. The European legislation on emissions for renewable fuels power plants provides guidelines to be integrated in the local regulations; moreover, local authorities have to identify the critical power plants in terms of pollution and the key parameters to grant licenses for the future plants. The aim of this paper is to describe a methodology and the calculation routine developed to assess the environmental effects of biomass plants in terms of simple indexes. The used approach is based on the Cross-Media Effects described by a European Commission Reference Document. In particular, several indexes are introduced to cover the most relevant environmental effects, as: air toxicity, global warming, acidification, eutrophication and photochemical ozone creation. For every considered pollutant (such as NOx, CO, etc.) directly emitted by the power plant, specific factors have been identified, in order to calculate the contribution to the different environmental indexes. Finally, a numerical evaluation of different biomass power plants, installed in Emilia Romagna region, is provided, in order to assess their environmental cross-media potential and to compare such kind of power plants with large scale, fossil-fuelled power plants

Control strategy and performance of a small-size thermally integrated Carnot battery based on a Rankine cycle and combined with district heating

To encourage decarbonization and promote a widespread penetration of renewable energy sources in all energy sectors, the development of efficient energy storage systems is essential. Interesting grid-scale electricity storage technologies are the Carnot batteries, whose working principle is based on storing electricity in the form of thermal energy. The charging phase is performed through a heat pump cycle, and the discharging phase is conducted through a heat engine. Since both thermal and electric energy flows are involved, Carnot batteries can be adopted to provide more flexibility in heat and power energy systems. To this aim, efficient scheduling strategies are necessary to manage different energy flows. In this context, this work presents a detailed rule-based control strategy to schedule the synergetic work of a 10-kWe reversible heat pump/organic Rankine cycle Carnot battery integrated to a district heating substation and a photovoltaic power plant, to satisfy a local user's thermal and electric demand. The coupling of a Carnot battery with a district heating substation allows for shaving the thermal demand peaks through the thermal energy stored in the Carnot battery storage, allowing for a downsizing of the district heating substation, with a considerable reduction of the investment costs. Due to the multiplicity of the involved energy flows and the numerous modes of operation, a scheduling logic for the Carnot battery has been developed, to minimize the system operating costs, depending on the boundary conditions. To investigate the influence of the main system design parameters, a detailed and accurate model of the Carnot battery is adopted. Two variants of the reference system, with different heat pump cold source arrangements, are investigated. In the first case, the heat pump absorbs thermal energy from free waste heat. In the second case, the heat pump cold source is the return branch of the district heating substation. The simulation results show that, in the first case, the Carnot battery allows the downsizing of the district heating substation by 47 %, resulting in an annual gain of more than 5000 €. About 70 % of the economic benefit is due to the possibility of reducing the power size of the district heating substation, which can be from 300 to more than 500 kW. The payback period is estimated to be lower than 9 years, while in the second case, the Carnot battery is not able to provide a gain. Eventually, the influence of some parameters, such as the photovoltaic power plant surface, the storage volume, the electricity price profile and the reversible heat pump/organic Rankine cycle specific investment cost, on the techno-economic performance of the system, is investigated through a wide sensitivity analysis. According to the results, the photovoltaic panels surface does not significantly affect the economic gain, while the storage capacity strongly affects the system scheduling and the operating costs. Indeed, it is possible to identify that 13 m3 is the size of the storage volume that minimizes the payback period to 8.22 years, for the considered application. An increase in the electricity price without an increase in the thermal energy price leads to a decrease in economic gain because the benefit brought by the downsizing of district heating is less significant on the economic balance. The specific investment cost of the reversible heat pump/organic Rankine cycle does not influence the operating cost; thus, it does not change the Carnot battery management, nor the economic gain. The specific investment cost affects the payback period, which increases from 8.6 years for a specific cost of 2000 €/kWe to 15.7 years for a specific cost of 5000 €/kWe