Neurotransmitter Detection Using Corona Phase Molecular Recognition on Fluorescent Single-Walled Carbon Nanotube Sensors

ABSTRACT: Temporal and spatial changes in neurotransmitter concentrations are central to information processing in neural networks. Therefore, biosensors for neurotransmitters are essential tools for neuroscience. In this work, we applied a new technique, corona phase molecular recognition (CoPhMoRe), to identify adsorbed polymer phases on fluorescent single-walled carbon nanotubes (SWCNTs) that allow for the selective detection of specific neurotransmitters, including dopamine. We functionalized and suspended SWCNTs with a library of different polymers (n = 30) containing phospholipids, nucleic acids, and amphiphilic polymers to study how neurotransmitters modulate the resulting band gap, near-infrared (nIR) fluorescence of the SWCNT. We identified several corona phases that enable the selective detection of neurotransmitters. Catecholamines such as dopamine increased the fluorescence of specific single-stranded DNA- and RNA-wrapped SWCNTs by 58−80 % upon addition of 100 μM dopamine depending on the SWCNT chirality (n,m). In solution, the limit of detection was 11 nM [Kd = 433 nM for (GT)15 DNA-wrapped SWCNTs]. Mechanistic studies revealed that this turn-on response is due to an increase in fluorescence quantum yield and not covalent modification of the SWCNT or scavenging o

ECLAIRE third periodic report

The ÉCLAIRE project (Effects of Climate Change on Air Pollution Impacts and Response Strategies for European Ecosystems) is a four year (2011-2015) project funded by the EU's Seventh Framework Programme for Research and Technological Development (FP7)

Becoming Animal film

Audiovisual file for the film Becoming Animal.An inspired collaboration between filmmakers Emma Davie (I Am Breathing) and Peter Mettler (The End of Time) and radical writer and philosopher David Abram (The Spell of the Sensuous), Becoming Animal is an urgent and immersive audiovisual quest, forging a path into the places where humans and other animals meet, where we pry open our senses to witness the so-called natural world—which in turn witnesses us, prompting us to reflect on the very essence of what it means to inhabit our animal bodies. Shot in and around Grand Teton National Park, with its dizzying diversity of wildlife, trails of curious humans in RV’s and billion-year-old geology, the film is a geyser of provocative ideas and heightened sensations related to the sublime circuitry that connects us to our ever-shifting surroundings. A snail’s body becomes an immense landscape as the soundscape immerses us in shivering leaves, rushing rivers and the weird spacey pitch of elk bugling at night. Becoming Animal embraces the sensory tools of cinema to trace how the written word and technology has affected how we see. Driven by wonder, curiosity and a desire for balance between ecological and technological imperatives, Becoming Animal is an invitation to explore our relationship with this “more than human world” and recognise it for what it is: an exquisitely intricate system in which everything is alive and expressive, humans, animals and landscapes are inextricably interdependent, and there is no such thing as empty space. Countries: Switzerland / UK Year: 2018 Duration: 78min Format: DCP / BluRay Language: English Production Companies: Maximage / SDI Productions Ltd ## Access ## This dataset is held in the Edinburgh DataVault, directly accessible only to authorised University of Edinburgh users. External users may request access to a copy of the data by contacting the Principal Investigator, Contact Person or Data Manager named on this page or email CAHSS REF: [email protected]. University of Edinburgh users who wish to have direct access should consult the information about retrieving data from the DataVault at: http://www.ed.ac.uk/is/research-support/datavault .Davie, Emma; Mettler, Peter "Becoming Animal film" (2019) Edinburgh DataVault https://doi.org/10.7488/6fee4875-894f-4613-a861-59d5e46d859