A clinical study on Infantile Colic with Kuberaksha

Crying is one of the essential behaviors to communicate the demands of the baby so that it can be fulfilled by the parents. Prolonged crying is a source of anxiety and distress for the parents & challenge for the doctor. Infantile colic is a diagnosis of exclusion for prolonged cry in early infancy. It is described as paroxysmal crying. In the present clinical study total 100 patients were treated with 2 groups viz. treated group with Kuberaksha Vati (n=60) and Control group (n=40). By triturating Beejamajja (seed pulp powder) of Kuberaksha with honey, pills were prepared by an approximate weight of 125mg for treated group and for the purpose of controlled group study, placebo were prepared in similar way as that of Kuberaksha Vati. The disease infantile colic has compared with Udarashoola described in Ayurvedic classics. The history was noted by interviewing the parent/ guardian. All Parents were advised to administer the drug 125mg twice daily for a period of 3 days. Follow up of study was done after 7 days for any reoccurrence of symptoms. Significant result was obtained after treatment and follow up in treated group; whereas the percentage of improvement was very low in control group. This randomized, placebo controlled clinical trial on infantile colic is found effective by administration of specially prepared Kuberaksha Vati. It has a positive short-term effect on infantile colic

Expectation of forward-backward rapidity correlations in p+pp+p collisions at the LHC energies

Forward-backward correlation strength ($b$) as a function of pesudorapidity intervals for experimental data from $p+\bar{p}$ non-singly diffractive collisions are compared to PYTHIA and PHOJET model calculations. The correlations are discussed as a function of rapidity window ($\Delta \eta$) symmetric about the central rapidity as well as rapidity window separated by a gap ($\eta_{gap}$) between forward and backward regions. While the correlations are observed to be independent of $\Delta \eta$, it is found to decrease with increase in $\eta_{gap}$. This reflects the role of short range correlations and justifies the use of $\eta_{gap}$ to obtain the accurate information about the physics of interest, the long range correlations. The experimental $b$ value shows a linear dependence on $\ln \sqrt{s}$ with the maximum value of unity being reached at $\sqrt{s}$ = 16 TeV, beyond the top LHC energy. However calculations from the PYTHIA and PHOJET models indicate a deviation from linear dependence on $\ln \sqrt{s}$ and saturation in the $b$ values being reached beyond $\sqrt{s}$ = 1.8 TeV. Such a saturation in correlation values could have interesting physical interpretations related to clan structures in particle production. Strong forward-backward correlations are associated with cluster production in the collisions. The average number of charged particles to which the clusters fragments, called the cluster size, are found to also increase linearly with $\ln \sqrt{s}$ for both data and the models studied. The rate of increase in cluster size vs. $\ln \sqrt{s}$ from models studied are larger compared to those from the data and higher for PHOJET compared to PYTHIA. Our study indicates that the forward-backward measurements will provide a clear distinguishing observable for the models studied at LHC energies.Comment: 15 pages, 14 Figures, accepted for publication in International Journal of Modern Physics

Differential Modulation of Angiogenesis by Erythropoiesis-Stimulating Agents in a Mouse Model of Ischaemic Retinopathy

BACKGROUND: Erythropoiesis stimulating agents (ESAs) are widely used to treat anaemia but concerns exist about their potential to promote pathological angiogenesis in some clinical scenarios. In the current study we have assessed the angiogenic potential of three ESAs; epoetin delta, darbepoetin alfa and epoetin beta using in vitro and in vivo models. METHODOLOGY/PRINCIPAL FINDINGS: The epoetins induced angiogenesis in human microvascular endothelial cells at high doses, although darbepoetin alfa was pro-angiogenic at low-doses (1-20 IU/ml). ESA-induced angiogenesis was VEGF-mediated. In a mouse model of ischaemia-induced retinopathy, all ESAs induced generation of reticulocytes but only epoetin beta exacerbated pathological (pre-retinal) neovascularisation in comparison to controls (p<0.05). Only epoetin delta induced a significant revascularisation response which enhanced normality of the vasculature (p<0.05). This was associated with mobilisation of haematopoietic stem cells and their localisation to the retinal vasculature. Darbepoetin alfa also increased the number of active microglia in the ischaemic retina relative to other ESAs (p<0.05). Darbepoetin alfa induced retinal TNFalpha and VEGF mRNA expression which were up to 4 fold higher than with epoetin delta (p<0.001). CONCLUSIONS: This study has implications for treatment of patients as there are clear differences in the angiogenic potential of the different ESAs

Development of microwave assisted-UV digestion using diluted reagents for the determination of total nitrogen in cereals by ion chromatography

The objective of this work is to develop a microwave assisted-ultraviolet (MW-UV) digestion in the presence of dilute HCl and H2O2 followed by ion chromatography (IC) measurements for the determination of total nitrogen in cereals. This approach (MW-UV-IC) requires lesser time and does not need environmentally hazardous materials as used in Kjeldhal method. Further, the developed method requires only microliter quantities of dilute HCl and few milliliters of H2O2 for the matrix digestion and simultaneous conversion of nitrogen to its ionic species for the subsequent analysis by IC. At the optimized acid concentrations (200 ​μL of 0.1 ​mol ​L−1 HCl) and microwave power, the nitrogen in the cereals flours is converted to nitrate (NO3−), nitrite (NO2−) and ammonium (NH4+) ions. The nitrogen species were separated using IonPac AS-20 and IonPac CS-17 columns and then quantified using suppressed conductivity detection. The method was applied to estimate the total nitrogen in flours of various cereals like; wheat (Triticum aestivum), rice (Oryza sativa), finger millet (Eleusine coracana), jowar (Sorghum) and pearl millet (Pennisetum glaucum). The results obtained using proposed method, were in good agreement with that of Kjeldhal method. Further, the precision of the values obtained by developed method was on par with the Kjeldhal method for all the tested flours as verified by F-test (n ​= ​5 and 95% confidence limit). Additionally, greenness assessment tools like analytical Eco-scale and green analytical procedure index (GAPI) suggested the proposed MW-UV-IC method, for the determination of total nitrogen in cereal flours, to be excellently green and safe