Whole-Genome Sequence of Drug-Resistant Mycobacterium tuberculosis Strain S7, Isolated from a Patient with Pulmonary Tuberculosis

Over the past decades, drug-resistant Mycobacterium tuberculosis strains have presented a significant challenge, with inadequate diagnosis of tuberculosis (TB) cases. Here, we report the draft whole-genome sequence of drug-resistant M. tuberculosis strain S7, which was isolated from a patient from Tripura, India, who was diagnosed with pulmonary TB

A novel procedure for the synthesis of NH3 incorporated VPO phases

It has been observed that as a function of a time delay introduced between the onset of the reduction of V2O5 with hydroxylamine hydrochloride and the addition of phosphoric acid, in the aqueous route preparation of the catalytically important VOHPO4?0.5H2O phase, a series of NH3 incorporated phases are obtained. Up to delay times of 3 min the crystalline VOHPO4?0.5H2O phase is obtained whereas a weakly crystalline hemihydrate is formed at a delay time of 4 min. Longer delay times result in X-ray amorphous phases until at delay time of 10 min and above a new crystalline phase containing mixed-valent vanadium emerges. All the phases obtained with delay times of 4 min and above have NH3 incorporated into them. The weakly crystalline VOHPO4?0.5H2O phase as well as the X-ray amorphous phases undergo transformation to a crystalline vanadyl pyrophosphate phase on calcination at 723 K in spite of having NH3 incorporated into them. The in-situ generation of NH3 during the preparation procedure is a hitherto unreported aspect of VPO chemistry and provides a convenient method for the preparation of novel NH3 incorporated VPO phases which could have strong potential as catalysts for alkane oxidation as well as for ammoxidation reactions

Long chain alkyl amine templated synthesis of a mesostructured lamellar vanadium phosphate phase

n-Hexadecylamine (HDA) has been used as a template to synthesize a mesolamellar vanadium phosphate (VPO) phase. FTIR studies indicate covalent interaction of the amine head with the vanadyl groups of the VPO matrix, in contrast to the other reported syntheses of mesostructured VPO phases which involve ionic interaction between a cationic surfactant and the inorganic matrix. XRD, TEM and solid state 13C MAS NMR studies show that the incorporated HDA induces the formation of a mesolamellar phase with a basal spacing of 44.5 A° . On ageing however, a restructuring of the incorporated HDA is observed resulting in a curvature of the inorganic layers and a decrease of the basal spacing to 38.04

Metal ion incorporated VPO phases as novel catalysts

Vanadium phosphates function as catalysts for the selective oxidation of hydrocarbons with their activity being strongly dependent on their structure. Consequently, attempts have been made to synthesise novel phases in the VPO system through metal ion incorporation and evaluate the catalytic activity of these phases. It is shown that the incorporation of palladium or platinum leads to novel phases which vary according to the method of syntheses. The incorporated metal ions are catalytically active both for oxidation and reduction reactions with the activity varying according to the mode of incorporation. These phases constitute a novel class of compounds in which oxidation centres are substituted or dispersed within a VPO oxidation catalyst

Novel platinum incorporated vanadium phosphates and their catalytic activity

Platinum has been incorporated into the layered vanadyl hydrogen phosphate VOHPO4·0.5H2O in different ways depending upon the medium of its preparation. The phase obtained by the introduction of platinum during the synthesis of VOHPO4·0.5H2O in aqueous medium is a new crystalline phase with mixed-valent vanadium containing Pt2+ species. This phase forms a novel hydrogen insertion compound involving hydrogen spillover from the incorporated platinum onto the VPO matrix. On the other hand, the incorporation of platinum during the synthesis of the hemihydrate in organic medium gives rise to a phase containing both metallic platinum and an amorphous Pt2+ containing VPO phase. Both these platinum incorporated phases have been found to be active catalysts for reduction and oxidation reactions such as the hydrogenation of nitrobenzene and the oxidation of tetrahydrofuran with the activity varying quite markedly with the mode of incorporation. © 2002 Elsevier Science B.V. All rights reserved

Mesolamellar VPO phases obtained by incorporating long chain alkyl amine surfactants into the layeredvanadium phosphate dihydrate phase

A series of mesolamellar VPO-amine phases have been obtained by intercalation of alkyl amines of differing alkyl chain lengths into the catalytically important VOPO4 Æ2H2O phase. It is demonstrated that there is considerable variation in the conformation of the incorporated alkyl amines, which progressively assume a more disordered gauche conformation in their internal methylene groups with decreasing chain length. This finding contradicts the assumption made by previous workers that the alkyl amines incorporated into VPO-amine nanocomposites have fully stretched all trans conformations. From the present study, the observation that the basal spacing in long chain amine intercalated mesostructured VPO phases is smaller than that expected from the fully stretched amine molecule can be attributed to the shortening of the incorporated amine molecule due to the gauche conformation of the internal methylene groups. This is in contrast to the postulation of tilted configurations of fully stretched all trans amine molecules, made by earlier workers. It is also observed that the alkyl amine to vanadium ratio increases with increasing alkyl tail length of the incorporated amine, which is consistent with our observation of more ordering in the longer chain amines leading to better packing. Keywords: Vanadium (V) orthophosphate; Alkyl amine surfactant; Intercalation; Mesostructured VPO phases; Conformational heterogeneit

Diagnostic evaluation of pancytopenia - a prospective institutional study in North-East India

Introduction: Pancytopenia, the simultaneous reduction of all the three formed elements of blood, is diverse in its etiology. Full clinico-hematopathological work up is crucial in each case. This study was undertaken with the objectives of identifying the causes of pancytopenia and its phenotypic characteristics in relation to age, sex, ethnicity and socio- economic status. Methods: Ninety two cases of pancytopenia studied here were diagnosed by complete hemogram study in automated hematology analyzer. Peripheral blood smear studies were carried out. All cases were subjected to bone marrow aspiration/ imprint smear cytology and trephine biopsy study. Hypoplastic anemia cases were investigated for paroxysmal nocturnal hemoglobinuria (PNH) with flow cytometric analysis of red blood cells treated with CD55 and CD59 antibodies. Results: Fifty eight percent of cases were males and mean age was 52 years. Children constituted 16% of patients. Thirty-seven percent of cases belonged to tribal community. Around 70% belonged to low socio-economic status. Causes of pancytopenia detected were primary hematological malignancies (Total 39.2 % with acute leukaemia 35.9%, lymphoma 2.2% and multiple myeloma 1.1%), hypoplastic anaemia (28.3%), myelodysplastic syndrome (MDS) (21.7%), megaloblastic Anemia (6.5%) and hypersplenism (4.3%). No PNH clone was detected amongst hypoplastic anemia cases. Conclusion: It is concluded that pancytopenia often is the manifestation of serious disease-processes and the commonest cause, in our population of this North-Eastern state of Tripura, being acute leukemia. Bone marrow cytology and biopsy study must be performed in all cases of pancytopenia to find out the cause in order to institute an early treatment