Multi-Nozzle Biopolymer Deposition for Freeform Fabrication of Tissue Constructs

Advanced freeform fabrication techniques have been recently used for the construction of tissue scaffolds because of the process repeatability and capability of high accuracy in fabrication resolution at the macro and micro scales. Among many applicable tissue scaffolding materials, polymeric materials have unique properties in terms of the biocompatibility and degradation, and have thus been widely utilized in tissue engineering applications. Hydrogels, such as alginate, has been one of the most important polymer scaffolding materials because of its biocompatibility and internal structure similarity to that of the extracellular matrix of many tissues, and its relatively moderate processing. Three-dimensional deposition has been an entreating freeform fabrication method of biopolymer and particularly hydrogel scaffolds because of its readiness to deposit fluids at ambient temperatures. This paper presents a recent development of biopolymer deposition based freeform fabrication for 3-diemnsinal tissue scaffolds. The system configuration of multi-nozzles used in the deposition of sodium alginate solutions and Poly-?- Caprolactone (PCL) are described. Studies on polymer deposition feasibility and structural formability are conducted, and the preliminary results are presented.Mechanical Engineerin

Hidden breakpoints in genome alignments

During the course of evolution, an organism's genome can undergo changes that affect the large-scale structure of the genome. These changes include gene gain, loss, duplication, chromosome fusion, fission, and rearrangement. When gene gain and loss occurs in addition to other types of rearrangement, breakpoints of rearrangement can exist that are only detectable by comparison of three or more genomes. An arbitrarily large number of these "hidden" breakpoints can exist among genomes that exhibit no rearrangements in pairwise comparisons. We present an extension of the multichromosomal breakpoint median problem to genomes that have undergone gene gain and loss. We then demonstrate that the median distance among three genomes can be used to calculate a lower bound on the number of hidden breakpoints present. We provide an implementation of this calculation including the median distance, along with some practical improvements on the time complexity of the underlying algorithm. We apply our approach to measure the abundance of hidden breakpoints in simulated data sets under a wide range of evolutionary scenarios. We demonstrate that in simulations the hidden breakpoint counts depend strongly on relative rates of inversion and gene gain/loss. Finally we apply current multiple genome aligners to the simulated genomes, and show that all aligners introduce a high degree of error in hidden breakpoint counts, and that this error grows with evolutionary distance in the simulation. Our results suggest that hidden breakpoint error may be pervasive in genome alignments.Comment: 13 pages, 4 figure

High-dimensional quantum dynamics of adsorption and desorption of H2_2 at Cu(111)

We performed high-dimensional quantum dynamical calculations of the dissociative adsorption and associative desorption of hydrogen on Cu(111). The potential energy surface (PES) is obtained from density functional theory calculations. Two regimes of dynamics are found, at low energies sticking is determined by the minimum energy barrier, at high energies by the distribution of barrier heights. Experimental results are well-reproduced qualitatively, but some quantitative discrepancies are identified as well.Comment: 4 two column pages, revtex, 4 figures, to appear in Phys. Rev. Let

HI and OH absorption in the lensing galaxy of MG J0414+0534

We report the detection of \HI 21-cm absorption in the $z=0.96$ early-type lensing galaxy towards MG J0414+0534 with the Green Bank Telescope. The absorption, with total $N_{\rm HI}=1.6 \times 10^{18} (T_{\rm s}/f) {\rm cm}^{-2}$, is resolved into two strong components, probably due to the two strongest lens components, which are separated by 0.4\arcsec. Unlike the other three lenses which have been detected in \HI, J0414+0534 does not exhibit strong OH absorption, giving a OH/\HI column density ratio of N_{\rm OH}/N_{\rm HI}\lapp10^{-6} (for $T_{\rm s}=100$ K, $T_{\rm x}=10$ K and $f_{\rm HI}=f_{\rm OH}=1$). This underabundance of molecular gas may indicate that the extreme optical--near-IR colour ($V-K=10.26$) along the line-of-sight is not due to the lens. We therefore suggest that despite the strong upper limits on molecular absorption at the quasar redshift, as traced by millimetre lines, the extinction occurs primarily in the quasar host galaxy.Comment: Accepted by MNRAS Letters, 5 (and a bit) pages, 5 figure

Precision Extruding Deposition and Characterization of Cellular Poly-e-Caprolactone Tissue Scaffolds

Successes in scaffold guided tissue engineering require scaffolds to have specific macroscopic geometries and internal architectures in order to provide the needed biological and biophysical functions. Freeform fabrication provides an effective process tool to manufacture many advanced scaffolds with designed properties. This paper reports our recent study on using a novel Precision Extruding Deposition (PED) process technique to directly fabricate cellular Poly-ε-Caprolactone (PCL) scaffolds. Scaffolds with a controlled pore size of 250 µm and designed structural orientations were fabricated. The scaffold morphology, internal micro-architecture and mechanical properties were evaluated using SEM, Micro-Computed Tomography (µ-CT) and the mechanical testing. Preliminary biological study was also conducted to investigate the cell responses to the as-fabricated tissue scaffolds. The results and the characterizations demonstrate the viability of the PED process to the scaffold fabrication as well as a good mechanical property, structural integrity, controlled pore size, pore interconnectivity, and the anticipated biological compatibility of the as-fabricated PCL scaffolds.The authors acknowledge the NSF-0219176 project funding support to graduate students Andrew Darling and Saif Khalil, and the ONR research funding support to graduate student Lauren Shor.Mechanical Engineerin

Thickness-dependent thermal properties of amorphous insulating thin films measured by photoreflectance microscopy

In this work, we report on the measurement of the thermal conductivity of thin insulating films of SiO2 obtained by thermal oxidation, and Al2O3 grown by atomic layer deposition (ALD), both on Si wafers. We used photoreflectance microscopy to determine the thermal properties of the films as a function of thickness in the 2 nm to 1000 nm range. The effective thermal conductivity of the Al2O3 layer is shown to decrease with thickness down to 70% for the thinnest layers. The data were analyzed upon considering that the change in the effective thermal conductivity corresponds to an intrinsic thermal conductivity associated to an additional interfacial thermal resistance. The intrinsic conductivity and interfacial thermal resistance of SiO2 were found to be equal to 0.95 W/m·K and 5.1 × 10− 9 m2K/W respectively; those of Al2O3 were found to be 1.56 W/m·K and 4.3 × 10− 9 m2K/W

Ab initio Quantum and ab initio Molecular Dynamics of the Dissociative Adsorption of Hydrogen on Pd(100)

The dissociative adsorption of hydrogen on Pd(100) has been studied by ab initio quantum dynamics and ab initio molecular dynamics calculations. Treating all hydrogen degrees of freedom as dynamical coordinates implies a high dimensionality and requires statistical averages over thousands of trajectories. An efficient and accurate treatment of such extensive statistics is achieved in two steps: In a first step we evaluate the ab initio potential energy surface (PES) and determine an analytical representation. Then, in an independent second step dynamical calculations are performed on the analytical representation of the PES. Thus the dissociation dynamics is investigated without any crucial assumption except for the Born-Oppenheimer approximation which is anyhow employed when density-functional theory calculations are performed. The ab initio molecular dynamics is compared to detailed quantum dynamical calculations on exactly the same ab initio PES. The occurence of quantum oscillations in the sticking probability as a function of kinetic energy is addressed. They turn out to be very sensitive to the symmetry of the initial conditions. At low kinetic energies sticking is dominated by the steering effect which is illustrated using classical trajectories. The steering effects depends on the kinetic energy, but not on the mass of the molecules. Zero-point effects lead to strong differences between quantum and classical calculations of the sticking probability. The dependence of the sticking probability on the angle of incidence is analysed; it is found to be in good agreement with experimental data. The results show that the determination of the potential energy surface combined with high-dimensional dynamical calculations, in which all relevant degrees of freedon are taken into account, leads to a detailed understanding of the dissociation dynamics of hydrogen at a transition metal surface.Comment: 15 pages, 9 figures, subm. to Phys. Rev.