<p><b>Copyright information:</b></p><p>Taken from "Species-specific pharmacology of Trichloro(sulfanyl)ethyl benzamides as transient receptor potential ankyrin 1 (TRPA1) antagonists"</p><p>http://www.molecularpain.com/content/3/1/39</p><p>Molecular Pain 2007;3():39-39.</p><p>Published online 17 Dec 2007</p><p>PMCID:PMC2222611.</p><p></p> FLASH luminometer was used to measure concentration-dependent AITC-induced increase in intracellular calcium, and concentration-dependent inhibition of AITC (80 μM) activation by ruthenium red in CHO cells expressing human and rat TRPA1. Each point in the graph is an average ± SEM of an experiment conducted in triplicate. Maximum response of AITC (80 μM) was normalized to 100%. Cold activation profiles of CHO cells expressing human and rat TRPA1 were characterized utilizing cold-induced Cauptake assay. Cauptake by CHO cells and CHO cells transfected with human TRPA1 in response to stimulation with temperatures between 3.5 and 25°C. Cold temperature (4°C) activation of un-induced and tetracycline-induced CHO cells transfected with either human or rat TRPA1. Concentration-dependent inhibition of cold (4°C) activation by ruthenium red in CHO cells expressing human and rat TRPA1. Each point in the graph is an average ± SEM of an experiment conducted in duplicate
