Biofilm monitoring coupon system and method of use

An apparatus and method is disclosed for biofilm monitoring of a water distribution system which includes the mounting of at least one fitting in a wall port of a manifold in the water distribution system with a passage through the fitting in communication. The insertion of a biofilm sampling member is through the fitting with planar sampling surfaces of different surface treatment provided on linearly arrayed sample coupons of the sampling member disposed in the flow stream in edge-on parallel relation to the direction of the flow stream of the manifold under fluid-tight sealed conditions. The sampling member is adapted to be aseptically removed from or inserted in the fitting and manifold under a positive pressure condition and the fitting passage sealed immediately thereafter by appropriate closure means so as to preclude contamination of the water distribution system through the fitting. The apparatus includes means for clamping the sampling member and for establishing electrical continuity between the sampling surfaces and the system for minimizing electropotential effects. The apparatus may also include a plurality of fittings and sampling members mounted on the manifold to permit extraction of the sampling members in a timed sequence throughout the monitoring period

A Path to Alignment: Connecting K-12 and Higher Education via the Common Core and the Degree Qualifications Profile

The Common Core State Standards (CCSS), which aim to assure competency in English/language arts and mathematics through the K-12 curriculum, define necessary but not sufficient preparedness for success in college. The Degree Qualifications Profile (DQP), which describes what a college degree should signify, regardless of major, offers useful but not sufficient guidance to high school students preparing for college study. A coordinated strategy to prepare students to succeed in college would align these two undertakings and thus bridge an unfortunate and harmful cultural chasm between the K-12 world and that of higher education. Chasms call for bridges, and the bridge proposed by this white paper could create a vital thoroughfare. The white paper begins with a description of the CCSS and an assessment of their significance. A following analysis then explains why the CCSS, while necessary, are not sufficient as a platform for college success. A corresponding explanation of the DQP clarifies the prompts that led to its development, describes its structure, and offers some guidance for interpreting the outcomes that it defines. Again, a following analysis considers the potential of the DQP and the limitations that must be addressed if that potential is to be more fully realized. The heart of the white paper lies in sections 5 and 6, which provide a crosswalk between the CCSS and the DQP. These sections show how alignments and differences between the two may point to a comprehensive preparedness strategy. They also offer a proposal for a multifaceted strategy to realize the potential synergy of the CCSS and the DQP for the benefit of high school and college educators and their students -- and the nation

‘Who? Me. A Memoir’ Donald Leslie Shaw (1930–2017)

No abstract available

Controlled cortical impact traumatic brain injury in 3xTg-AD mice causes acute intra-axonal amyloid-β accumulation and independently accelerates the development of tau abnormalities

Alzheimer\u27s disease (AD) is a neurodegenerative disorder characterized pathologically by progressive neuronal loss, extracellular plaques containing the amyloid-β (Aβ) peptides, and neurofibrillary tangles composed of hyperphosphorylated tau proteins. Aβ is thought to act upstream of tau, affecting its phosphorylation and therefore aggregation state. One of the major risk factors for AD is traumatic brain injury (TBI). Acute intra-axonal Aβ and diffuse extracellular plaques occur in ∼30% of human subjects after severe TBI. Intra-axonal accumulations of tau but not tangle-like pathologies have also been found in these patients. Whether and how these acute accumulations contribute to subsequent AD development is not known, and the interaction between Aβ and tau in the setting of TBI has not been investigated. Here, we report that controlled cortical impact TBI in 3xTg-AD mice resulted in intra-axonal Aβ accumulations and increased phospho-tau immunoreactivity at 24 h and up to 7 d after TBI. Given these findings, we investigated the relationship between Aβ and tau pathologies after trauma in this model by systemic treatment of Compound E to inhibit γ-secretase activity, a proteolytic process required for Aβ production. Compound E treatment successfully blocked posttraumatic Aβ accumulation in these injured mice at both time points. However, tau pathology was not affected. Our data support a causal role for TBI in acceleration of AD-related pathologies and suggest that TBI may independently affect Aβ and tau abnormalities. Future studies will be required to assess the behavioral and long-term neurodegenerative consequences of these pathologies

XMM-Newton and Chandra Cross Calibration Using HIFLUGCS Galaxy Clusters: Systematic Temperature Differences and Cosmological Impact

Cosmological constraints from clusters rely on accurate gravitational mass estimates, which strongly depend on cluster gas temperature measurements. Therefore, systematic calibration differences may result in biased, instrument-dependent cosmological constraints. This is of special interest in the light of the tension between the Planck results of the primary temperature anisotropies of the CMB and Sunyaev-Zel'dovich plus X-ray cluster counts analyses. We quantify in detail the systematics and uncertainties of the cross-calibration of the effective area between five X-ray instruments, EPIC-MOS1/MOS2/PN onboard XMM-Newton and ACIS-I/S onboard Chandra, and the influence on temperature measurements. Furthermore, we assess the impact of the cross calibration uncertainties on cosmology. Using the HIFLUGCS sample, consisting of the 64 X-ray brightest galaxy clusters, we constrain the ICM temperatures through spectral fitting in the same, mostly isothermal, regions and compare them. Our work is an extension to a previous one using X-ray clusters by the IACHEC. Performing spectral fitting in the full energy band we find that best-fit temperatures determined with XMM-Newton/EPIC are significantly lower than Chandra/ACIS temperatures. We demonstrate that effects like multitemperature structure and different relative sensitivities of the instruments at certain energy bands cannot explain the observed differences. We conclude that using XMM-Newton/EPIC, instead of Chandra/ACIS to derive full energy band temperature profiles for cluster mass determination results in an 8% shift towards lower OmegaM values and <1% shift towards higher sigma8 values in a cosmological analysis of a complete sample of galaxy clusters. Such a shift is insufficient to significantly alleviate the tension between Planck CMB anisotropies and SZ plus XMM-Newton cosmological constraints.Comment: Accepted by A&A; Python-Script for modification of XMM-Newton/EPIC and Chandra/ACIS effective areas according to the stacked residual ratios: https://wikis.mit.edu/confluence/display/iachec/Data

Aquatic Invertebrate Community Structure, Biological Condition, Habitat, and Water Quality at Ozark National Scenic Riverways, Missouri, 2005-2014

Ozark National Scenic Riverways (OZAR) was established to protect the corridor of the Current River and its major tributary, the Jacks Fork. The Current River is one of the few remaining free-flowing rivers in the U.S., with much of its base flow coming from several large springs. To assess the biological condition of these rivers, aquatic invertebrate community structure was monitored from 2005 to 2014. Benthic invertebrate samples and associated habitat and water quality data were collected from each of nine sampling sites using a Slack-Surber sampler. The Stream Condition Index (SCI), a multimetric index that incorporates taxa richness, EPT (Ephemeroptera, Plecoptera, Trichoptera) richness, Shannon’s diversity index, and Hilsenhoff Biotic Index (HBI), was calculated. The benthic invertebrate fauna was diverse with 155 distinct taxa identified from all sites. Mean taxa richness was high, ranging from 22 to 30 among sites. The invertebrate taxa of the Current River and Jacks Fork are largely intolerant across all taxa represented (mean tolerance value= ~4.25). Mean HBI did not exceed 3.9 in the Current River or 4.4 for the Jacks Fork. Mean SCI scores across sampling sites generally were well above 16, indicating they are not impaired. Habitat and water quality data were summarized, but they were poorly correlated with individual invertebrate metrics. Sørenson’s similarity index was used to assess community similarity among sites, and similarity scores were then analyzed using ascendant hierarchical cluster analysis. Similarity among sites was 72% or greater. Cluster analysis showed that Current River and Jacks Fork sites clustered separately and in a downstream progression. The uppermost collection site on the Current River was most unlike the other sites, which probably relates to the distinct physical features of that site compared to the others. Nonmetric Multidimensional Scaling (NMDS) was used to evaluate the relationship of invertebrate metrics to habitat and water quality. The NMDS model was found to be a good fit (stress=0.04) and specific conductance, temperature, discharge, filamentous algae and aquatic vegetation were among the most important habitat variables in defining the relationship among sampling sites. The three lower Current River and Jacks Fork sites each were closely grouped in ordination space, but the three upper Current River sites were farther apart from each other. The influence of several large volume springs near those sites is suspected of producing such disparity through press type disturbances. Although the invertebrate communities and water quality in the Current River and Jacks Fork are largely sound and have high biological condition, ongoing and projected threats to these resources remain, and those threats largely originate outside park jurisdictional boundaries. Inherent variability of invertebrate community diversity across sites and years highlights the importance of using multi-metric assessments and multiyear monitoring to support management decisions

Evolutionary Divergence in Developmental Strategies and Neuromodulatory Control Systems of Two Amphibian Locomotor Networks

Attempts to understand the neural mechanisms which produce behaviour must consider both prevailing sensory cues and the central cellular and synaptic changes they direct. At each level, neuromodulation can additionally shape the final output. We have investigated neuromodulation in the developing spinal motor networks in hatchling tadpoles of two closely related amphibians, Xenopus laevis and Rana temporaria to examine the subtle differences in their behaviours that could be attributed to their evolutionary divergence. At the point of hatching, both species can swim in response to a mechanosensory stimulus, however Rana embryos often display a more forceful, non-locomotory coiling behaviour. Whilst the synaptic drive that underlies these behaviours appears similar, subtle inter-specific differences in neuronal properties shape motor outputs in different ways. For example, Rana neurons express N-methyl-D-aspartate (NMDA)/serotonin (5-HT)-dependent oscillations, not present in hatchling Xenopus and many also exhibit a prominent slow spike after-hyperpolarisation. Such properties may endow the spinal circuitry of Rana with the ability to produce a more flexible range of outputs. Finally, we compare the roles of the neuromodulators 5-HT, noradrenaline (NA) and nitric oxide (NO) in shaping motor outputs. 5-HT increases burst durations during swimming in both Xenopus and Rana, but 5-HT dramatically slows the cycle period in Rana with little effect in Xenopus. Three distinct, but presumably homologous NO-containing brainstem clusters of neurons have been described, yet the effects of NO differ between species. In Xenopus, NO slows and shortens swimming in a manner similar to NA, yet in Rana NO and NA elicit the non-rhythmic coiling pattern