5'-terminal structure of poliovirus polyribosomal RNA is pUp

Poliovirus RNA purified from virus-specific polyribosomes does not contain m7G in a 5'-5'-pyrophosphate linkage at its 5'-end. The only potential 5'-end found in ribonuclease digests of this RNA is pUp, which is present in a yield of 1 mole/mole of poliovirus RNA. We conclude that a 5'-terminal m7G is not required for translation of at least one RNA species in animal cells

Substitution and representation: patterns of thought in Christian atonement theology

This thesis examines the use of the terms Substitution and Representation as they are found to occur in Christian Atonement Theology. It is a theological investigation into the use of the terms, in order to establish their meaning and the relation between them. Both terms are first subjected to a phenomenological enquiry in order to disclose the various patterns of thought in which the terms function and which they help to generate. The part played by the terms within these different contexts, together with the models they espouse, are then critically analysed and evaluated. In this way the full complexity of the terms Substitution and Representation is examined, the appropriateness of their range of meanings tested, and the part they play in Christian Atonement Theology determined. To test the adequacy and accuracy of this analysis a more detailed study is made of the work of D. Sölle, Christ the Representative. Her place within the continuing debate surrounding the terms is established, and more light is shed on the use and meaning of the terms Substitution and Representation

Alfalfa : An Economic Alternative to Corn?

Production costs and returns of alfalfa for different levels of management and at different prices are compared to shelled corn and corn silage, the major crops grown on the Belle Fourche Irrigation District

An Investigation of the Acute Effects of Oligofructose-Enriched Inulin on Subjective Wellbeing, Mood and Cognitive Performance

Acknowledgments: The research described here was supported by a grant from Orafti, BelgiumPeer reviewedPublisher PD

The Medecins Sans Frontieres Intervention in the Marburg Hemorrhagic Fever Epidemic, Uige, Angola, 2005. I. Lessons Learned in the Hospital.

When the epidemic of Marburg hemorrhagic fever occurred in Uige, Angola, during 2005, the international response included systems of case detection and isolation, community education, the burial of the dead, and disinfection. However, despite large investments of staff and money by the organizations involved, only a fraction of the reported number of cases were isolated, and many cases were detected only after death. This article describes the response of Medecins Sans Frontieres Spain within the provincial hospital in Uige, as well as the lessons they learned during the epidemic. Diagnosis, management of patients, and infection control activities in the hospital are discussed. To improve the acceptability of the response to the host community, psychological and cultural factors need to be considered at all stages of planning and implementation in the isolation ward. More interventional medical care may not only improve survival but also improve acceptability

MST construction in O

We consider a simple model for overlay networks, where all n processes are connected to all other processes, and each message contains at most O(log n) bits. For this model, we present a distributed algorithm that constructs a minimumweight spanning tree in O(log log n) communication rounds, where in each round any process can send a message to each other process. This result is the first to break the Ω(log n) parallel time complexity barrier with small message sizes. Categories and Subject Descriptors F.2 [theory of computation]: analysis of algorithms and problem complexity; G.2.2 [mathematics of computing]: discrete mathematics—graph theor

A Hybrid Sequencing Approach Completes the Genome Sequence of Thermoanaerobacter ethanolicus JW 200

Thermoanaerobacter ethanolicus JW 200 has been identified as a potential sustainable biofuel producer due to its ability to readily ferment carbohydrates to ethanol. A hybrid sequencing approach, combining Oxford Nanopore and Illumina DNA sequence reads, was applied to produce a single contiguous genome sequence of 2,911,280 bp

Temperature-Dependence of Weibel-Palade Body Exocytosis and Cell Surface Dispersal of von Willebrand Factor and Its Propolypeptide

Background: Weibel-Palade bodies (WPB) are endothelial cell (EC) specific secretory organelles containing Von Willebrand factor (VWF). The temperature-dependence of Ca2+-driven WPB exocytosis is not known, although indirect evidence suggests that WPB exocytosis may occur at very low temperatures. Here we quantitatively analyse the temperature-dependence of Ca2+-driven WPB exocytosis and release of secreted VWF from the cell surface of ECs using fluorescence microscopy of cultured human ECs containing fluorescent WPBs. Principal Findings: Ca2+-driven WPB exocytosis occurred at all temperatures studied (7–37°C). The kinetics and extent of WPB exocytosis were strongly temperature-dependent: Delays in exocytosis increased from 0.92 s at 37°C to 134.2 s at 7°C, the maximum rate of WPB fusion decreased from 10.0±2.2 s−1 (37°C) to 0.80±0.14 s−1 (7°C) and the fractional extent of degranulation of WPBs in each cell from 67±3% (37°C) to 3.6±1.3% (7°C). A discrepancy was found between the reduction in Ca2+-driven VWF secretion and WPB exocytosis at reduced temperature; at 17°C VWF secretion was reduced by 95% but WPB exocytosis by 75–80%. This discrepancy arises because VWF dispersal from sites of WPB exocytosis is largely prevented at low temperature. In contrast VWF-propolypeptide (proregion) dispersal from WPBs, although slowed, was complete within 60–120 s. Novel antibodies to the cleaved and processed proregion were characterised and used to show that secreted proregion more accurately reports the secretion of WPBs at sub-physiological temperatures than assay of VWF itself. Conclusions : We report the first quantitative analysis of the temperature-dependence of WPB exocytosis. We provide evidence; by comparison of biochemical data for VWF or proregion secretion with direct analysis of WPB exocytosis at reduced temperature, that proregion is a more reliable marker for WPB exocytosis at reduced temperature, where VWF-EC adhesion is increased

Utility of Repeat Testing for COVID-19: Laboratory Stewardship When the Stakes are High

As the coronavirus disease 2019 (COVID-19) continues to circulate, testing strategies are of the utmost importance. Given national shortages of testing supplies, personal protective equipment, and other hospital resources, diagnostic stewardship is necessary to aid in resource management. We report the low utility of serial testing in a low-prevalence setting

Global predicate analysis and its application to register allocation

Abstract To fully utilize the wide machine resources in modern high-performance microprocessors it is necessary to exploit parallelism beyond individual basic blocks. Architectural support for predicated execution increases the degree of instruction level parallelism by allowing instructions from dtgerent basic blocks to be converted to straight-line code guarded by boolean predicates. However; predicated execution also presents signijcant challenges to an optimizing compiler For example, in live range analysis, a predicated definition does not necessarily end the live range of a virtual register This paper describes techniques to analyze the relations among predicates in order to improve the precision and effectiveness of various compiler analysis and transformation phases in the presence of predicated code. Our predicate analysis operates globally to obtain relations among predicates. Moreover we analyze control flow and predication in a single unifiedframework. The result can be queried by subsequent optimization and analysis phases. Based on this framework, we extend a traditional method to a predicate-aware register allocator which takes global predicate relations into account. We have implemented the proposed algorithms to effectively reduce register pressure. Our experimental results show 24.6% of a large test suite obtain, on average, 20.71% better register allocation due to the algorithms presented in this paper