Testing — and Mostly Rejecting — the Folk Wisdom of the Effective Appellate Brief

There is a great deal of folk wisdom regarding how to draft an effective appellate brief. Judges and lawyers offer advice that briefs should be short, should present relatively few issues, should always be followed by a reply brief, and so forth. There is little doubt that aspiring appellate advocates, law professors who teach writing, appellate court clerks, and appellate court judges look to this folk wisdom to learn how to write effective appellate briefs, teach the skill, and evaluate, by proxies, which briefs are likely to be the best. But this folk wisdom has never been empirically tested. We do so in this article. We examined all cases in the Eighth Circuit Court of Appeals that resulted in an opinion in 2016, coding for many of the variables that the folk wisdom discusses. We also coded for the type of holding and type of opinion that the Eighth Circuit published as our dependent variables. The result was that much of the folk wisdom, in our dataset at least, was not statistically supported. We did, however, find some statistically significant correlations between our independent and dependent variables. These results will help to inform the attorneys, professors, clerks, and courts that work with appellate briefs every day. It will help these people draft, teach, and evaluate appellate briefs. It will also further the inquiry into effective appellate writing by calling into question the accepted folk wisdom, and thus opening the doors for other avenues of research

Chamber investigations of atmospheric mercury oxidation chemistry

Mercury is a potent neurotoxin even at low concentrations. The unoxidised metal has a high vapour pressure and can circulate through the atmosphere, but when oxidised can deposit and be accumulated through the food chain. This work aims to investigate the oxidation processes of atmospheric Hg0(g). The first part describes efforts to make a portable Hg sensor based on Cavity Enhanced Absorption Spectroscopy (CEAS). The detection limit achieved was 66 ngm−3 for a 10 second averaging time. The second part of this work describes experiments carried out in a temperature controlled atmospheric simulation chamber in the Desert Research Institute, Reno, Nevada, USA. The chamber was built around an existing Hg CRDS system that could measure Hg concentrations in the chamber of<100 ngm−3 at 1 Hz enabling reactions to be followed. The main oxidant studied was bromine, which was quantified with a LED based CEAS system across the chamber. Hg oxidation in the chamber was found to be mostly too slow for current models to explain. A seven reaction model was developed and tested to find which parameters were capable of explaining the deviation. The model was overdetermined and no unique solution could be found. The most likely possibility was that the first oxidation step Hg + Br →HgBr was slower than the preferred literature value by a factor of two. However, if the more uncertain data at low [Br2] was included then the only parameter that could explain the experiments was a fast, temperature independent dissociation of HgBr some hundreds of times faster than predicted thermolysis or photolysis rates. Overall this work concluded that to quantitatively understand the reaction of Hg with Br2, the intermediates HgBr and Br must be measured. This conclusion will help to guide the planning of future studies of atmospheric Hg chemistry

Cavity-enhanced absorption using an atomic line source: application to deep-UV measurements

Optical cavities are commonly used to increase the sensitivity of absorption measurements, but have not been extensively used below 300 nm, mainly owing to the limited light sources at these wavelengths. While some progress has been made using cavity ring-down spectroscopy, these systems rely on complex and expensive lasers. Here we investigate an approach combining Cavity-Enhanced Absorption Spectroscopy (CEAS) with an inexpensive low vapour pressure mercury lamp for sensitive absorption measurements at 253.7 nm. We demonstrate that the CEAS absorption in our system is 50 times greater than the absorption found in a single-pass configuration; using this approach, we obtained limits of detection of 8.1 pptv (66 ng m(-3)) for gaseous elemental mercury and 8.4 ppbv for ozone. We evaluate the performance of the system and discuss potential improvements and applications of this approach

COVID-19: In the Absence of Vaccination – ‘Mask-the-Nation’

“In the absence of a vaccine, or effective antiviral, one of our only remaining strategies for controlling COVID-19 is to physically block the spread of SARS-CoV-2 in the community

Survival outcome and EMT suppression mediated by a lectin domain interaction of Endo180 and CD147

Epithelial cell-cell contacts maintain normal glandular tissue homeostasis, and their breakage can trigger epithelial-to-mesenchymal transition (EMT), a fundamental step in the development of metastatic cancer. Despite the ability of C-type lectin domains (CTLD) to modulate cell-cell adhesion, it is not known if they modulate epithelial adhesion in EMT and tumor progression. Here, the multi-CTLD mannose receptor, Endo180 (MRC2/uPARAP), was shown using the Kaplan-Meier analysis to be predictive of survival outcome in men with early prostate cancer. A proteomic screen of novel interaction partners with the fourth CTLD (CTLD4) in Endo180 revealed that its complex with CD147 is indispensable for the stability of three-dimensional acini formed by nontransformed prostate epithelial cells (PEC). Mechanistic study using knockdown of Endo180 or CD147, and treatment with an Endo180 mAb targeting CTLD4 (clone 39.10), or a dominant-negative GST-CTLD4 chimeric protein, induced scattering of PECs associated with internalization of Endo180 into endosomes, loss of E-cadherin (CDH1/ECAD), and unzipping of cell-cell junctions. These findings are the first to demonstrate that a CTLD acts as a suppressor and regulatory switch for EMT; thus, positing that stabilization of Endo180-CD147 complex is a viable therapeutic strategy to improve rates of prostate cancer survival

Arctic Ice Export Events and Their Potential Impact on Global Climate During the Late Pleistocene

Ice sheets in the North American Arctic and, to a lesser extent, those in northern Eurasia calved large quantities of icebergs that drifted through Fram Strait into the Greenland Sea several times during the late Pleistocene. These icebergs deposited Fe oxide grains (45-250 mum) and coarse lithic clasts \u3e250 mum matched to specific circum-Arctic sources. Four massive Arctic iceberg export events are identified from the Laurentide and the Innuitian ice sheets, between 14 and 34 ka (calendar years) in a sediment core from Fram Strait. These relatively short duration (\u3c1-4 kyr) events contain 3-5 times the background levels of Fe oxide grains. They began suddenly, as indicated by a steep rise in the number of grains matched to an ice sheet source, suggesting rapid purges of ice through Fram Strait, due perhaps to collapse of ice sheets. The larger events from the northwestern Laurentide ice sheet are preceded by events from the Innuitian ice sheet. Despite the chronological uncertainties, the Arctic export events appear to occur prior to Heinrich events

AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival

Biomechanical strain imposed by age-related thickening of the basal lamina and augmented tissue stiffness in the prostate gland coincides with increased cancer risk. Here we hypothesized that the structural alterations in the basal lamina associated with age can induce mechanotransduction pathways in prostate epithelial cells (PECs) to promote invasiveness and cancer progression. To demonstrate this, we developed a 3D model of PEC acini in which thickening and stiffening of basal lamina matrix was induced by advanced glycation end-product (AGE)-dependent non-enzymatic crosslinking of its major components, collagen IV and laminin. We used this model to demonstrate that antibody targeted blockade of CTLD2, the second of eight C-type lectin-like domains in Endo180 (CD280, CLEC13E, KIAA0709, MRC2, TEM9, uPARAP) that can recognize glycosylated collagens, reversed actinomyosin-based contractility [myosin-light chain-2 (MLC2) phosphorylation], loss of cell polarity, loss of cell–cell junctions, luminal infiltration and basal invasion induced by AGE-modified basal lamina matrix in PEC acini. Our in vitro results were concordant with luminal occlusion of acini in the prostate glands of adult Endo180ΔEx2–6/ΔEx2–6 mice, with constitutively exposed CTLD2 and decreased survival of men with early (non-invasive) prostate cancer with high epithelial Endo180 expression and levels of AGE. These findings indicate that AGE-dependent modification of the basal lamina induces invasive behaviour in non-transformed PECs via a molecular mechanism linked to cancer progression. This study provides a rationale for targeting CTLD2 in Endo180 in prostate cancer and other pathologies in which increased basal lamina thickness and tissue stiffness are driving factors

Coastal iodine emissions: part 2. Chamber experiments of particle formation from Laminaria digitata-derived and laboratory-generated I2

Laboratory studies into particle formation from Laminaria digitata macroalgae were undertaken to elucidate aerosol formation for a range of I2 (0.3−76 ppbv) and O3(<3−96 ppbv) mixing ratios and light levels (EPAR = 15, 100,and 235 μmol photons m−2 s−1). No clear pattern was observed for I2 or aerosol parameters as a function of light levels. Aerosol mass fluxes and particle number concentrations,were, however, correlated with I2 mixing ratios for low O3mixing ratios of <3 ppbv (R2 = 0.7 and 0.83, respectively for low light levels, and R2 = 0.95 and 0.98, respectively for medium lightlevels). Additional experiments into particle production as a function of laboratory-generated I2, over a mixing ratio range of 1−8ppbv, were conducted under moderate O3 mixing ratios (∼24 ppbv) where a clear, 100-fold or greater, increase in the aeroso lnumber concentrations and mass fluxes was observed compared to the low O3 experiments. A linear relationship between particle concentration and I2 was found, in reasonable agreement with previous studies. Scaling the laboratory relationship to aerosol concentrations typical of the coastal boundary layer suggests a I2 mixing ratio range of 6−93 pptv can account for the observed particle production events. Aerosol number concentration produced from I2 is more than a factor of 10 higher than thatproduced from CH2I2 for the same mixing ratios

Developing Metrics for Novel Value-Added Products: The Case of Hemp in Vermont

Vermont Farm to Plate 2020 identifies hemp as one of ten emergent agricultural products critical for Vermont’s future and has made recommendations for investments in hemp research, education, feasibility, and innovation programs. These investments are essential to develop niche food, feed, fiber, and industrial products, professionals, and markets that go “beyond CBD” (VFP, 2020). This project develops indicators for an important, value added budding crop in Vermont: hemp. For the purposes of this white paper, indicators are “a way to measure, indicate or point to with more or less exactness,” or “something used to show the condition of a system” (Feenstra et al., 2005). We focus on the example of hemp to illustrate how emerging value-added crops contribute to sustainable food systems. We use a set of design principles to ensure the applicability of developed indicators for decision making. This framework, its processes, and measures, are transferable to any nascent crop for evaluating economic, environmental, and social sustainability. Our objectives are to: Develop a set of indicators to measure the economic, social, and environmental inputs of hemp in Vermont. Identify techniques and data sources for mining hemp metrics. Evaluate the hierarchical levels of mined data and transecting indicators to inform growing discussions of metric integration and forecasting agricultural food sustainability. Our approach is grounded in the FAO food systems model, Doughnut Economics, which uses the UN Sustainable Development Goals as a foundation to describe “social floors” and planetary boundaries, and the concepts of seven community capitals: political, cultural, human, social, financial, built, and environmental. Our work plan included a two-day virtual workshop with required reading prior to the event, and involved both University researchers and stakeholders representing production, industry, finance, government, and NGOs. We describe 35 metrics to assess the sustainability of hemp in the Vermont economy, environment and community going forward (Figure 2 and Table 1). We also make several recommendations to move the collection of food system metrics forward. General recommendations include: Farmer surveys to specifically address challenges facing farmers growing a novel crop. In hemp this is particularly important, as the crop attracts many who are new to farming, and no one has been able to legally grow it at field scale in the US for the past several generations. Community/consumer surveys to collect data on community needs and impacts of novel value added crops (hemp) Collection and curation of spatial data tied to appropriate metrics Allocation of ARS funds for at least one data professional with skills across data types and methods, including individual, spatial, community level, etc. Funds to build out nutrient mass balance and soil carbon stock models for different soil types and production approaches The use of a grower-friendly tracking App with incentives (GoCrop) Development of a dashboard to easily visualize direction and degree of movement toward a desired state We make more specific recommendations in the Appendix where each metric is described in detail