Synthetic phospholipid vesicles containing a purified viral antigen and cell membrane proteins stimulate the development of cytotoxic T lymphocytes

Synthetic phospholipid vesicles (liposomes) containing the purified glycoprotein (G) of vesicular stomatitis virus (VSV) and solubilized membrane proteins from cells of the appropriate H-2 haplotype elicited H-2-restricted cytotoxic T lymphocytes (CTL) that lysed VSV-infected target cells. The CTL were elicited by intact liposomes, not by released components. Thus, when spleen cells from VSV-primed H-2d X H- 2b hybrid mice were stimulated with liposomes having G protein + membrane proteins from cells with one of the parental H-2 haplotypes, the resulting CTL lysed only VSV-infected target cells with that parent's H-2 type. This result argues against the view that T cells in general recognize only processed antigenic fragments on macrophages. Moreover, liposomes were only effective when G protein and cell membrane proteins were included in the same vesicles. This result suggests that for effective interaction with CTL precursors the antigen (G protein) and products of the H-2 complex must be closer to each other than 600-1,000 angstrom, the diameter of the lipid vesicles used in this study

Vesicular stomatitis virus glycoprotein is necessary for H-2-restricted lysis of infected cells by cytotoxic T lymphocytes

Vesicular stomatitis virus (VSV) elicited cytotoxic thymus-derived lymphocytes (CTLs) in mice of the BALB/c and three congenic strains (BALB.b, BALB.k, BALB.HTG). CTL lysis of VSV-infected fibroblasts from the four strains was restricted by the target cells' major histocompatibility complex (H-2). Target cells were also infected with two temperature-sensitive mutants of VSV, tsM and tsG in which, respectively, the viral matrix protein and glycoprotein are not expressed at 39 degrees (restrictive temperature) on the infected cell's surface membrane. At the restrictive temperature, cells infected with wild-type VSV or tsM were lysed by CTLs, but cells infected with tsG were not. The requirement for the glycoprotein on the target cell was also evident from the ability of antisera to the glycoprotein to block completely CTL lysis of VSV-infected cells

A water rights transfer evaluation procedure with applications for western energy development

This report deals with questions of water supply for coal deve- lopment in the semiarid western United States. A method is developed to evaluate yields of water rights in "appropriation" or "permit" systems of water administration. Water rights are characterized in terms of location, priority, decreed maximum diversion, actual diver- sion in periods of low flow, and consumptive use. Transfers of water rights are evaluated in this method by using institutional procedures as a framework for analysis. A case study is performed on the North Fork of the Powder River, Wyoming, in which institutional considera- tions are discussed, and water rights are evaluated for a hypothetical facility. This procedure is not limited to energy facilities, but may be used in most cases of water rights trasnfers. The method is designed for use with easily obtained data in order to facilitate its use in practice.Sponsored by U.S. Dept. of Energy

2-Thiabicyclo[3.2.0]hepta-3,6-Dienes. 1. Aluminum Chloride Catalyzed Thermal [2 + 2] Cycloaddition of 2-Butynedinitrile with Alkyl-Substituted Thiophenes

Tetra- and Trialkylthiophenes 4–6 React with 2-Butynedinitrile in the Presence of 1 Equiv of Aluminum Chloride in Dichloromethane at 0 °C Via [2 + 2] Cycloaddition to 2-3.2.0] hepta-3,6-Diene-6,7-Dicarbonitriles 7–10 as the Major Products. Reaction of 4 and 5 Also Gave Small Amounts (4% and 6%) of 1,2-Benzenedicarbonitriles 11 and 12 Which Result from [4 + 2] Cycloaddition and Subsequent Extrusion of Sulfur. 4,5,6,7-Tetrahydro-1,3-Dimethylbenzo[C]thiophene (6) Reacts with 2-Butynedinitrile under the Same Conditions to Give an Ene Adduct (13) in Addition to the [2 + 2] Cycloadduct 10. 2,5-Dimethylthiophene (3) Gave a 2:1 Reaction Product 14, the Formation of Which Can Be Explained Via Friedel-Crafts Alkylation of 3 by the Initially Formed [2 + 2] Cycloadduct. the 2-3.2.0] hepta-3,6-Dienes 7–10 and the Ene Adduct 13 Show Intramolecular Charge-Transfer Absorptions in the UV Spectra. © 1982, American Chemical Society. All Rights Reserved

Pelaksanaan Fungsi Aparat Pengawas Intern Pemerintah (Apip) Untuk Menunjang Tingkat Kewajaran Laporan Keuangan Pemerintah Kota Manado

Internal Audit is the whole process of audit activities, the Review, evaluation, monitoring and supervisory activities other against the implementation of tasks and functions of the organization in order to provide reasonable assurance that the activities have been carried out in accordance with the benchmarks that have been set effectively and efficiently for the benefit of the leadership to Realize good governance.Accountability and transparency in the new government could be achieved if all levels of leadership controlling activities on the overall activities in their respective agencies . Internal control system ( SPI ) is a process that is integral to the actions and activities carried out continuously by the management and all employees to provide reasonable assurance on the achievement of organizational goals through effective and efficient , the reliability of financial reporting , the safeguarding of state assets, and compliance with legislation. To strengthen and support the effectiveness of internal control over the SPI made ​​implementation of tasks and functions of government agencies including state audits carried out by the internal control official Government ( APIP ) .The auditor\u27s responsibility to detect fraud or irregularity embodied in the planning and execution to obtain reasonable assurance about whether the financial statements have been prepared in accordance with established standards.The purpose of this study was to analyze the functions of Government Internal Supervisory Apparatus (APIP) / Internal Auditor in supporting reasonable levels of Local Government Finance Report Manado City. Respondents were selected are few skilled examiner at the office of the Inspectorate of Manado. Approach Using Qualitative methods Ethnography.APIP functions that work well to prevent fraud , to produce valuable output to be input to the external auditor , the executive and the legislature to improve financial management and accountability in the area of ​​the future . Eksternal Auditor may use the results from the monitoring APIP mainly reviews the financial statements of the government , supporting the management of local government in implementing the recommendations and improvements Internal control system . APIP professional and independent encourage increased transparency and accountability in financial management to improve the fairness of the financial statements

2-Thiabicyclo[3.2.0]-hepta-3,6-dienes. 2. Thermal and photochemical isomerization of 2-thiabicyclo[3.2.0]hepta-3,6-dienes. An example of the antarafacial-antarafacial Cope rearrangement

Thermal Rearrangement of 2-3.2.0] hepta-3,6-Diene-6,7-Dicarbonitrile8 1–3 to their 4,5-Dicarbonitrile Isomers 4–6 Takes Place at 110–140 °C in Yields of 82–84%. the Reactions Are First Order with Rates Almost Independent of the Polarity of the Solvent. Activation Parameters (ΔH‡ = 120–140 KJ Mol−1 and ΔS‡ ≈ 0 J K−1 Mol−1) Are in Agreement with a Concerted Symmetry-Allowed Antarafacial-Antarafacial Cope Rearrangement. Compounds 1–3 Rearrange Photochemically Via Cleavage of the C-1-S Bond to the 1,7-Dicarbonitrile Isomers 7–9. Isomer 9 Rearranges Further on Prolonged Irradiation to Give 10. a Tentative Mechanism for This Rearrangement is Given. © 1982, American Chemical Society. All Rights Reserved

Analisis Kesiapan Pemerintah Daerah Dalam Menerapkan Standar Akuntansi Pemerintahan Berbasis Akrual Dan Dampaknya Terhadap Sumber Daya Manusia Pada Pemerintah Kota Bitung

This study aims to determine the readiness of the government of Bitung in implementing accrual-based government accounting standards. Data was collected by observation at every SKPD in Bitung City Government followed in-depth interviews with key informants. The data collected was analyzed by qualitative methods. Then based on interviews (Focus Group Discussion) drawn conclusions indicate that Bitung City Government was ready to apply Accrual Based Government Accounting Standards. This is supported by the Government regulations which require to all local governments, including the city of Bitung in applying Accrual Based Government Accounting Standards, as well as Bitung City Government has issued the relevant mayor Accounting Policies in Bitung. Other things that support the readiness of the implementation of Accrual Based Accounting Standards, namely; development of human resources employee, committed leader who supports the changes later infromasi facilities and infrastructure systems are adequate and can accommodate Accrual Based Accounting Standards

Control and Function of Arm Swing in Human Walking and Running

We investigated the control and function of arm swing in human walking and running to test the hypothesis that the arms act as passive mass dampers powered by movement of the lower body, rather than being actively driven by the shoulder muscles. We measured locomotor cost, deltoid muscle activity and kinematics in 10 healthy adult subjects while walking and running on a treadmill in three experimental conditions: control; no arms (arms folded across the chest); and arm weights (weights worn at the elbow). Decreasing and increasing the moment of inertia of the upper body in no arms and arm weights conditions, respectively, had corresponding effects on head yaw and on the phase differences between shoulder and pelvis rotation, consistent with the view of arms as mass dampers. Angular acceleration of the shoulders and arm increased with torsion of the trunk and shoulder, respectively, but angular acceleration of the shoulders was not inversely related to angular acceleration of the pelvis or arm. Restricting arm swing in no arms trials had no effect on locomotor cost. Anterior and posterior portions of the deltoid contracted simultaneously rather than firing alternately to drive the arm. These results support a passive arm swing hypothesis for upper body movement during human walking and running, in which the trunk and shoulders act primarily as elastic linkages between the pelvis, shoulder girdle and arms, the arms act as passive mass dampers which reduce torso and head rotation, and upper body movement is primarily powered by lower body movement.AnthropologyHuman Evolutionary Biolog