Templated mineralization by charge-modified cowpea mosaic virus

Templated mineralization of virus particles provides routes to narrowly dispersed nanoparticles that are not readily prepared by other means. The templated mineralization of metal or metal oxide on the external surface of wild-type cowpea mosaic virus (CPMV), a plant virus, is facilitated by increasing the external surface negative charge. This is achieved by the chemical modification of surface lysine groups by succinic anhydride. Hence, for example, treatment of charge-modified CPMV succinamate with a 1:2 mixture of iron(II) and iron(III) salts, followed by raising the pH to 10.2, led to the formation of narrowly dispersed, CPMV-templated, magnetite (Fe3O4) nanoparticles

Polyelectrolyte-modified cowpea mosaic virus for the synthesis of gold nanoparticles

Polyelectrolyte surface-modified cowpea mosaic virus (CPMV) can be used for the templated synthesis of narrowly dispersed gold nanoparticles. Cationic polyelectrolyte, poly(allylamine) hydrochloride, is electrostatically bound to the external surface of the virus capsid. The polyelectrolyte-coated CPMV promotes adsorption of aqueous gold hydroxide anionic species, prepared from gold(III) chloride and potassium carbonate, that are easily reduced to form CPMV-templated gold nanoparticles. The process is simple and environmentally benign using only water as solvent at ambient temperature

Toward Full LHC Coverage of Natural Supersymmetry

We argue that combining just a handful of searches for new physics at Run I of the LHC is sufficient to exclude most supersymmetric extensions of the Standard Model in which the gluino is kinematically accessible and the spectrum is natural. Such models typically give rise to significant MET, top quarks and/or high object multiplicity, and we show that having even one of these signatures generally results in stringent limits. We also identify, among models that lack these signatures, the few gaps in coverage remaining, and propose search strategies to close these gaps. Our results are general and independent of the details of the spectrum, assumptions about minimality, R-parity, etc. Our analysis strategy should remain applicable when the LHC moves to higher energy. Central to our argument are ATLAS and CMS searches for many jets and low MET, a proposed lepton + many jets search, an ATLAS search for 6-7 high-pT jets, and a reexamination of the control and signal regions of the CMS black hole search.Comment: 53 pages, 16 figures, journal versio

A Direct Upper Limit on the Density of Cosmological Dust from the Absence of an X-ray Scattering Halo around the z=4.3 QSO 1508+5714

We report on the results of a search for an intergalactic X-ray dust scattering halo in a deep observation of the bright, high-redshift quasar QSO 1508+5714 with the Chandra X-ray Observatory. We do not detect such a halo. Our result implies an upper limit on the density of diffuse, large-grained intergalactic dust of Omega_ dust < 2 x 10^-6, assuming a characteristic grain size of 1micron. The result demonstrates the sensitivity of this technique for detecting very small amounts of intergalactic dust which are very hard to detect otherwise. This will allow us to put important constraints on systematic effects induced by extinction on the interpretation of the SN Ia Hubble Diagram, as well as on the amount and properties of cosmological dust being expelled into the intergalactic medium at early z~2 times.Comment: 14 pages, 2 figures. to appear in ApJ, vol. 651, Nov. 200

The Unfinished Business of US Drug Safety Regulation

Posted with permission from FDLI ; Food and Drug Law Journal Various proposals have been advanced in response to recent problems with the safety of Food and Drug Administration (FDA)-approved drugs. Many call for incremental change, such as new safety oversight bodies or minor expansions of FDA's existing powers. "Fixing" FDA may not fix the problem, without related reform of the broader legal framework in which FDA operates. Key reform challenges include promoting clinical compliance with important safety warnings while preserving needed flexibility for physicians to adapt drug use to the individual patient; developing a clearer distinction between pre- and postapproval safety regulation; and devising mechanisms for funding investments in safety improvements. Until these fundamental problems are addressed, the United States will face ongoing problems with drug safety and patients will be denied the full measure of safety and therapeutic benefit that today's technologies could support. This article proposes a new direction to address these problems in the context of an insurance-based framework for promoting drug safety

3D quantitative imaging of unprocessed live tissue reveals epithelial defense against bacterial adhesion and subsequent traversal requires MyD88.

While a plethora of in vivo models exist for studying infectious disease and its resolution, few enable factors involved in the maintenance of health to be studied in situ. This is due in part to a paucity of tools for studying subtleties of bacterial-host interactions at a cellular level within live organs or tissues, requiring investigators to rely on overt outcomes (e.g. pathology) in their research. Here, a suite of imaging technologies were combined to enable 3D and temporal subcellular localization and quantification of bacterial distribution within the murine cornea without the need for tissue processing or dissection. These methods were then used to demonstrate the importance of MyD88, a central adaptor protein for Toll-Like Receptor (TLR) mediated signaling, in protecting a multilayered epithelium against both adhesion and traversal by the opportunistic bacterial pathogen Pseudomonas aeruginosa ex vivo and in vivo

Spectral Measures and Generating Series for Nimrep Graphs in Subfactor Theory II: SU(3)

We complete the computation of spectral measures for SU(3) nimrep graphs arising in subfactor theory, namely the SU(3) ADE graphs associated with SU(3) modular invariants and the McKay graphs of finite subgroups of SU(3). For the SU(2) graphs the spectral measures distill onto very special subsets of the semicircle/circle, whilst for the SU(3) graphs the spectral measures distill onto very special subsets of the discoid/torus. The theory of nimreps allows us to compute these measures precisely. We have previously determined spectral measures for some nimrep graphs arising in subfactor theory, particularly those associated with all SU(2) modular invariants, all subgroups of SU(2), the torus, SU(3), and some SU(3) graphs.Comment: 38 pages, 21 figure

Membrane geometry with auxiliary variables and quadratic constraints

Consider a surface described by a Hamiltonian which depends only on the metric and extrinsic curvature induced on the surface. The metric and the curvature, along with the basis vectors which connect them to the embedding functions defining the surface, are introduced as auxiliary variables by adding appropriate constraints, all of them quadratic. The response of the Hamiltonian to a deformation in each of the variables is examined and the relationship between the multipliers implementing the constraints and the conserved stress tensor of the theory established.Comment: 8 page

Validation and Improvement of the Beef Production Sub-index in Ireland for Beef Cattle

End of project reportThe objectives of the following study were to: a. Quantify the effect of sire genetic merit for BCI on: 1. feed intake, growth and carcass traits of progeny managed under bull or steer beef production systems. 2. live animal scores, carcass composition and plasma hormone and metabolite concentrations in their progeny. b. Compare the progeny of : 1. Late-maturing beef with dairy breeds and 2. Charolais (CH), Limousin (LM), Simmental (SM) and Belgian Blue (BB) sires bred to beef suckler dams, for feed intake, blood hormones and metabolites, live animal measurements, carcass traits and carcass value in bull and steer production systems

Perpetual Protection for Atlanta’s High-Quality Forested Land in the City

Unlike most major U.S. cities, developed and industrialized decades earlier, Atlanta retains a large portion of its native and originally forested land with a high diversity of species, rare plants, and even old-growth trees. A 2008 baseline canopy analysis found that while the city’s tree canopy cover was among the highest in the country (47.9%), its canopy and high-quality forests were vulnerable to loss and fragmentation since only 4.9% of the canopy was on public land. In 2016, the city authorized the use of its Tree Trust Fund to purchase high-quality forested land for perpetual protection and established criteria for evaluating, prioritizing, and selecting these natural areas for purchase. The first acquisition occurred in 2020, resulting in the protection of Lake Charlotte Nature Preserve, a 216-acre oak-hickory forest, one of the largest remaining mature forests in the city, which was under major threat of industrial development. This case study discusses this innovative funding mechanism and the selection criteria for identifying high-quality urban forests