44 research outputs found

    Advances in methods for detection of anaerobic ammonium oxidizing (anammox) bacteria

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    Anaerobic ammonium oxidation (anammox), the biochemical process oxidizing ammonium into dinitrogen gas using nitrite as an electron acceptor, has only been recognized for its significant role in the global nitrogen cycle not long ago, and its ubiquitous distribution in a wide range of environments has changed our knowledge about the contributors to the global nitrogen cycle. Currently, several groups of methods are used in detection of anammox bacteria based on their physiological and biochemical characteristics, cellular chemical composition, and both 16S rRNA gene and selective functional genes as biomarkers, including hydrazine oxidoreductase and nitrite reductase encoding genes hzo and nirS, respectively. Results from these methods coupling with advances in quantitative PCR, reverse transcription of mRNA genes and stable isotope labeling have improved our understanding on the distribution, diversity, and activity of anammox bacteria in different environments both natural and engineered ones. In this review, we summarize these methods used in detection of anammox bacteria from various environments, highlight the strengths and weakness of these methods, and also discuss the new development potentials on the existing and new techniques in the future

    Denitrification in drained and rewetted minerotrophic peat soils in Northern Germany (Pohnsdorfer Stauung)

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    This study was conducted to assess the nitrogen removal potential of a minerotrophic peatland in Northern Germany, where hydrological conditions were partly restored in the beginning of the 1990s. Actual denitrification and the effect of nitrate (NO3-) and glucose additions on denitrification rates were determined in two flooded and one drained histosols in spring and summer 1998. In the flooded soils, denitrification was insignificant, but the drained field emitted significant rates. Additions of NO3- stimulated denitrification at all sites in spring and summer, whereas glucose additions had no effect. Low NO3- concentration in floodwater was obviously limiting denitrification in the flooded soils. In the drained soil, a coupled nitrification/denitrification might explain the low, but significant denitrification rates. No spontaneous production of nitrous oxide occurred in the flooded soils, whereas at the drained site an increase in spontaneous nitrous oxide concentration was measured during incubation in the summer samples. The suggested introduction of NO3- rich water from a stream flowing through the area would apparently induce denitrification in the flooded fields

    Impact of drying and re-wetting on N, P and K dynamics in a wetland soil

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    As increased nutrient availability due to drainage is considered a major cause of eutrophication in wetlands rewetting of drained wetlands is recommended as a restoration measure. The effect of soil drying and rewetting on the contribution of various nutrient release or transformation processes to changed nutrient availability for plants is however weakly understood. We measured effects of soil drying and re-wetting on N mineralization, and denitrification, as well as on release of dissolved organic nitrogen (DON), phosphorus, and potassium in incubated soil cores from a wet meadow in southern Sweden. Additionally, the impact of re-wetting with sulphate-enriched water was studied. Soil drying stimulated N mineralization (3 times higher) and reduced denitrification (5 times lower) compared to continuously wet soil. In the wet cores, denitrification increased to 20 mg N m(-2) d(-1), which was much higher than denitrification measured in the field. In the field, increased inorganic-N availability for plants due to drainage seemed primarily to be caused by increased N mineralization, and less by decreased denitrification. Soil drying also stimulated the release of DON and K, but P release was not affected. Re-wetting of dried soil cores strongly stimulated denitrification (up to 160 mg N m(-2) d(-1)), but N mineralization was not significantly decreased, neither were DON or K release. In contrast, the extractable P pool increased upon soil wetting. Re-wetting with sulphate-enriched water had no effect on any of the nutrient release or transformation rates. We conclude that caution is required in re-wetting of drained wetlands, because it may unintendently cause internal eutrophication through an increased P availability for plants

    Strategies for superior performance under adverse conditions: a focus on small and medium-sized high-growth firms

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    This article explores the strategies pursued by small and medium-sized firms to actively sustain growth within declining markets. A critical analysis of relevant growth theories informs the development of a semi-structured interview schedule; findings drawn from 20 case studies indicate that firms adopt a multiple-strategy approach in which they simultaneously pursue an innovative differentiation and product or service-customisation strategy. Following this strategy, it was found that firms make an intentional search for high-margin products, avoid aggressive price competition and maintain tight control of costs. The article demonstrates that an adverse environment does not necessarily inhibit firm growth and that individual firm-specific strategies can be invoked to overcome volatile market conditions
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