Histological changes on testes of mice administered with different concentrations of Cosmos caudatus ethanolic extract

The histological changes of testes of mice were assessed after 28 days treatment with different concentrations of Cosmos caudatus ethanolic extract (50, 125, 500 and 1000 mg/kg). Testicular tissues were fixed in 10% formalin, embedded in paraffin wax and cut for histological study. The qualitative assessment of histological changes of the testes showed different degrees of thickness and density of seminiferous tubules as rated according to the following scales: 1, thin and low density of seminiferous tubules; 2, moderately thick and moderately dense seminiferous tubules; 3, thick and dense seminiferous tubules. Scale 3 was observed in testes of mice treated with 500 and 1000 mg/kg b wt., scale 2 in group treated with 50 mg/ kg b.wt. and scale 1 in group treated with 125 mg/kg b wt. of the extract. The total percentage of condensed seminiferous tubules (seminiferous tubules without lumen) is highest in the testes of mice from 500 mg/kg b wt. (52.5%) and the lowest from 125 mg/kg b wt. (26.3%). Both groups from 500 and 1000 mg/kg b wt. showed significant results (p<0.05) which the thickness of the seminiferous tubules increased as compared with the control group of mice. Hence, the higher concentration of C. caudatus gave better effects on spermatogenic activity of the testes of mice, as revealed by the density and thickness of the seminiferous tubules

THE EFFECTS OF COSMOS CAUDATUS AND PIPER SARMENTOSUM AQUEOUS EXTRACTS ON MALE MICE FERTILITY

Objective: This study was conducted to determine the sperm quality and fertility of male mice supplemented with Cosmos caudatus and Piper sarmentosum aqueous extracts.Methods: The male mice were treated with either 0.2 ml of distilled water, 500 mg/kg bwt of Cosmos caudatus or Piper sarmentosum aqueous extract for the duration of four weeks. The body weight was checked on a weekly basis. Treated male mice then were mated with proven fertile females and the numbers of implantation sites were counted. At the end of the experimental duration, treated males were sacrificed by cervical dislocation and subjected to sperm analysis.Results: Our data revealed that Cosmos caudatus and Piper sarmentosum increased the sperm quality and fertility in male mice. In addition, we found that the body weight was not affected by Cosmos caudatus or Piper sarmentosum aqueous extract.Conclusion: In conclusion, Cosmos caudatus and Piper sarmentosum produced a remarkable increase in male mice reproductive performance.Â

ANTI-PYRETIC ACTION OF Caulerpa lentillifera, Hibiscus rosa-sinensis AND Piper sarmentosum AQUEOUS EXTRACT IN MICE

Objective: The present study was conducted to evaluate the anti-pyretic activity of Caulerpa lentillifera, Hibiscus rosa-sinensis, and Piper sarmentosumaqueous extracts.Methods: The suspension of yeast was injected intraperitoneally (0.1 g/kg of bodyweight [BWT]) to induce fever in experimental animals. Rectaltemperature was recorded every hour with a lubricated digital thermometer and animals with at least 0.7°C elevations in body core temperaturewere included in the experiment. The animals with fever were administered orally with aqueous extracts of C. lentillifera, H. rosa-sinensis, andP. sarmentosum (500 mg/kg of BWT). Another group was given the commercial drug, acetaminophen (10 mg/kg of BWT), to serve as a control.Results: The result of the study showed that C. lentillifera and H. rosa-sinensis aqueous extracts significantly (p&lt;0.05) effective in combating fever.However, P. sarmentosum aqueous extract has no potential as an anti-pyretic agent.Conclusion: H. rosa-sinensis and C. lentillifera aqueous extracts have anti-pyretic potential but not P. sarmentosum aqueous extract.Keywords: Caulerpa lentillifera, Hibiscus rosa-sinensis, Piper sarmentosum, Anti-pyretic, Fever, Pyrexia, Yeast

The effects of curculigo latifolia dry and ethanolic extracts on sperm quality of mice mus musculus / Faridahanim Mohd Jaafar

Several Curculigo species have been reported to be widely used for the treatment of impotence and act as aphrodisiac. However, the effect of Curculigo latifolia Dryand on sperm quality, defined as the measurable parameters of the sperm sample which may determine its fertilising capacity, has not been completely explored. The objective of this study was to determine and compare the effects of the leaf and root extracts of Curculigo latifolia on the sperm quality of mice Mus musculus. Three groups of male mice were used in this study; the control group (Group 1) was forced-fed with saline solution, Group 2 with 500 mg/kg b wt of the root extract of C. latifolia and Group 3 with 500 mg/kg b wt of the leaf extract of the plant. The treatments were conducted for a duration of 14 days. The results of this study gave no significant difference in the weekly body weight and sperm morphology of the mice. Mice fed with the root extract showed a greater increase in sperm motility (75.33±2.60%) compared to Group 3 (74.00±5.00%) and Group 1 (64.33±2.73%). Meanwhile, mice fed with the leaf extract showed a higher sperm count (62.33±4.33 mill/ml) and sperm viability (28.00±4.04%) compared to Group 2 (58.67±4.10 mill/ml and 27.67±5.55%, respectively) and Group 1 (40.33±3.18 mill/ml and 23.33±2.96%, respectively). In conclusion, the leaf and root extracts of Curculigo latifolia appeared to have positive effects on sperm quality parameters which are linked to male fertility

Curative potential of Zingiber zerumbet on paracetamol-induced hepatotoxicity

Zingiber zerumbet is a type of wild ginger known worldwide for its medicinal values. Its constituents found in the rhizome suggest potent antioxidant and anti-inflammatory activities. As such, this study investigated the possible curative effects of Zingiber zerumbet rhizome ethanolic extract (ZZ) on Paracetamol (PCM)-induced hepatotoxicity. The plant was obtained from Sendayan, Negeri Sembilan and had been identified by the Biodiversity Unit of Universiti Putra Malaysia (UPM), Malaysia. Its rhizomes were processed and extracted with 80% ethanol. The chemical profile of the extract was determined using Gas Chromatography-Mass Spectrometry (GC-MS) analysis, while hepatotoxic rats were treated orally with varied extract doses (250, 350, and 450 mg/kg bwt) for a week, and N-acetyl-L-cysteine (NAC) was used as reference drug. At the end of the treatment, sera samples were collected for liver function tests (LFTs). The chemical profile of the extract determined via GC-MS analysis revealed three major compounds that contributed to the bioactivity of the plant. Zerumbone had the highest component concentration (95%) in the extract for GC-MS study. Significant improvements in LFT parameters (alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate aminotransferase (AST), and total protein (TP) levels) were also observed in groups that received 350 and 450 mg/kg bwt extract, wherein the 350 mg/kg bwt dose appeared to be the most effective dose that reduced liver enzyme markers in PCM-induced hepatotoxic rats. Overall, Z. zerumbet exhibited the most optimum hepatocurative potential in enhancing liver functions amidst PCM-induced hepatotoxic rats at 350 mg/kg bwt dose

Effects of Cosmos caudatus on sperm quality of mice, Mus musculus

This study was designed to analyse the effects of Cosmos caudatus on sperm quality of mice, Mus musculus. The daily intake of Cosmos caudatus was evaluated in determining its effects on male fertility. Four different concentrations of the crude ethanolic extracts which were 50 mg/kg, 125 mg/kg, 500 mg/kg and 1000 mg/kg, respectively were administered for 28 consecutive days. Sperm analyses includes sperm count, total sperm motility, sperm viability and sperm morphology were carried out using standard procedures outlined by WHO 2010. G3 group gives the highest mean percentage for change in body weight (9.60 gm), total sperm motility (56%) and normal sperm morphology count (62.8 sperm) as compared to the rest the group treatments. For the sperm count and sperm viability, G4 group showed the highest data; 56.5 million sperm/ml and 59.53%, respectively. The lowest data of the change in body weight and normal sperm morphology count of mice from G1 group which are 6.43 gm and 58.6 sperm, correspondingly. As for the sperm count, total sperm motility and sperm viability; the lowest data shown by G2 group; 39.4 million sperm/ml, 48% and 43.98%, respectively. The sperm morphology of all the treated male mice was normal except a few detected with abnormalities. The findings showed no negative effect of Cosmos caudatus on sperm quality of mice