Neurotransmitters as food supplements: the effects of GABA on brain and behavior

Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the human cortex. The food supplement version of GABA is widely available online. Although many consumers claim that they experience benefits from the use of these products, it is unclear whether these supplements confer benefits beyond a placebo effect. Currently, the mechanism of action behind these products is unknown. It has long been thought that GABA is unable to cross the blood–brain barrier (BBB), but the studies that have assessed this issue are often contradictory and range widely in their employed methods. Accordingly, future research needs to establish the effects of oral GABA administration on GABA levels in the human brain, for example using magnetic resonance spectroscopy. There is some evidence in favor of a calming effect of GABA food supplements, but most of this evidence was reported by researchers with a potential conflict of interest. We suggest that any veridical effects of GABA food supplements on brain and cognition might be exerted through BBB passage or, more indirectly, via an effect on the enteric nervous system. We conclude that the mechanism of action of GABA food supplements is far from clear, and that further work is needed to establish the behavioral effects of GABA

The galaxy environment in GAMA G3C groups using the Kilo Degree Survey Data Release 3

We aim to investigate the galaxy environment in GAMA Galaxy Groups Catalogue (G3C) using a volume-limited galaxy sample from the Kilo Degree Survey Data Release 3. The k-Nearest Neighbour technique is adapted to take into account the probability density functions (PDFs) of photometric redshifts in our calculations. This algorithm was tested on simulated KiDS tiles, showing its capability of recovering the relation between galaxy colour, luminosity and local environment. The characterization of the galaxy environment in G3C groups shows systematically steeper density contrasts for more massive groups. The red galaxy fraction gradients in these groups is evident for most of group mass bins. The density contrast of red galaxies is systematically higher at group centers when compared to blue galaxy ones. In addition, distinct group center definitions are used to show that our results are insensitive to center definitions. These results confirm the galaxy evolution scenario which environmental mechanisms are responsible for a slow quenching process as galaxies fall into groups and clusters, resulting in a smooth observed colour gradients in galaxy systems.Comment: 14 pages, Accepted to MNRA

The counterrotating core and the black hole mass of IC1459

The E3 giant elliptical galaxy IC1459 is the prototypical galaxy with a fast counterrotating stellar core. We obtained one HST/STIS long-slit spectrum along the major axis of this galaxy and CTIO spectra along five position angles. We present self-consistent three-integral axisymmetric models of the stellar kinematics, obtained with Schwarzschild's numerical orbit superposition method. We study the dynamics of the kinematically decoupled core (KDC) in IC1459 and we find it consists of stars that are well-separated from the rest of the galaxy in phase space. The stars in the KDC counterrotate in a disk on orbits that are close to circular. We estimate that the KDC mass is ~0.5% of the total galaxy mass or ~3*10^9 Msun. We estimate the central black hole mass M_BH of IC1459 independently from both its stellar and its gaseous kinematics. Some complications probably explain why we find rather discrepant BH masses with the different methods. The stellar kinematics suggest that M_BH = (2.6 +/- 1.1)*10^9 Msun (3 sigma error). The gas kinematics suggests that M_BH ~ 3.5*10^8 Msun if the gas is assumed to rotate at the circular velocity in a thin disk. If the observed velocity dispersion of the gas is assumed to be gravitational, then M_BH could be as high as ~1.0*10^9 Msun. These different estimates bracket the value M_BH = (1.1 +/- 0.3)*10^9 Msun predicted by the M_BH-sigma relation. It will be an important goal for future studies to assess the reliability of black hole mass determinations with either technique. This is essential if one wants to interpret the correlation between the BH mass and other global galaxy parameters (e.g. velocity dispersion) and in particular the scatter in these correlations (believed to be only ~0.3 dex). [Abridged]Comment: 51 pages, LaTeX with 19 PostScript figures. Revised version, with three new figures and data tables. To appear in The Astrophysical Journal, 578, 2002 October 2

N=2 supergravity in five dimensions revisited

We construct matter-coupled N=2 supergravity in five dimensions, using the superconformal approach. For the matter sector we take an arbitrary number of vector-, tensor- and hyper-multiplets. By allowing off-diagonal vector-tensor couplings we find more general results than currently known in the literature. Our results provide the appropriate starting point for a systematic search for BPS solutions, and for applications of M-theory compactifications on Calabi-Yau manifolds with fluxes.Comment: 35 pages; v.2: A sign changed in a bilinear fermion term in (5.7

Intraobserver and interobserver variability and spatial differences in histologic examination of carotid endarterectomy specimens

IntroductionStudies using histologic examination and protein analysis of atherosclerotic plaques are increasingly being performed, but reproducibility of plaque histology and variation of plaque composition among different parts of the plaque, which are key to reliability of these studies, are relatively unexplored. Therefore, this study investigated the intraobserver and interobserver variability of plaque histology and spatial variability in plaque composition.MethodsAtherosclerotic plaques (n = 100) obtained during carotid endarterectomy were divided into 0.5-cm segments. Paraffin sections were stained and semiquantitatively analyzed (four categories: no, minor, moderate, and heavy) for fat, macrophages, smooth muscle cells, collagen, calcification, thrombus, and overall phenotype. First, to determine the intraobserver and interobserver reproducibility, two independent observers independently analyzed the plaques. Second, to investigate spatial variability in plaque composition, histologic appearances of the culprit lesions (0-segment) were compared with the histologic appearances of adjacent (+5 mm) and more distant (+10 mm) plaque segments of 30 specimens.ResultsThe κ values for intraobserver variability of fat, macrophages, smooth muscle cells, collagen, calcifications, thrombus, and overall phenotype were 0.83, 0.85, 0.71, 0.63, 0.81, 0.80, and 0.86, respectively, and κ values for interobserver variability were 0.68, 0.74, 0.54, 0.59, 0.82, 0.75, and 0.71, respectively. Comparison of the histologic scorings of adjacent segments revealed a mean κ of 0.40 (range, 0.33 to 0.60). When the culprit segment was compared with the more distant segment, the mean κ was 0.24; however, in 91% of cases, the difference between the culprit segment and the distal segment was one category or less.ConclusionSemiquantitative analysis of carotid atherosclerotic plaque histology was well reproducible, both intraobserver and interobserver. Although variation between different plaque segments in histologic appearance was observed, differences were small in almost all cases. Variability in histologic examination needs to be taken into account in studies comparing plaque imaging with histopathology and plaque research studies

DenseLens -- Using DenseNet ensembles and information criteria for finding and rank-ordering strong gravitational lenses,

Convolutional neural networks (CNNs) are the state-of-the-art technique for identifying strong gravitational lenses. Although they are highly successful in recovering genuine lens systems with a high true-positive rate, the unbalanced nature of the data set (lens systems are rare), still leads to a high false positive rate. For these techniques to be successful in upcoming surveys (e.g. with Euclid) most emphasis should be set on reducing false positives, rather than on reducing false negatives. In this paper, we introduce densely connected neural networks (DenseNets) as the CNN architecture in a new pipeline-ensemble model containing an ensemble of classification CNNs and regression CNNs to classify and rank-order lenses, respectively. We show that DenseNets achieve comparable true positive rates but considerably lower false positive rates (when compared to residual networks; ResNets). Thus, we recommend DenseNets for future missions involving large data sets, such as Euclid, where low false positive rates play a key role in the automated follow-up and analysis of large numbers of strong gravitational lens candidates when human vetting is no longer feasibl

A comparison of the Thunderbeat and standard electrocautery devices in head and neck surgery:a prospective randomized controlled trial

PURPOSE: New energy-based sutureless vessel ligation devices, such as the Thunderbeat (Olympus Medical Systems Corp., Tokyo, Japan), could reduce operative time and limit blood loss in head and neck surgery; however, efficacy and safety in major head and neck surgery have not been investigated in a prospective, randomized study. METHODS: This prospective, double-arm, randomized controlled trial consisted of two parts: total laryngectomy (TL) and neck dissection (ND). Thirty patients planned for TL were randomized in two groups. For the ND part, forty-two operative sides were likewise randomized. In both parts, Thunderbeat was used in addition to the standard instrumentation in the intervention groups, while only standard instrumentation was used in the control groups. Primary outcome values were blood loss, operative time and complication rate. RESULTS: For the TL part there was no difference in mean blood loss (p = 0.062), operative time (p = 0.512) and complications (p = 0.662) between both hemostatic techniques. For the neck dissection part, there was a reduction in blood loss (mean 210 mL versus 431 mL, p = 0.046) and in operative time (median 101 (IQR 85-130) minutes versus 150 (IQR 130-199) minutes, p = 0.014) when Thunderbeat was used. There was no difference in complication rate between both hemostatic systems (p = 0.261). CONCLUSION: The Thunderbeat hemostatic device significantly reduces operative blood loss and operative time for neck dissections, without increase in complications. In TL, blood loss using Thunderbeat was comparable with the standard technique, but the operative time tended to be shorter. TRIAL REGISTRATION: UMCG Research Register, Reg. no. 201700041, date of registration: 18/1/2017

Strategic design of long-term climate policy instrumentations, with exemplary EU focus

Industrial Ecolog

Virtual 3D planning of tracheostomy placement and clinical applicability of 3D cannula design:A three-step study

AIM: We aimed to investigate the potential of 3D virtual planning of tracheostomy tube placement and 3D cannula design to prevent tracheostomy complications due to inadequate cannula position. MATERIALS AND METHODS: 3D models of commercially available cannula were positioned in 3D models of the airway. In study (1), a cohort that underwent tracheostomy between 2013 and 2015 was selected (n = 26). The cannula was virtually placed in the airway in the pre-operative CT scan and its position was compared to the cannula position on post-operative CT scans. In study (2), a cohort with neuromuscular disease (n = 14) was analyzed. Virtual cannula placing was performed in CT scans and tested if problems could be anticipated. Finally (3), for a patient with Duchenne muscular dystrophy and complications of conventional tracheostomy cannula, a patient-specific cannula was 3D designed, fabricated, and placed. RESULTS: (1) The 3D planned and post-operative tracheostomy position differed significantly. (2) Three groups of patients were identified: (A) normal anatomy; (B) abnormal anatomy, commercially available cannula fits; and (C) abnormal anatomy, custom-made cannula, may be necessary. (3) The position of the custom-designed cannula was optimal and the trachea healed. CONCLUSIONS: Virtual planning of the tracheostomy did not correlate with actual cannula position. Identifying patients with abnormal airway anatomy in whom commercially available cannula cannot be optimally positioned is advantageous. Patient-specific cannula design based on 3D virtualization of the airway was beneficial in a patient with abnormal airway anatomy