Case-Building Behavior, Persistence, and Emergence Success of \u3ci\u3ePycnopsyche Guttifer\u3c/i\u3e (Walker) (Trichoptera: Limnephilidae) in Laboratory and \u3ci\u3ein situ\u3c/i\u3e Environments: Potential Trade-Offs of Material Preference

When removed from their cases in a non-flow laboratory environment, 5th instar Pycnopsyche guttifer (Walker) larvae were always successful in constructing a new case within 24 h when woody debris was present as a material choice. Most were successful within 1 h. Larvae were never successful at case building in the absence of wood in a non-flow environment. These laboratory-constructed ‘emergency cases’ were flimsy, lacking in shape, and larger than field cases. Laboratory case size, shape, and material preference remained constant after repeated daily evacuations over a series of 10 days. Larvae could be induced to construct a case composed of mineral particles only in the absence of wood and when placed in a laboratory stream with simulated flow conditions, or in situ in a natural stream. The emergence success of P. guttifer specimens induced to build these mineral cases, however, was significantly higher than that of larvae remaining in their field cases or of larvae that built wood cases. This result is likely due to a fungal infection that affected only larvae in wood cases. Our results demonstrate a scenario where a clearly non-preferred case construction material appears to increase survival

Online mechanism design for electric vehicle charging

The rapid increase in the popularity of electric vehicles (EVs) and plug-in hybrid electric vehicles (PHEVs) is expected to place a considerable strain on the existing electricity grids, due to the high charging rates these vehicles require. In many places, the limited capacity of the local electricity distribution network will be exceeded if many such vehicles are plugged in and left to charge their batteries simultaneously. Thus, it will become increasingly important to schedule the charging of these vehicles, taking into account the vehicle owners’ preferences, and the local constraints on the network. In this paper, we address this setting using online mechanism design and develop a mechanism that incentivises agents (representing vehicle owners) to truthfully reveal their preferences, as well as when the vehicle is available for charging. Existing related online mechanisms assume that agent preferences can be described by a single parameter. However, this is not appropriate for our setting since agents are interested in acquiring multiple units of electricity and can have different preferences for these units, depending on factors such as their expected travel distance. To this end, we extend the state of the art in online mechanism design to multi-valued domains, where agents have non-increasing marginal valuations for each subsequent unit of electricity. Interestingly, we show that, in these domains, the mechanism occasionally requires leaving electricity unallocated to ensure truthfulness. We formally prove that the proposed mechanism is dominant-strategy incentive compatible, and furthermore, we empirically evaluate our mechanism using data from a real-world trial of electric vehicles in the UK. We show that our approach outperforms any fixed price mechanism in terms of allocation efficiency, while performing only slightly worse than a standard scheduling heuristic, which assumes non-strategic agents

Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle

EB1 is an evolutionarily conserved protein that localizes to the plus ends of growing microtubules. In yeast, the EB1 homologue (BIM1) has been shown to modulate microtubule dynamics and link microtubules to the cortex, but the functions of metazoan EB1 proteins remain unknown. Using a novel preparation of the Drosophila S2 cell line that promotes cell attachment and spreading, we visualized dynamics of single microtubules in real time and found that depletion of EB1 by RNA-mediated inhibition (RNAi) in interphase cells causes a dramatic increase in nondynamic microtubules (neither growing nor shrinking), but does not alter overall microtubule organization. In contrast, several defects in microtubule organization are observed in RNAi-treated mitotic cells, including a drastic reduction in astral microtubules, malformed mitotic spindles, defocused spindle poles, and mispositioning of spindles away from the cell center. Similar phenotypes were observed in mitotic spindles of Drosophila embryos that were microinjected with anti-EB1 antibodies. In addition, live cell imaging of mitosis in Drosophila embryos reveals defective spindle elongation and chromosomal segregation during anaphase after antibody injection. Our results reveal crucial roles for EB1 in mitosis, which we postulate involves its ability to promote the growth and interactions of microtubules within the central spindle and at the cell cortex

Employing paramagnetic shift for responsive MRI probes

Paramagnetic metal ions with anisotropic magnetic susceptibilities can shift the proton NMR signals of chelating ligands beyond the diamagnetic range of endogenous proton resonances. Such large shifts, along with enhanced longitudinal relaxation rates, allow paramagnetic complexes to be exploited as molecular imaging probes for MRI. Paramagnetically-shifted imaging probes are detected directly against zero background, as opposed to the indirect induced relaxation enhancement of surrounding water molecules, and are reviewed herein. The development of ‘smart’ probes that are sensitive to their surrounding environment is also being developed, and some of the challenges faced for in vivo imaging are discussed, including issues of sensitivity and dose, biodistribution and clearance. Several examples of lanthanide complexes have been investigated, and more recently certain paramagnetic transition metal complexes are being considered as potential imaging agents

Roles of motor proteins in building microtubule-based structures: a basic principle of cellular design

AbstractEukaryotic cells must build a complex infrastructure of microtubules (MTs) and associated proteins to carry out a variety of functions. A growing body of evidence indicates that a major function of MT-associated motor proteins is to assemble and maintain this infrastructure. In this context, we examine the mechanisms utilized by motors to construct the arrays of MTs and associated proteins contained within the mitotic spindle, neuronal processes, and ciliary axonemes. We focus on the capacity of motors to drive the ‘sliding filament mechanism’ that is involved in the construction and maintenance of spindles, axons and dendrites, and on a type of particle transport called ‘intraflagellar transport’ which contributes to the assembly and maintenance of axonemes

Mitosis, microtubules, and the matrix

The mechanical events of mitosis depend on the action of microtubules and mitotic motors, but whether these spindle components act alone or in concert with a spindle matrix is an important question

Late-season Insect Pests of Soybean in Louisiana: Preventive Management and Yield Enhancement (Bulletin #880)

The velvetbean caterpillar and the soybean looper are important pests of soybeans in Louisiana. These late-season soybean insect pests create the need for the continuous development of insecticide programs that are cost effective, maintain profitable yields and conserve natural enemies.https://digitalcommons.lsu.edu/agcenter_bulletins/1020/thumbnail.jp

Huntingtin-lowering strategies for Huntington's disease.

INTRODUCTION: Huntington's disease (HD) is an incurable, autosomal dominant neurodegenerative disease caused by an abnormally long polyglutamine tract in the huntingtin protein. Because this mutation causes disease via gain-of-function, lowering huntingtin levels represents a rational therapeutic strategy. AREAS COVERED: We searched MEDLINE, CENTRAL, and other trial databases, and relevant company and HD funding websites for press releases until April 2020 to review strategies for huntingtin lowering, including autophagy and PROTACs, which have been studied in preclinical models. We focussed our analyses on oligonucleotide (ASOs) and miRNA approaches, which have entered or are about to enter clinical trials. EXPERT OPINION: ASO and mRNA approaches for lowering mutant huntingtin protein production and strategies for increasing mutant huntingtin clearance are attractive because they target the cause of disease. However, questions concerning the optimal mode of delivery and associated safety issues remain. It is unclear if the human CNS coverage with intrathecal or intraparenchymal delivery will be sufficient for efficacy. The extent that one must lower mutant huntingtin levels for it to be therapeutic is uncertain and the extent to which CNS lowering of wild-type huntingtin is safe is unclear. Polypharmacy may be an effective approach for ameliorating signs and symptoms and for preventing/delaying onset and progression