188 research outputs found
Complete Genome Sequence and Methylome Analysis of Micrococcus luteus SA211, a Halophilic, Lithium-Tolerant Actinobacterium from Argentina
Micrococcus luteus has been found in a wide range of habitats. We report the complete genome sequence and methylome analysis of strain SA211 isolated from a hypersaline, lithium-rich, high-altitude salt flat in Argentina with single-molecule real-time sequencing.Fil: MartĂnez, Fabiana Lilian. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Salta. Instituto de Investigaciones para la Industria QuĂmica. Universidad Nacional de Salta. Facultad de IngenierĂa. Instituto de Investigaciones para la Industria QuĂmica; Argentina. University of Maryland; Estados UnidosFil: Anton, B. P.. New England Biolabs; Estados UnidosFil: DasSarma, P.. University of Maryland; Estados UnidosFil: Rajal, VerĂłnica Beatriz. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Salta. Instituto de Investigaciones para la Industria QuĂmica. Universidad Nacional de Salta. Facultad de IngenierĂa. Instituto de Investigaciones para la Industria QuĂmica; ArgentinaFil: Irazusta, VerĂłnica Patricia. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Salta. Instituto de Investigaciones para la Industria QuĂmica. Universidad Nacional de Salta. Facultad de IngenierĂa. Instituto de Investigaciones para la Industria QuĂmica; ArgentinaFil: Roberts, Roy. New England Biolabs; Estados UnidosFil: DasSarma, S.. University of Maryland; Estados Unido
Spin-isospin textured excitations in a double layer at filling factor
We study the charged excitations of a double layer at filling factor 2 in the
ferromagnetic regime. In a wide range of Zeeman and tunneling splittings we
find that the low energy charged excitations are spin-isospin textures with the
charge mostly located in one of the layers. As tunneling increases, the parent
spin texture in one layer becomes larger and it induces, in the other layer, a
shadow spin texture antiferromagnetically coupled to the parent texture. These
new quasiparticles should be observable by measuring the strong dependence of
its spin on tunneling and Zeeman couplings.Comment: 4 pages, 4 figure
Microbial Fuel Cells Applied to the Metabolically-Based Detection of Extraterrestrial Life
Since the 1970's, when the Viking spacecrafts carried out experiments aimed
to the detection of microbial metabolism on the surface of Mars, the search for
nonspecific methods to detect life in situ has been one of the goals of
astrobiology. It is usually required that the methodology can detect life
independently from its composition or form, and that the chosen biological
signature points to a feature common to all living systems, as the presence of
metabolism. In this paper we evaluate the use of Microbial Fuel Cells (MFCs)
for the detection of microbial life in situ. MFCs are electrochemical devices
originally developed as power electrical sources, and can be described as fuel
cells in which the anode is submerged in a medium that contains microorganisms.
These microorganisms, as part of their metabolic process, oxidize organic
material releasing electrons that contribute to the electric current, which is
therefore proportional to metabolic and other redox processes. We show that
power and current density values measured in MFCs using microorganism cultures
or soil samples in the anode are much larger than those obtained using a medium
free of microorganisms or sterilized soil samples, respectively. In particular,
we found that this is true for extremophiles, usually proposed to live in
extraterrestrial environments. Therefore, our results show that MFCs have the
potential to be used to detect microbial life in situ.Comment: To be published in Astrobiolog
Conductivity in quasi two-dimensional systems
The conductivity in quasi two-dimensional systems is calculated using the
quantum kinetic equation. Linearizing the Lenard-Balescu collision integral
with the extension to include external field dependences allows one to
calculate the conductivity with diagrams beyond the GW approximation including
maximally crossed lines. Consequently the weak localization correction as an
interference effect appears here from the field dependence of the collision
integral (the latter dependence sometimes called intra-collisional field
effect). It is shown that this weak localization correction has the same origin
as the Debye-Onsager relaxation effect in plasma physics. The approximation is
applied to a system of quasi two-dimensional electrons in hetero-junctions
which interact with charged and neutral impurities and the low temperature
correction to the conductivity is calculated analytically. It turns out that
the dynamical screening due to charged impurities leads to a linear temperature
dependence, while the scattering from neutral impurities leads to the usual
Fermi-liquid behavior. By considering an appropriate mass action law to
determine the ratio of charged to neutral impurities we can describe the
experimental metal-insulator transition at low temperatures as a Mott-Hubbard
transition.Comment: 7 pages 7 pages appendix 11 figure
Temperature dependent resistivity of spin-split subbands in GaAs 2D hole system
We calculate the temperature dependent resistivity in spin-split subbands
induced by the inversion asymmetry of the confining potential in GaAs 2D hole
systems. By considering both temperature dependent multisubband screening of
impurity disorder and hole-hole scattering we find that the strength of the
metallic behavior depends on the symmetry of the confining potential (i.e.,
spin-splitting) over a large range of hole density. At low density above the
metal-insulator transition we find that effective disorder reduces the
enhancement of the metallic behavior induced by spin-splitting. Our theory is
in good qualitative agreement with existing experiments
Genetic and transcriptomic analysis of transcription factor genes in the model halophilic Archaeon: coordinate action of TbpD and TfbA
<p>Abstract</p> <p>Background</p> <p>Archaea are prokaryotic organisms with simplified versions of eukaryotic transcription systems. Genes coding for the general transcription factors TBP and TFB are present in multiple copies in several Archaea, including <it>Halobacterium </it>sp. NRC-1. Multiple TBP and TFBs have been proposed to participate in transcription of genes via recognition and recruitment of RNA polymerase to different classes of promoters.</p> <p>Results</p> <p>We attempted to knock out all six TBP and seven TFB genes in <it>Halobacterium </it>sp. NRC-1 using the <it>ura</it>3-based gene deletion system. Knockouts were obtained for six out of thirteen genes, <it>tbp</it>CDF and <it>tfb</it>ACG, indicating that they are not essential for cell viability under standard conditions. Screening of a population of 1,000 candidate mutants showed that genes which did not yield mutants contained less that 0.1% knockouts, strongly suggesting that they are essential. The transcriptomes of two mutants, Δ<it>tbp</it>D and Δ<it>tfb</it>A, were compared to the parental strain and showed coordinate down regulation of many genes. Over 500 out of 2,677 total genes were regulated in the Δ<it>tbp</it>D and Δ<it>tfb</it>A mutants with 363 regulated in both, indicating that over 10% of genes in both strains require the action of both TbpD and TfbA for normal transcription. Culturing studies on the Δ<it>tbp</it>D and Δ<it>tfb</it>A mutant strains showed them to grow more slowly than the wild-type at an elevated temperature, 49°C, and they showed reduced viability at 56°C, suggesting TbpD and TfbA are involved in the heat shock response. Alignment of TBP and TFB protein sequences suggested the expansion of the TBP gene family, especially in <it>Halobacterium </it>sp. NRC-1, and TFB gene family in representatives of five different genera of haloarchaea in which genome sequences are available.</p> <p>Conclusion</p> <p>Six of thirteen TBP and TFB genes of <it>Halobacterium </it>sp. NRC-1 are non-essential under standard growth conditions. TbpD and TfbA coordinate the expression of over 10% of the genes in the NRC-1 genome. The Δ<it>tbp</it>D and Δ<it>tfb</it>A mutant strains are temperature sensitive, possibly as a result of down regulation of heat shock genes. Sequence alignments suggest the existence of several families of TBP and TFB transcription factors in <it>Halobacterium </it>which may function in transcription of different classes of genes.</p
Low-pass sequencing for microbial comparative genomics
BACKGROUND: We studied four extremely halophilic archaea by low-pass shotgun sequencing: (1) the metabolically versatile Haloarcula marismortui; (2) the non-pigmented Natrialba asiatica; (3) the psychrophile Halorubrum lacusprofundi and (4) the Dead Sea isolate Halobaculum gomorrense. Approximately one thousand single pass genomic sequences per genome were obtained. The data were analyzed by comparative genomic analyses using the completed Halobacterium sp. NRC-1 genome as a reference. Low-pass shotgun sequencing is a simple, inexpensive, and rapid approach that can readily be performed on any cultured microbe. RESULTS: As expected, the four archaeal halophiles analyzed exhibit both bacterial and eukaryotic characteristics as well as uniquely archaeal traits. All five halophiles exhibit greater than sixty percent GC content and low isoelectric points (pI) for their predicted proteins. Multiple insertion sequence (IS) elements, often involved in genome rearrangements, were identified in H. lacusprofundi and H. marismortui. The core biological functions that govern cellular and genetic mechanisms of H. sp. NRC-1 appear to be conserved in these four other halophiles. Multiple TATA box binding protein (TBP) and transcription factor IIB (TFB) homologs were identified from most of the four shotgunned halophiles. The reconstructed molecular tree of all five halophiles shows a large divergence between these species, but with the closest relationship being between H. sp. NRC-1 and H. lacusprofundi. CONCLUSION: Despite the diverse habitats of these species, all five halophiles share (1) high GC content and (2) low protein isoelectric points, which are characteristics associated with environmental exposure to UV radiation and hypersalinity, respectively. Identification of multiple IS elements in the genome of H. lacusprofundi and H. marismortui suggest that genome structure and dynamic genome reorganization might be similar to that previously observed in the IS-element rich genome of H. sp. NRC-1. Identification of multiple TBP and TFB homologs in these four halophiles are consistent with the hypothesis that different types of complex transcriptional regulation may occur through multiple TBP-TFB combinations in response to rapidly changing environmental conditions. Low-pass shotgun sequence analyses of genomes permit extensive and diverse analyses, and should be generally useful for comparative microbial genomics
Imaging Coulomb Islands in a Quantum Hall Interferometer
In the Quantum Hall regime, near integer filling factors, electrons should
only be transmitted through spatially-separated edge states. However, in
mesoscopic systems, electronic transmission turns out to be more complex,
giving rise to a large spectrum of magnetoresistance oscillations. To explain
these observations, recent models put forward that, as edge states come close
to each other, electrons can hop between counterpropagating edge channels, or
tunnel through Coulomb islands. Here, we use scanning gate microscopy to
demonstrate the presence of quantum Hall Coulomb islands, and reveal the
spatial structure of transport inside a quantum Hall interferometer. Electron
islands locations are found by modulating the tunneling between edge states and
confined electron orbits. Tuning the magnetic field, we unveil a continuous
evolution of active electron islands. This allows to decrypt the complexity of
high magnetic field magnetoresistance oscillations, and opens the way to
further local scale manipulations of quantum Hall localized states
Complex Effects of Cytochrome P450 Monooxygenase on Purple Membrane and Bacterioruberin Production in an Extremely Halophilic Archaeon: Genetic, Phenotypic, and Transcriptomic Analyses
Halophilic archaea are known to produce a diverse array of pigments for phototrophy and photoprotection. The aim of this paper was to determine the role of a Halobacterium gene encoding the predicted cytochrome P450 monooxygenase (CYP174A1) in pigment synthesis through a combined genetic, phenotypic, and transcriptomic approach. We report on the observed phenotype changes [increased bacterioruberin levels and the loss of purple membrane (PM)] between the Halobacterium salinarum R1 and its CYP174A1-deletion mutant. In addition, we report on the whole-genome DNA microarray analysis, which supports the phenotype of PM loss. This work expands our understanding of the bop-gene regulon, and its relation to carotenoid biosynthesis, and sheds light on our broader understanding of the role (s) of CYP174A1 in archaeal pigment synthesis. To date, this is the first study in which the physiological role of any cytochrome P450 monooxygenase (CYP450) in extremely halophilic archaea has been reported
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