GnRH removal in the 7-11 CO-Synch for timed insemination of beef heifers

The use of artificial insemination can improve genetics, shorten the calving season, and increase weaning weights by having more calves born earlier in the breeding season. Reasons that this technology has not been used by many producers may include poor conception rates, time, and cost. Research has been directed at shortening the synchronization time and controlling time of ovulation to eliminate estrous detection using a timed artificial insemination. Use of a progestin, like the Eazi-Breed CIDR or melengestrol acetate (MGA), is desirable because they are effective in synchronizing estrus and they can also induce cyclicity in anestrus or prepubertal females. In addition, gonadotropin releasing hormone (GnRH) is commonly included in a synchronization plan because it induces ovulation and synchronizes follicular development. The most common synchronization protocol for beef heifers consists of feeding MGA for 14 days, a prostaglandin injection 17 to 19 days later followed by five days of heat detection for a total duration of 36 to 38 days. Recent research suggests that acceptable conception rates can be achieved when shortening the time of MGA to seven days. One example is the 7-11 CO-Synch which is comprised of seven days of MGA feeding followed by the CO-Synch protocol starting on day 11 (see Figure 1). We have obtained greater then 60% conception rates using the 7-11 CO-Synch in previous years. The feeding of MGA not only synchronizes the estrous cycle but provides exposure to a progestin that may induce prepubertal heifers to begin cycling. The GnRH injection at day 11 should synchronize the follicular wave which will tighten the timing of ovulation and improve timed insemination conception rates. The disadvantage to this synchronization protocol is that it requires the heifers to go through the chute four times. It is also believed that heifers are less responsive to GnRH than cows. Thus we tested this protocol with or without the day 11 GnRH injection on conception rates of beef heifers

Effect of heifer source on reproductive performance, culling, marketing and profitability for a commercial heifer development program

A commercial heifer development operation purchased 483 weanling Angus × Hereford heifers from 11 sources. Heifers were fed a common silage-based diet through an initial developmental period and retained or culled based on average daily gain, pelvic area, and disposition . The percentage of heifers culled from each source ranged from 18.1% to 94.7% and were either sold directly through a local sale barn or sent to a feedlot with retained ownership . Estrus was synchronized, and heifers were artificially inseminated (AI) for 30 days followed by 15 days of natural mating. First service conception rates for each source ranged from 0% to 92.3%, whereas overall pregnancy rates for the 45-day breeding season ranged from 81.3% to 100%. When expressed as a percentage of the original heifers purchased from each source, overall pregnancy rates ranged from 5.3% to 80%. Heifers that lost their fetuses were sold for a net loss of $213 per head. Heifers sold as first service AI bred, second service AI bred, and naturally mated netted$160, $129, and$89 per head, respectively. With accurate records, stringent culling practices, and evaluation of cost and performance, producers can optimize profit potential of replacement heifers. Early culling and pregnancy diagnosis also will decrease costs while increasing opportunities to minimize the financial risks

A novel estrus-synchronization program for anestrous and cycling, suckled, beef cows

We used four herds at three Kansas ranches to evaluate the potential of two new estrus synchronization strategies to increase estrus expression and fertility of 911 crossbred suckled beef cows. The treatments included: 1) 100 μg of GnRH and a 6-mg norgestomet ear implant on day -7 and 25 mg of PG F2" and implant removal on day 0 (GnRH+NORG+PG F2"); 2) 100 μg of GnRH on day - 7 and 25 mg of PGF 2" on day 0 (GnRH+PG F2"); and 3 ) (control) 25- mg injections of PG F2" on days -14 and 0; (2×PGF2" control) . The GnRH+NORG+ PGF 2" and GnRH+PGF treatments increased (P<.01) 2" the overall percentages of cows detected in estrus by 49% and 27% and pregnancy rates by 46% and 37%, respectively, over the control group, without altering conception rate. Both treatments increase d the estrus, conception, and pregnancy rates of noncycling cows, compared to controls

Site of semen deposition and fertility in lactating beef cows synchronized with GnRH and PGF2α

Our objective was to determine the effect of site of semen deposition on pregnancy rate in beef cows inseminated at a fixed time or after observed estrus. Cows were synchronized with a combination of gonadotropin-releasing hormone (GnRH) prostaglandin-F α (PGF). GnRH was injected 7 days before PGF (day 0; first of breeding season). The trial was conducted at two locations, one in Kansas (147 cows) and one in Colorado (313 cows). At each location, cows were assigned to be inseminated after observed estrus (ESTRUS-AI) or at a fixed time (TIMED-AI). Within these two groups, cows either were inseminated in the uterine body (BODY-bred) or in both uterine horns (HORN-bred). Cows in the ESTRUS-AI group were observed for estrus each morning and evening until day 5 afterPGF and then inseminated 12 hr after first detected estrus. Cows in the TIMED-AI group received a second dose of GnRH on day 2 and were inseminated at that time (48 to 56 hr after PGF). Heat response, AI conception rate, and pregnancy rate were analyzed for BODY-bred and HORN-bred cows within each treatment at each location. No differences in these variables occurred between locations, so the results were combined. Within the ESTRUS-AI group, neither conception rate (70% vs. 73%) nor pregnancy rate (39% vs. 40%) was different between BODY-bred and HORN-bred cows respectively. Pregnancy rate within the TIMED-AI group tended (P=.09) to be greater for BODY-bred (53%) compared to HORN-bred (42%) cows. When BODY-bred and HORN-bred treatments were combined, the pregnancy rate of TIMED-AI cows (48%) tended (P=.07) to be greater than that of ESTRUS-AI cows (39%). Timed-insemination resulted in a greater pregnancy rate than inseminating cows according to estrus. No advantage was seen in conception rates when semen was deposited in the uterine horns compared to the uterine body

Phenotypic relationships between docility and reproduction in Angus heifers

Citation: White, K. L., Bormann, J. M., Olson, K. C., Jaeger, J. R., Johnson, S., Downey, B., . . . Weaber, R. L. (2016). Phenotypic relationships between docility and reproduction in Angus heifers. Journal of Animal Science, 94(2), 483-489. doi:10.2527/jas2015-9327The objective of this study was to elucidate the phenotypic relationships between docility and first-service AI conception rate in heifers. Data (n = 337) collected from 3 cooperator herds in Kansas at the start of synchronization protocol included exit velocity (EV), chute score (CS), fecal cortisol (FC), and blood serum cortisol (BC). Data were analyzed using logistic regression with 30-d pregnancy rate as the dependent variable. The model included the fixed effect of contemporary group and the covariates FC, BC, EV, CS, BW, and age. Correlation coefficients were calculated between all continuous traits. Pregnancy rate ranged from 34% to 60% between herds. Blood cortisol positively correlated with EV (r = 0.22, P < 0.01), negatively correlated with age (r = -0.12, P < 0.03), and tended to be negatively correlated with BW (r = -0.10, P = 0.09). Exit velocity was positively correlated with CS (r = 0.24, P < 0.01) and negatively correlated with BW (r = -0.15, P < 0.01) and age (r = -0.12, P < 0.03). Chute score negatively correlated with age (r = -0.14, P < 0.01), and age and BW were moderately positively correlated (r = 0.42, P < 0.01), as expected. Older, heavier animals generally had better temperament, as indicated by lower BC, EV, and CS. The power of our test could detect no significant predictors of 30-d pregnancy for the combined data from all ranches. When the data were divided by ranch, CS (P < 0.03) and BW (P < 0.01) were both significant predictors for 30-d pregnancy for ranch 1. The odds ratio estimate for CS has an inverse relationship with pregnancy, meaning that a 1-unit increase in average CS will reduce the probability of pregnancy at ranch 1 by 48.1%. Weight also has a negative impact on pregnancy because a 1-kg increase in BW will decrease the probability of pregnancy by 2.2%. Fertility is a complex trait that depends on many factors; our data suggest that docility is 1 factor that warrants further investigation

Effect of growth-promoting technologies on Longissimus lumborum muscle fiber morphometrics, collagen solubility, and cooked meat tenderness

Citation: Ebarb, S. M., Drouillard, J. S., Maddock-Carlin, K. R., Phelps, K. J., Vaughn, M. A., Burnett, D. D., . . . Gonzalez, J. M. (2016). Effect of growth-promoting technologies on Longissimus lumborum muscle fiber morphometrics, collagen solubility, and cooked meat tenderness. Journal of Animal Science, 94(2), 869-881. doi:10.2527/jas2015-9888The objective of the study was to examine the effect of growth-promoting technologies (GP) on Longissimus lumborum steak tenderness, muscle fiber cross-sectional area (CSA), and collagen solubility. Crossbred feedlot heifers (n = 33; initial BW 464 +/- 6 kg) were blocked by BW and assigned to 1 of 3 treatments: no GP (CON; n = 11); implant, no zilpaterol hydrochloride (IMP; n = 11); implant and zilpaterol hydrochloride (COMBO; n = 11). Heifers assigned to receive an implant were administered Component TE-200 on d 0 of the study, and the COMBO group received 8.3 mg/kg DM of zilpaterol hydrochloride for the final 21 d of feeding with a 3 d withdrawal period. Following harvest, strip loins were collected and fabricated into 4 roasts and aged for 3, 14, 21, or 35 d postmortem. Fiber type was determined by immunohistochemistry. After aging, objective tenderness and collagen solubility were measured. There was a treatment x day of aging (DOA) interaction for Warner-Bratzler shear force (WBSF; P 0.31). Soluble collagen amount tended to be affected (P = 0.06) by a treatment x DOA interaction which was due to COMBO muscle having more soluble collagen than the other 2 treatments on d 21 of aging (P < 0.02). Correlation analysis indicated that type I, IIA, and IIX fiber CSA are positively correlated with WBSF at d 3 and 14 of aging (P < 0.01), but only type IIX fibers are correlated at d 21 and 35 of aging (P < 0.03). At these time periods, total and insoluble collagen became positively correlated with WBSF (P < 0.01). This would indicate that relationship between muscle fiber CSA and WBSF decreases during postmortem aging, while the association between WBSF and collagen characteristics strengthens. The use of GP negatively impacted meat tenderness primarily through increased muscle fiber CSA and not through altering collagen solubility

Effects of anabolic implants and ractopamine-HCl on muscle fiber morphometrics, collagen solubility, and tenderness of beef longissimus lumborum steaks

Citation: Ebarb, S. M., Phelps, K. J., Drouillard, J. S., Maddock-Carlin, K. R., Vaughn, M. A., Burnett, D. D., . . . Gonzalez, J. M. (2017). Effects of anabolic implants and ractopamine-HCl on muscle fiber morphometrics, collagen solubility, and tenderness of beef longissimus lumborum steaks. Journal of Animal Science, 95(3), 1219-1231. doi:10.2527/jas2016.1263The objective of this study was to examine the effects of growth-promoting technologies (GP) and postmortem aging on longissimus lumborum muscle fiber cross-sectional area (CSA), collagen solubility, and their relationship to meat tenderness. Two groups of black-hided crossbred feedlot heifers (group 1: n = 33, initial BW 430 +/- 7 kg; group 2: n = 32, initial BW 466 +/- 7 kg) were blocked by BW and assigned to 1 of 3 treatments consisting of: no implant and no ractopamine hydrochloride (CON; n = 21); implant, no ractopamine hydrochloride (IMP; n = 22); implant and ractopamine hydrochloride (COMBO; n = 22). Heifers that received an implant were administered an implant containing 200 mg trenbolone acetate and 20 mg estradiol on d 0 of the study, and heifers in the COMBO group received 400 mg.head(-1).d(-1) of ractopamine hydrochloride for 28 (Group 1) or 29 d (Group 2) at the end of 90-(Group 1) or 106-d (Group 2) feeding period. Following harvest, strip loins were collected and further fabricated into 5 roasts for postmortem aging (DOA) periods of 2, 7, 14, 21, or 35 d. After aging, Warner-Bratzler shear force (WBSF), muscle fiber CSA, and collagen solubility were measured. There was no treatment x DOA interaction for WBSF (P = 0.86), but treatment and DOA impacted WBSF (P 0.33). Collagen amounts were not impacted by GP treatment (P > 0.72), but DOA increased the concentration of soluble collagen (P = 0.04). Fiber CSA of all fiber types were positively correlated (P < 0.05; r = 0.21 to 0.28) with WBSF only on d 2 of aging, while soluble collagen amount tended to negatively correlate with WBSF on d 7 and 14 of aging (P < 0.10; r = -0.24 and -0.23, respectively). Administration of GP during heifer finishing resulted in greater steak WBSF over 35 d of aging, which was not due to collagen characteristics and only minimally affected by fiber CSA

Gonadotropin-releasing hormone increased pregnancy risk in suckled beef cows not detected in estrus and subjected to a split-time artificial insemination program

Citation: Hill, S. L., Grieger, D. M., Olson, K. C., Jaeger, J. R., Dahlen, C. R., Crosswhite, M. R., . . . Stevenson, J. S. (2016). Gonadotropin-releasing hormone increased pregnancy risk in suckled beef cows not detected in estrus and subjected to a split-time artificial insemination program. Journal of Animal Science, 94(9), 3722-3728. doi:10.2527/jas2016-0582We hypothesized that GnRH would increase pregnancy risk (PR) in a split-time AI program for cows in which estrus was not detected. A total of 1,236 suckled beef cows at 12 locations in 3 states (Colorado, Kansas, and North Dakota) were enrolled. Before applying the fixed-time AI program, BCS was assessed. Cows were treated on d -7 with a progesterone insert concurrent with 100 mu g GnRH and on d 0 with 25 mg PGF(2 alpha) plus removal of the insert. Estrus-detection patches were affixed to cows at insert removal. Estrus was defined to have occurred when an estrus-detection patch was >50% colored (activated). Cows in estrus by 65 h (n = 758; 61.3% of all cows) were randomly allocated to 2 treatments: 1) 100 mu g GnRH and early + GnRH (E+G; n = 373) or 2) AI only at 65 h (early -no GnRH [E-G]; n = 385). The remaining cows were randomly allocated to 2 treatments: 1) 5(L+G; n = 252) or 2) AI only at 84 h (late no GnRH [L-G]; n = 226). Pregnancy was determined 35 d after AI via transrectal ultrasound. Pregnancy risk did not differ (P = 0.68) between E+G and E-G cows (61.9 vs. 60.4%, respectively). Conversely, for cows inseminated at 84 h, PR was greater (P = 0.01) in cows that received GnRH (L+G) compared with their herd mates not receiving GnRH (L-G; 41.7 vs. 30.8%, respectively). Of those cows not detected in estrus by 65 h, 42.1% were detected by 84 h, for a total expression of estrus by all cows of 77.6%. Administration of GnRH increased (P < 0.01) PR in cows not detected in estrus by 84 h (+ GnRH = 33.4% [n = 146] vs. no GnRH = 15.0% [n = 128]) but had no effect in cows expressing estrus by 84 h (+ GnRH = 65.3% [n = 103] vs. no GnRH = 61.7% [n = 97]). Neither estrus expression by 65 or 84 h nor PR was influenced by BCS, parity, or days postpartum at AI. Cows had greater PR when they had been detected in estrus before AI, and PR was improved by administration of GnRH at 65 h after insert removal in cows that were not detected in estrus and inseminated at 84 h

Using estrus detection patches to optimally time insemination improved pregnancy risk in suckled beef cows enrolled in a fixed-time artificial insemination program

Citation: Hill, S. L., Grieger, D. M., Olson, K. C., Jaeger, J. R., Dahlen, C. R., Bridges, G. A., . . . Stevenson, J. S. (2016). Using estrus detection patches to optimally time insemination improved pregnancy risk in suckled beef cows enrolled in a fixed-time artificial insemination program. Journal of Animal Science, 94(9), 3703-3710. doi:10.2527/jas2016-0469A multilocation study examined pregnancy risk (PR) after delaying AI in suckled beef cows from 60 to 75 h when estrus had not been detected by 60 h in response to a 7-d CO-Synch + progesterone insert (CIDR) timed AI (TAI) program (d-7: CIDR insert concurrent with an injection of GnRH; d 0: PGF(2 alpha) injection and removal of CIDR insert; and GnRH injection at TAI [ 60 or 75 h after CIDR removal]). A total of 1,611 suckled beef cows at 15 locations in 9 states (CO, IL, KS, MN, MS, MT, ND, SD, and VA) were enrolled. Before applying the fixed-time AI program, BCS was assessed, and blood samples were collected. Estrus was defined to have occurred when an estrus detection patch was >50% colored (activated). Pregnancy was determined 35 d after AI via transrectal ultrasound. Cows (n = 746) detected in estrus by 60 h (46.3%) after CIDR removal were inseminated and treated with GnRH at AI (Control). Remaining nonestrous cows were allocated within location to 3 treatments on the basis of parity and days postpartum: 1) GnRH injection and AI at 60 h (early-early = EE; n = 292), 2) GnRH injection at 60 h and AI at 75 h (early-delayed = ED; n = 282), or 3) GnRH injection and AI at 75 h (delayed-delayed = DD; n = 291). Control cows had a greater (P < 0.01) PR (64.2%) than other treatments (EE = 41.7%, ED = 52.8%, DD = 50.0%). Use of estrus detection patches to delay AI in cows not in estrus by 60 h after CIDR insert removal (ED and DD treatments) increased (P < 0.05) PR to TAI when compared with cows in the EE treatment. More (P < 0.001) cows that showed estrus by 60 h conceived to AI at 60 h than those not showing estrus (64.2% vs. 48.1%). Approximately half (49.2%) of the cows not in estrus by 60 h had activated patches by 75 h, resulting in a greater (P < 0.05) PR than their nonestrous herd mates in the EE (46.1% vs. 34.5%), ED (64.2% vs. 39.2%), and DD (64.8% vs. 31.5%) treatments, respectively. Overall, cows showing estrus by 75 h (72.7%) had greater (P < 0.001) PR to AI (61.3% vs. 37.9%) than cows not showing estrus. Use of estrus detection patches to allow for a delayed AI in cows not in estrus by 60 h after removal of the CIDR insert improved PR to TAI by optimizing the timing of the AI in those cows

Earth Virtualization Engines (EVE)

To manage Earth in the Anthropocene, new tools, new institutions, and new forms of international cooperation will be required. Earth Virtualization Engines is proposed as an international federation of centers of excellence to empower all people to respond to the immense and urgent challenges posed by climate change