Fungal susceptibility of bio-based building materials

Bio-based building materials are made from renewable resources and produced with considerably less energy and associated carbon emissions than many traditional building materials. This makes them essential building elements in the much needed transition towards a more sustainable building industry. Many bio-based building materials are (to some extent) biodegradable, an excellent quality at the end of a material’s service life as it solves waste issues, but a less desirable feature during use. When an organic material is exposed to favourable moisture and temperature conditions as well as to degrading organisms, its functional and aesthetic service life can decrease. The risk of fungal decay depends on the environmental conditions and the material resistance. The purpose of this PhD thesis was to determine how different material characteristics affect the decay risk of bio-based building materials. A test method was developed to assess the importance of material chemistry as compared to material structure and moisture dynamics on durability. State-of-the-art methods (LFNMR, IR, X-ray CT) were applied to better understand the moisture dynamics of wood-based panels and bio-based insulation materials and a method was developed to assess the influence of material structure on decay progress with X-ray CT. As material moisture dynamics and structure have a (major) influence on durability and decay risk, there is a great opportunity to tailor bio-based building materials for diversified end uses and moisture conditions, ensuring an increased service life

Assessing the nutrient value of bio-based materials in relation to early fungal growth

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Bio-based building materials : how to unravel the role of material characteristics on fungal susceptibility?

Bio-based materials are gaining importance in the building industry, as the focus on sustainability and life-cycle-assessment has increased substantially over the last decade. Wood and wood-engineered products as well as insulation materials made from cellulose, wood, flax, hemp, etc. are increasingly used. These materials are made from renewable resources and with considerably lower energy consumption than various other building materials, such as insulation polymers, steel and concrete. As steel can corrode and concrete can rot, so can bio-based building materials degrade over time when exposed to those conditions that favour decay. Since fungi cause not only aesthetical degradation, but can also severely compromise the structural integrity of a building component this is critical for any service life approach. Consequently, a proper understanding of the fungal susceptibility of bio-based materials is needed, both for optimal application of bio-based materials as for the design of new materials. Based on a combination of tests we try to unravel the role of the material’s chemical components, structure and moisture dynamics on its fungal susceptibility, as well as the interaction between those material characteristics. In a first test set-up, the ‘paste test’, the material’s structure is removed and fungal growth is assessed over time in 2D, with only the material’s chemical components playing a role. In the second test set-up, the ‘X-ray CT test’, fungal development is assessed non-destructively in 3D with X-ray CT, giving an indication of moisture production and distribution over time, in relation to the material’s structure. By comparing the results, we have a better idea of how much each material characteristic influences fungal susceptibility. This knowledge can then be used for optimising fungal testing of bio-based materials, ensuring optimal application and providing the building industry with the confidence they need to pave the way to a more sustainable future

Analysis of spatio-temporal fungal growth dynamics under different environmental conditions

Traditionally, fungal growth dynamics were assessed manually, limiting the research to a few environmental conditions and/or fungal species. Fortunately, more automated ways of measurement are gaining momentum due to the availability of cheap imaging and processing equipment and the development of dedicated image analysis algorithms. In this paper, we use image analysis to assess the impact of environmental conditions on the growth dynamics of two economically important fungal species, Coniophora puteana and Rhizoctonia solani. Sixteen environmental conditions combining four temperatures (15, 20, 25 and 30°C) and four relative humidity (RH) conditions (65, 70, 75 and 80% RH) were tested. Fungal growth characteristics were extracted from images of the growing fungi, taken at regular points in time. Advanced time series analysis was applied to quantitatively compare the effect of the environmental conditions on these growth characteristics. The evolution of the mycelial area and the number of tips over time resulted in typical sigmoidal growth curves. Other growth characteristics such as the mean hyphal segment length did not vary significantly over time. Temperature and RH usually had a combined effect on the growth dynamics of the mycelial area and the number of tips. When defining optimal growth conditions for a fungus, it is therefore of primordial importance that the effect of temperature and RH is assessed simultaneously. At the most extreme conditions we tested, the mycelium most probably experienced water stress when developing over the inert Petri dish surface. An RH of 65% (independent of temperature) for C. puteana and a temperature of 30 °C (independent of RH) for both C. puteana and R. solani therefore always resulted in limited fungal growth, while the optimal growing conditions were at 20 °C and 75% RH and at 25°C and 80% RH for R. solani and at 20°C and 75% RH for C. puteana. The method applied in this study offers an updated and broader alternative to classical and narrowly focused studies on fungal growth dynamics, and is well suited to efficiently assess the effect of environmental conditions on fungal growth

Counter-intuitive response to water limitation in a southern European provenance of Frangula alnus Mill. in a common garden experiment

Climate change will intensify drought periods during the growing season in Western Europe. We mimicked this prediction by withholding water in summer from young rooted cuttings of Frangula alnus Mill., a common shrub species, originating from different latitudes in Europe (Italy, Belgium and Sweden) and growing in a common garden environment in Belgium. We followed the responses to the drought up to two years after the treatment. Counter-intuitively, the Italian provenance displayed earlier symptoms and stronger effects of water limitation than the other two provenances. A putative higher transpiration in this provenance could be suggested based on a relative larger shoot growth, larger leaves and a higher stomatal density. After the post-drought re-watering, the droughted plants of the Italian provenance entered leaf senescence later than the control plants, likely as a compensation mechanism for the lost growing time. Bud burst in the first year after the drought treatment and leaf senescence in the next autumn were both advanced in the drought treated group when compared with the control plants. Bud burst in the second year after the drought treatment did not display any differentiation anymore between control and drought treated plants. Growth traits also displayed legacies of the water limitation. For example, the drought treated plants showed a lower number of reshoots upon pruning in the year after the drought treatment. Our results suggest that assisted migration from southern Europe to western Europe as a climate change adaptation strategy might not always follow the expected patterns