Drug interference in Trinder reaction

Our recent research of drug interference in Trinder reaction – a widely used chromogenic reaction in the field of clinical biochemistry, was focused on comparison of various drugs and the impact on determination of several biochemical parameters with validated diagnostic kits utilizing Trinder reaction. Our results showed significant alteration of results in therapeutic concentrations of ACC®, Dicynone® and Novalgin® in comparison to the control samples. Dobutamine, Tensamin and ascorbic acid showed significant interference only in samples containing 1 mmol/l of drug.V našem posledním výzkumu lékové interference v Trinderově reakci, široce rozšířené chromogenní reakci v oboru klinické biochemie, jsme se zaměřili na porovnání různých leků a jejich dopad na měření několika biochemických paramerů pomocí validovaných diagnostických souprav využívající Trinderovu reakci. Naše výsledky ukázaly významnou změnu výsledků při terapeutických koncentracích ACC, Dicynonu a Novalginu ve srovnání s kontrolními vzorky. Dobutamin, Tensamin a kyselina askorbová projevovaly významnou interferenci až u vzorků obsahujícíc koncentraci léku 1 mmol/l

Interference Dicynone v Trinderově reakci – porovnání produktů dodavatelů IVD

V této části našeho výzkumu jsme se zaměřili na porovnání interference Trinderovy reakce lékem Dicynone, u reagenčních setů různých výrobců in vitro diagnostiky působící v České republice. Trinderova reakce je v klinické biochemii poměrně rozšířená chromogenní reakce a Dicynone je široce užívané hemostatikum. Na výsledcích ukazujeme jednak to, že žádný z výrobců tuto interferenci zatím spolehlivě nevyřešil, ale také to, že se dá do značné míry omezit, jak lze uvést na příkladu fy Erba Lachema.This section of our Dicynone interference in Trinder reaction research was focused on performance of different in vitro diagnostic providers and their reagent sets from located in Czech Republic. Trinder reaction is a widely used chromogenic reaction and Dicynone is a abundantly used hemostatic medication. The results show that the interference is universal in all reagent kits utilizing Trinder reaction and also that the interference effect could be reduced as is shown in Erba Lachema company results

Different Clinical Utility of Oropharyngeal Bacterial Screening prior to Percutaneous Endoscopic Gastrostomy in Oncological and Neurological Patients

Background. The aim of this study was to monitor oropharyngeal bacterial colonization in patients indicated for percutaneous endoscopic gastronomy (PEG). Methods. Oropharyngeal swabs were obtained from patients prior to PEG placement. A development of peristomal infection was evaluated. The analysis of oropharyngeal and peristomal site pathogens was done. Results. Consecutive 274 patients referred for PEG due to neurological disorder or cancer completed the study. Oropharyngeal colonization with pathogens was observed in 69% (190/274), dominantly in the neurologic subgroup of patients (P < 0.001). Peristomal infection occurred in 30 (10.9%) of patients and in 57% of them the correlation between oropharyngeal and peristomal agents was present. The presence of oropharyngeal pathogens was assessed as an important risk factor for the development of peristomal infection only in oncological patients (OR = 8.33, 95% CI: 1.66–41.76). Despite a high prevalence of pathogens in neurological patients, it did not influence the risk of peristomal infection with the exception for methicillin resistant Staphylococcus aureus (MRSA) carriers (OR 4.5, 95% CI: 1.08–18.76). Conclusion. During oropharyngeal microbial screening prior to the PEG insertion, the detection of pathogens may be a marker of the increased risk of peristomal infection in cancer patients only. In neurological patients the benefit of the screening is limited to the detection of MRSA carriers

Different Clinical Utility of Oropharyngeal Bacterial Screening prior to Percutaneous Endoscopic Gastrostomy in Oncological and Neurological Patients

Background. The aim of this study was to monitor oropharyngeal bacterial colonization in patients indicated for percutaneous endoscopic gastronomy (PEG). Methods. Oropharyngeal swabs were obtained from patients prior to PEG placement. A development of peristomal infection was evaluated. The analysis of oropharyngeal and peristomal site pathogens was done. Results. Consecutive 274 patients referred for PEG due to neurological disorder or cancer completed the study. Oropharyngeal colonization with pathogens was observed in 69% (190/274), dominantly in the neurologic subgroup of patients (P < 0.001). Peristomal infection occurred in 30 (10.9%) of patients and in 57% of them the correlation between oropharyngeal and peristomal agents was present. The presence of oropharyngeal pathogens was assessed as an important risk factor for the development of peristomal infection only in oncological patients (OR = 8.33, 95% CI: 1.66–41.76). Despite a high prevalence of pathogens in neurological patients, it did not influence the risk of peristomal infection with the exception for methicillin resistant Staphylococcus aureus (MRSA) carriers (OR 4.5, 95% CI: 1.08–18.76). Conclusion. During oropharyngeal microbial screening prior to the PEG insertion, the detection of pathogens may be a marker of the increased risk of peristomal infection in cancer patients only. In neurological patients the benefit of the screening is limited to the detection of MRSA carriers

Stereotactic Body Radiotherapy (SBRT) of Pancreatic Cancer&mdash;A Critical Review and Practical Consideration

Pancreatic cancer is the third leading cause of cancer death in the developed world and is predicted to become the second by 2030. A cure may be achieved only with surgical resection of an early diagnosed disease. Surgery for more advanced disease is challenging and can be contraindicated for many reasons. Neoadjuvant therapy may improve the probability of achieving R0 resection. It consists of systemic treatment followed by radiation therapy applied concurrently or sequentially with cytostatics. A novel approach to irradiation, stereotactic body radiotherapy (SBRT), has the potential to improve treatment results. SBRT can deliver higher doses of radiation to the tumor in only a few treatment fractions. It has attracted significant interest for pancreatic cancer patients, as it is completed quickly, requires less time away from full-dose chemotherapy, and is well-tolerated than conventional radiotherapy. In this review, we aim to provide the reader with a basic overview of current evidence for SBRT indications in the treatment of pancreatic tumors. In the second part of the review, we focus on practical information with respect to SBRT treatment plan preparation the performance of such therapy. Finally, we discuss future directions related to the use of magnetic resonance linear accelerators

Clinical Biochemistry

Učebnice Clinical Biochemistry ve svých 39 kapitolách pokrývá všechna důležitá témata moderní klinické biochemie. Od preanalytických vlivů na laboratorní vyšetření přes analytické a klinické vlastnosti metod, kontrolu kvality v klinické laboratoři, automatizaci po jednotlivé indikace a interpretace laboratorních testů v diagnostice a sledování různých onemocnění. Cílem je dovést čtenáře k propojení všech aspektů ovlivňujících laboratorní výsledek a jejich kritickému zhodnocení při péči o pacienta. Text je určen především pro pregraduální studenty všeobecného lékařství, avšak jako referenční příručku ji využijí i začínající lékaři všech oborů. Clinical Biochemistry textbook covers in its 39 chapters all important topics of modern clinical biochemistry. From preanalytical influences to laboratory results through analytical and clinical properties of laboratory methods, quality control in Clinical laboratory, automation to indication and interpretation of laboratory tests in diagnosis and follow up of a plenty of diseases. The goal of this publication is to lead a reader to intersection of all aspects that influence laboratory results and to its critical evaluation in taking care about a patient. The text is designed to pregradual students of general medicine. However, also young medical doctors of all medical specializations can use the textbook as a reference guide

Clinical Biochemistry

Učebnice Clinical Biochemistry ve svých 39 kapitolách pokrývá všechna důležitá témata moderní klinické biochemie. Od preanalytických vlivů na laboratorní vyšetření přes analytické a klinické vlastnosti metod, kontrolu kvality v klinické laboratoři, automatizaci po jednotlivé indikace a interpretace laboratorních testů v diagnostice a sledování různých onemocnění. Cílem je dovést čtenáře k propojení všech aspektů ovlivňujících laboratorní výsledek a jejich kritickému zhodnocení při péči o pacienta. Text je určen především pro pregraduální studenty všeobecného lékařství, avšak jako referenční příručku ji využijí i začínající lékaři všech oborů. Clinical Biochemistry textbook covers in its 39 chapters all important topics of modern clinical biochemistry. From preanalytical influences to laboratory results through analytical and clinical properties of laboratory methods, quality control in Clinical laboratory, automation to indication and interpretation of laboratory tests in diagnosis and follow up of a plenty of diseases. The goal of this publication is to lead a reader to intersection of all aspects that influence laboratory results and to its critical evaluation in taking care about a patient. The text is designed to pregradual students of general medicine. However, also young medical doctors of all medical specializations can use the textbook as a reference guide