Trichloro(sulfanyl)ethyl benzamides inhibit AITC (80 μM) induced increase in intracellular calcium and inward currents in CHO cells stably expressing human TRPA1

<p><b>Copyright information:</b></p><p>Taken from "Species-specific pharmacology of Trichloro(sulfanyl)ethyl benzamides as transient receptor potential ankyrin 1 (TRPA1) antagonists"</p><p>http://www.molecularpain.com/content/3/1/39</p><p>Molecular Pain 2007;3():39-39.</p><p>Published online 17 Dec 2007</p><p>PMCID:PMC2222611.</p><p></p> Effect of AMG9090 , AMG5445 , AMG2504 , and AMG7160 on AITC-induced increase in intracellular calcium in CHO cells expressing human TRPA1 measured in an aequorin-readout assay. Open circles represent the response of cells to the compound itself in the absence of agonist. Each point in the graph is an average ± SEM of an experiment conducted in triplicate

Effect of human TRPA1 antagonists on CHO cells stably expressing rat TRPA1

<p><b>Copyright information:</b></p><p>Taken from "Species-specific pharmacology of Trichloro(sulfanyl)ethyl benzamides as transient receptor potential ankyrin 1 (TRPA1) antagonists"</p><p>http://www.molecularpain.com/content/3/1/39</p><p>Molecular Pain 2007;3():39-39.</p><p>Published online 17 Dec 2007</p><p>PMCID:PMC2222611.</p><p></p> Concentration-response curves generated in aequorin-readout assay Note, AMG9090 and AMG5445 acted as partial agonists. Each point in the graph is presented as percent of AITC response and is an average ± SEM of an experiment conducted in triplicate. Representative traces of inward currents induced by AITC, AMG9090 and AMG5445 are shown in , , and respectively. AITC-induced increase in intracellular calcium in CHO cells expressing rat TRPA1 in the absence (100%) or presence of different concentrations of AMG2504 and AMG 7160. Representative current traces induced by AITC in the presence of AMG2504 and AMG 7160 are shown in and , respectively

Trichloro(sulfanyl)ethyl benzamides inhibit cold temperature (4°C) induced Cauptake in CHO cells stably expressing human TRPA1

<p><b>Copyright information:</b></p><p>Taken from "Species-specific pharmacology of Trichloro(sulfanyl)ethyl benzamides as transient receptor potential ankyrin 1 (TRPA1) antagonists"</p><p>http://www.molecularpain.com/content/3/1/39</p><p>Molecular Pain 2007;3():39-39.</p><p>Published online 17 Dec 2007</p><p>PMCID:PMC2222611.</p><p></p> Concentration-response curves were generated utilizing Cauptake assays as described under . Each point in the graph is an average ± SEM of an experiment conducted in duplicate

Characterization of CHO cell lines stably expressing human and rat TRPA1

<p><b>Copyright information:</b></p><p>Taken from "Species-specific pharmacology of Trichloro(sulfanyl)ethyl benzamides as transient receptor potential ankyrin 1 (TRPA1) antagonists"</p><p>http://www.molecularpain.com/content/3/1/39</p><p>Molecular Pain 2007;3():39-39.</p><p>Published online 17 Dec 2007</p><p>PMCID:PMC2222611.</p><p></p> FLASH luminometer was used to measure concentration-dependent AITC-induced increase in intracellular calcium, and concentration-dependent inhibition of AITC (80 μM) activation by ruthenium red in CHO cells expressing human and rat TRPA1. Each point in the graph is an average ± SEM of an experiment conducted in triplicate. Maximum response of AITC (80 μM) was normalized to 100%. Cold activation profiles of CHO cells expressing human and rat TRPA1 were characterized utilizing cold-induced Cauptake assay. Cauptake by CHO cells and CHO cells transfected with human TRPA1 in response to stimulation with temperatures between 3.5 and 25°C. Cold temperature (4°C) activation of un-induced and tetracycline-induced CHO cells transfected with either human or rat TRPA1. Concentration-dependent inhibition of cold (4°C) activation by ruthenium red in CHO cells expressing human and rat TRPA1. Each point in the graph is an average ± SEM of an experiment conducted in duplicate

Trichloro(sulfanyl)ethyl benzamides inhibit AITC (80 μM) induced inward currents in CHO cells stably expressing human TRPA1

<p><b>Copyright information:</b></p><p>Taken from "Species-specific pharmacology of Trichloro(sulfanyl)ethyl benzamides as transient receptor potential ankyrin 1 (TRPA1) antagonists"</p><p>http://www.molecularpain.com/content/3/1/39</p><p>Molecular Pain 2007;3():39-39.</p><p>Published online 17 Dec 2007</p><p>PMCID:PMC2222611.</p><p></p> Representative traces of inward currents evoked by AITC in the absence or presence of AMG9090 , AMG5445 , AMG2504 , and AMG7160 are shown. ICvalue for each compound was determined from their concentration-dependent inhibition of AITC-induced currents using a PatchXpress 7000A workstation