To Transplant or Not to Transplant? The Successful Treatment of a Lateral Meniscus Transplant

The purpose of this case report was to demonstrate how physical therapists can successfully manage a young patient who received the rare lateral meniscal transplant procedure.https://soar.usa.edu/flsaspring2018/1011/thumbnail.jp

Ease into It. Effects of Mobilization with Movement Utilizing Patients Body Weight

The purpose of this case report is to utilize mobilization with movement (MWM) to restore knee flexion in a patient with delayed quadriceps tendon repair.https://soar.usa.edu/flsaspring2018/1002/thumbnail.jp

Joint Mobilizations and Paraplegia: A Curious Pairing

The purpose of this case report is to explore the effectiveness of joint mobilizations at the knee on gait and pain in a patient with ambulatory paraplegia.https://soar.usa.edu/flsasummer2018/1013/thumbnail.jp

Are You Ready to get High? High Repetition Exercise for Radial Nerve Palsy

Research suggests the need for early surgical nerve exploration for secondary radial nerve palsy based upon a 75% recovery rate with early exploration.5 In using typical conservative management plus high-repetition movement, the patient in this case report was able to avoid surgery for radial nerve exploration, attain complete recovery within 4 months of his initial injury, and fully return to work and daily function. This case report offers the possibility of high-repetition movement being a beneficial treatment intervention. This is a simple, low-cost treatment and can be accomplished by the patient at home as part of a home exercise program. For this patient, full recovery was attained without additional surgery for nerve exploration. Perhaps this case report serves as a catalyst for further research in the area of radial nerve palsy in applying a conservative treatment approach that aids in the regeneration of the radial nerve without having to turn towards surgical interventions.https://soar.usa.edu/flsafall2018/1001/thumbnail.jp

Proprioceptive Neuromuscular Facilitation In A Male Wrestler Post Type II Slap Lesion Surgical Repair

The primary purpose of this case report was to assess the effects of a specific progression of PNF and Rhythmic Stabilization Exercises (RSE) in conjunction with traditional physical therapy for a seventeen-year-old wrestler post Type II SLAP lesion surgical repair. This case report indicated that the utilization of PNF and RSE is a beneficial component to physical therapy treatment of a seventeen-year-old wrestling athlete with a Type II SLAP lesion surgical repair. Designing a treatment plan with emphasis on PNF and RSE is cost-efficient, as it does not require expensive equipment or additional certifications. Also, physical therapists utilizing PNF and RSE could further control the safety of therapeutic interventions by providing manually controlled resistance and perturbations.https://soar.usa.edu/flsafall2018/1012/thumbnail.jp

Upping the Ante!: Physical Therapist Management of Femoral Anteversion

The purpose of this case report is to describe the outcomes following the rehabilitation of an 11-year-old female athlete with femoral anteversion utilizing Pilates based therapy in conjunction with traditional physical therapy.https://soar.usa.edu/flsaspring2017/1019/thumbnail.jp

Magnetic Photon Splitting: the S-Matrix Formulation in the Landau Representation

Calculations of reaction rates for the third-order QED process of photon splitting in strong magnetic fields traditionally have employed either the effective Lagrangian method or variants of Schwinger's proper-time technique. Recently, Mentzel, Berg and Wunner (1994) presented an alternative derivation via an S-matrix formulation in the Landau representation. Advantages of such a formulation include the ability to compute rates near pair resonances above pair threshold. This paper presents new developments of the Landau representation formalism as applied to photon splitting, providing significant advances beyond the work of Mentzel et al. by summing over the spin quantum numbers of the electron propagators, and analytically integrating over the component of momentum of the intermediate states that is parallel to field. The ensuing tractable expressions for the scattering amplitudes are satisfyingly compact, and of an appearance familiar to S-matrix theory applications. Such developments can facilitate numerical computations of splitting considerably both below and above pair threshold. Specializations to two regimes of interest are obtained, namely the limit of highly supercritical fields and the domain where photon energies are far inferior to that for the threshold of single-photon pair creation. In particular, for the first time the low-frequency amplitudes are simply expressed in terms of the Gamma function, its integral and its derivatives. In addition, the equivalence of the asymptotic forms in these two domains to extant results from effective Lagrangian/proper-time formulations is demonstrated.Comment: 19 pages, 3 figures, REVTeX; accepted for publication in Phys. Rev.

IL-17 Receptor Signaling in Oral Epithelial Cells Is Critical for Protection against Oropharyngeal Candidiasis

Signaling through the IL-17 receptor (IL-17R) is required to prevent oropharyngeal candidiasis (OPC) in mice and humans. However, the IL-17-responsive cell type(s) that mediate protection are unknown. Using radiation chimeras we were able to rule out a requirement for IL-17RA in the hematopoietic compartment. We saw remarkable concordance of IL-17-controlled gene expression in C. albicans-infected human oral epithelial cells (OECs) and in tongue tissue from mice with OPC. To interrogate the role of the IL-17R in OECs, we generated mice with conditional deletion of IL-17RA in superficial oral and esophageal epithelial cells (Il17ra(ΔK13)). Following oral Candida infection, Il17ra(ΔK13) mice exhibited fungal loads and weight loss indistinguishable from Il17ra(−/−) mice. Susceptibility in Il17ra(ΔK13) mice correlated with expression of the antimicrobial peptide β-defensin 3 (BD3, Defb3). Consistently, Defb3(−/−) mice were susceptible to OPC. Thus, OECs dominantly control IL-17R-dependent responses to OPC through regulation of BD3expression

Glutamine versus Ammonia Utilization in the NAD Synthetase Family

NAD is a ubiquitous and essential metabolic redox cofactor which also functions as a substrate in certain regulatory pathways. The last step of NAD synthesis is the ATP-dependent amidation of deamido-NAD by NAD synthetase (NADS). Members of the NADS family are present in nearly all species across the three kingdoms of Life. In eukaryotic NADS, the core synthetase domain is fused with a nitrilase-like glutaminase domain supplying ammonia for the reaction. This two-domain NADS arrangement enabling the utilization of glutamine as nitrogen donor is also present in various bacterial lineages. However, many other bacterial members of NADS family do not contain a glutaminase domain, and they can utilize only ammonia (but not glutamine) in vitro. A single-domain NADS is also characteristic for nearly all Archaea, and its dependence on ammonia was demonstrated here for the representative enzyme from Methanocaldococcus jannaschi. However, a question about the actual in vivo nitrogen donor for single-domain members of the NADS family remained open: Is it glutamine hydrolyzed by a committed (but yet unknown) glutaminase subunit, as in most ATP-dependent amidotransferases, or free ammonia as in glutamine synthetase? Here we addressed this dilemma by combining evolutionary analysis of the NADS family with experimental characterization of two representative bacterial systems: a two-subunit NADS from Thermus thermophilus and a single-domain NADS from Salmonella typhimurium providing evidence that ammonia (and not glutamine) is the physiological substrate of a typical single-domain NADS. The latter represents the most likely ancestral form of NADS. The ability to utilize glutamine appears to have evolved via recruitment of a glutaminase subunit followed by domain fusion in an early branch of Bacteria. Further evolution of the NADS family included lineage-specific loss of one of the two alternative forms and horizontal gene transfer events. Lastly, we identified NADS structural elements associated with glutamine-utilizing capabilities

Parps: Rapidly Evolving Weapons in the War against Viral Infection

Post-translational protein modifications such as phosphorylation and ubiquitinylation are common molecular targets of conflict between viruses and their hosts. However, the role of other post-translational modifications, such as ADP-ribosylation, in host-virus interactions is less well characterized. ADP-ribosylation is carried out by proteins encoded by the PARP (also called ARTD) gene family. The majority of the 17 human PARP genes are poorly characterized. However, one PARP protein, PARP13/ZAP, has broad antiviral activity and has evolved under positive (diversifying) selection in primates. Such evolution is typical of domains that are locked in antagonistic 'arms races' with viral factors. To identify additional PARP genes that may be involved in host-virus interactions, we performed evolutionary analyses on all primate PARP genes to search for signatures of rapid evolution. Contrary to expectations that most PARP genes are involved in 'housekeeping' functions, we found that nearly one-third of PARP genes are evolving under strong recurrent positive selection. We identified a >300 amino acid disordered region of PARP4, a component of cytoplasmic vault structures, to be rapidly evolving in several mammalian lineages, suggesting this region serves as an important host-pathogen specificity interface. We also found positive selection of PARP9, 14 and 15, the only three human genes that contain both PARP domains and macrodomains. Macrodomains uniquely recognize, and in some cases can reverse, protein mono-ADP-ribosylation, and we observed strong signatures of recurrent positive selection throughout the macro-PARP macrodomains. Furthermore, PARP14 and PARP15 have undergone repeated rounds of gene birth and loss during vertebrate evolution, consistent with recurrent gene innovation. Together with previous studies that implicated several PARPs in immunity, as well as those that demonstrated a role for virally encoded macrodomains in host immune evasion, our evolutionary analyses suggest that addition, recognition and removal of ADP-ribosylation is a critical, underappreciated currency in host-virus conflicts