Red abalone size data from Johnsons Lee, Santa Rosa Island, collected from 1978 to 1984

Red abalone, Haliotis rufescens, were collected at Johnsons Lee, Santa Rosa Island, in the summers of 1978 through 1982, and in 1984, to obtain data for determining various fishery population parameters. Annual visits to the study site were made at yearly intervals to simplify growth calculations. During the first four visits, 2145 red abalones were tagged, measured, and replaced. Shell damage, soft tissue injuries, and causes of mortality were noted. The method of tagging is described. Recovery of first tagged abalone after one year was approximately 30%. Analysis of variance of the annual samples indicated that the samples were, with one exception, not different. Summaries are presented of the number of abalone collected and tagged by year, frequencies of shell damage, soft tissue injury, predatory sponge infestation, and total mortality. Appendices include a listing of the raw size data and various codes for each tagged abalone. (56pp.

Reverse engineering of pipe layouts and 3D point set damage models

technical reportThis paper focuses on obtaining pipe layout information from depth images, and on the development of damage models for 3D shapes. Techniques are given for recognizing various pipes and pipe features. Methods for generating compact geometric descriptions of pipes and pipe features are discussed. These methods and techniques are analyzed for error, and error measurements are given. 3D shape damage models are proposed based on a separable Gaussian kernel model. Experiments on synthetic and real data were performed and results discussed

Post-conflict mental health in South Sudan: Overview of common psychiatric disorders Part 2: Anxiety and substance abuse

No Abstrac

Allen C. Henderson and David Glaze: A Guest Artist Performance

This is the program for the guest artist concert of Allen C. Henderson and David Glaze, held on September 18, 1995, in Mabee Fine Arts Center\u27s Recital Hall

Post-conflict mental health in South Sudan: overview of common psychiatric disorders Part 1: Depression and post-traumatic stress disorder

No Abstrac

Modification of Coulomb's law in closed spaces

We obtain a modified version of Coulomb's law in two- and three-dimensional closed spaces. We demonstrate that in a closed space the total electric charge must be zero. We also discuss the relation between total charge neutrality of a isotropic and homogenous universe to whether or not its spatial sector is closed.Comment: 11 pages, 3 figure

A Spatio-Temporal Point Process Model for Ambulance Demand

Ambulance demand estimation at fine time and location scales is critical for fleet management and dynamic deployment. We are motivated by the problem of estimating the spatial distribution of ambulance demand in Toronto, Canada, as it changes over discrete 2-hour intervals. This large-scale dataset is sparse at the desired temporal resolutions and exhibits location-specific serial dependence, daily and weekly seasonality. We address these challenges by introducing a novel characterization of time-varying Gaussian mixture models. We fix the mixture component distributions across all time periods to overcome data sparsity and accurately describe Toronto's spatial structure, while representing the complex spatio-temporal dynamics through time-varying mixture weights. We constrain the mixture weights to capture weekly seasonality, and apply a conditionally autoregressive prior on the mixture weights of each component to represent location-specific short-term serial dependence and daily seasonality. While estimation may be performed using a fixed number of mixture components, we also extend to estimate the number of components using birth-and-death Markov chain Monte Carlo. The proposed model is shown to give higher statistical predictive accuracy and to reduce the error in predicting EMS operational performance by as much as two-thirds compared to a typical industry practice

The Genomic and Proteomic Content of Cancer Cell-Derived Exosomes

Exosomes are secreted membrane vesicles that have been proposed as an effective means to detect a variety of disease states, including cancer. The properties of exosomes, including stability in biological fluids, allow for their efficient isolation and make them an ideal vehicle for studies on early disease detection and evaluation. Much data has been collected over recent years regarding the messenger RNA, microRNA, and protein contents of exosomes. In addition, many studies have described the functional role that exosomes play in disease initiation and progression. Tumor cells have been shown to secrete exosomes, often in increased amounts compared to normal cells, and these exosomes can carry the genomic and proteomic signatures characteristic of the tumor cells from which they were derived. While these unique signatures make exosomes ideal for cancer detection, exosomes derived from cancer cells have also been shown to play a functional role in cancer progression. Here, we review the unique genomic and proteomic contents of exosomes originating from cancer cells as well as their functional effects to promote tumor progression

Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts

<p>Abstract</p> <p>Background</p> <p>Protein-based microarray platforms offer considerable promise as high-throughput technologies in proteomics. Particular advantages are provided by self-assembling protein microarrays and much interest centers around analysis of eukaryotic proteins and their molecular interactions. Efficient cell-free protein synthesis is paramount for the production of self-assembling protein microarrays, requiring optimal transcription, translation, and protein folding. The <it>Escherichia coli </it>S30 extract demonstrates high translation rates but lacks the protein-folding efficiency of its eukaryotic counterparts derived from rabbit reticulocyte and wheat germ extract. In comparison to <it>E. coli</it>, eukaryotic extracts, on the other hand, exhibit slower translation rates and poor overall protein yields. A cell-free expression system that synthesizes folded eukaryotic proteins in considerable yields would optimize <it>in vitro </it>translation for protein microarray assembly.</p> <p>Results</p> <p>Self-assembling autofluorescent protein microarrays were produced by <it>in situ </it>transcription and translation of chimeric proteins containing a C-terminal Green Fluorescent Protein tag. Proteins were immobilized as array elements using an anti-GFP monoclonal antibody. The amounts of correctly-folded chimeric proteins were quantified by measuring the fluorescence intensity from each array element. During cell-free expression, very little or no fluorescence was observed from GFP-tagged multidomain eukaryotic plant proteins when <it>in vitro </it>translation was performed with <it>E. coli </it>S30 extract. Improvement was seen using wheat germ extract, but fluorescence intensities were still low because of poor protein yields. A hybrid <it>in vitro </it>translation system, combining S30 and wheat germ extracts, produced high levels of correctly-folded proteins for most of the constructs that were tested.</p> <p>Conclusion</p> <p>The results are consistent with the hypothesis that the wheat germ extract enhances the protein folding capabilities of the <it>in vitro </it>system by providing eukaryotic ribosomes and chaperones and, at the same time, the <it>E. coli </it>S30 extract, which includes an ATP regeneration system, translates the polypeptides at high rates. This hybrid cell-free expression system allows the facile production of high-yield protein arrays suitable for downstream assays.</p