Population Decline of White Locoweed

Many Astragalus and Oxytropis species are endemic (growing on specific soils and geographical areas), but white locoweed (Oxytropis sericea) is the most widespread locoweed in the western United States, growing on short-grass prairies and eastern foothills of the Rocky Mountains from Montana to New Mexico. Its preferred habitat is rocky soils, where its long taproot can access deep percolated water allowing it to survive drought, temperature, and wind stress.The Rangelands archives are made available by the Society for Range Management and the University of Arizona Libraries. Contact [email protected] for further information.Migrated from OJS platform March 202

Confocal laser scanning microscopy of environmental samples

Enormous technical advances in imaging and data acquisition techniques, combined with a continuing increased scope for fluorescence labeling of specific constituents of living organisms, have brought about a revolution in approaches to biological problems. Whereas spatial organisation at an ultrastructural level would have seemed definitely the province of electron microscopy just a few years ago, now the new methods of light microscopy can offer not only a complementary approach, but can achieve more, especially in terms of noninvasive and real-time measurements. Confocal laser scanning microscopy (CLSM) is the most revolutionary development in optical microscopy since the early sevent eenth century. From the microbial ecologist’s point of view, the availability of this powerful new technique, which has been driven primarily by the needs of the biomedical sciences to define in situ in cells the interactions of ions, molecules and macromolecules, with membranes with a minimum of perturbation, could not be more timely. The past century has seen great advances in microbial physiology and biochemistry, mostly with organisms grown in suspension on rich media and at high growth rates. More recently there is a growing awareness that surface growth, nutrient deprivation, or pulsatile nutrient sufficiency are more realistic modes of laboratory cultivation with respect to the natural environment. But the study of heterogeneity in space and time brings a host of methodological problems not encountered in the shake flask or continuously stirred tank reactor

Generative Models for City-Specific Vehicle Trajectories

Modeling vehicle trajectories in cities is an important task that can help transportation planners make smart decisions around traffic policies and transportation infrastructure. Often times there is not enough data available for the planners to use, or the data available contains sensitive personal information which, if used, would violate data privacy policies. Without data, uninformed decisions can lead to traffic congestion and inadequate public transportation infrastructure. With the annual costs of traffic congestion exceeding one trillion U.S. dollars worldwide, this is obviously a problem worth addressing. We propose a method of generating new samples of vehicle trajectory data for a given city, leveraging existing GAN models with novel data representations

Confocal laser scanning microscopy of trichomonads: Hydrogenosomes store calcium and show a membrane potential

Confocal laser scanning microscopy of Tritrichomonas foetus and Trichomonas vaginalis stained with Fluo-3AM a fluorescent calcium-selective probe show distinct intracellular calcium locations. The pattern of localization is comparable with the position of hydrogenosomes previously observed in these trichomonads by electron microscopy. The Ca2+-specific chelator, EGTA, sequestered Ca2+ from these Ca2+ stores when applied to living organisms. Calcium ions were also released from isolated hydrogenosomes when these organelles were diluted in vitro, but no substrate driven uptake of Ca2+ could be detected using a calcium electrode. The subcellular binding of an oxonol dye DiBAC4(3), a fluorescent membrane potential probe observed by confocal laser scanning microscopy, and confirmed by flow cytometric measurements of fluorescence emission of stained hydrogenosomes in vitro, strongly suggests the presence of a transmembrane electrochemical gradient across the membrane of this organelle