Post-treatment changes in permanent retention

OBJECTIVES While permanent retention is today the method of choice to stabilize orthodontic treatment outcomes, recent studies have increasingly reported posttreatment changes in tooth position during permanent retention. We conducted this study to analyze changes in the anterior mandible, whether the changes follow an underlying movement pattern, and, aiming for a preventive strategy, whether any risk factors could be identified comparing findings with the pretreatment situations. METHODS We included 30 patients who had worn fixed Twistflex retainers (UK 3-3) extending from canine to canine in the mandible. Casts reflecting the intraoral situations before orthodontic treatment (T0), directly after completion of active therapy (T1), and 6 months later (T2) were scanned and superimposed using Imageware Surfacer software. Posttreatment changes (T2-T1) of tooth position within the retainer block were analyzed on 3D virtual models and were compared to pretreatment (T0) and treatment-related (T1-T0) findings to identify potential risk factors. RESULTS Almost all analyzed patients revealed three-dimensional changes in tooth position within the retainer block. Comparing these movements, we repeatedly found rotated retainer blocks in labio-oral direction, while the center of rotation was located at the first incisors. This pattern was associated with intercanine expansion and excessive overjet correction during orthodontic treatment. The canines underwent the most pronounced (rotational and translational) movements. CONCLUSIONS In general permanent lingual retainers are safe but in special clinical cases retainers can induce undesired tooth movement. Risk factors seem to be intercanine expansion and excessive overjet correction during orthodontic treatment. In specific cases an additional retention device might be needed

Failure patterns of different bracket systems and their influence on treatment duration: A retrospective cohort study

OBJECTIVES To compare the failure pattern of four different bracket types and to assess its effect on treatment duration. MATERIALS AND METHODS A total of 78 white patients (28 male, 50 female) with a mean age of 12.6 years were included in this retrospective cohort study and treated for a mean period of 30.6 months. The patients were treated in a private practice with stainless steel conventionally ligated brackets, ceramic conventionally ligated brackets, stainless steel self-ligating brackets, or nickel-free self-ligating brackets. The loss of at least one bracket during the course of treatment was analyzed with Cox proportional hazards survival analyses and generalized linear regression. RESULTS The overall bracket failure rate at the tooth level was 14.1% (217 brackets), with significant differences according to tooth type (between 8.0%-23.4%) and bracket type (between 11.2%-20.0%). After taking confounders into account, patients treated with ceramic brackets lost more brackets (hazard ratio = 1.62; 95% confidence interval = 1.14-2.29; P = .007) than patients with stainless steel brackets. On average, treatment time increased by 0.6 months (95% confidence interval = 0.21-1.05; P = .004) for each additional failed bracket. CONCLUSIONS Bracket failure was more often observed with ceramic brackets and was associated with increased treatment duration

The influence of enamel sandblasting on the shear bond strength and fractography of the bracket-adhesive-enamel complex tested in vitro by the DIN 13990:2017-04 standard

OBJECTIVES This study was conducted in order to investigate whether enamel sandblasting as an adjunct or substitute to the acid-etch technique has an effect on the shear bond strength (SBS) and fractography of the bracket-adhesive-enamel complex using the DIN 13990:2017-04 standard. MATERIALS AND METHODS Upper central incisor brackets (discovery®, Dentaurum, Germany) were bonded using Transbond XT™ (3M Unitek, Germany) on bovine incisors prepared by four different methods (15 samples each): sandblasting with 27 μm AlO at 1.2 bar (s), acid etching with 37.4% phosphoric acid (a), sandblasting with 27 μm AlO at 1.2 bar followed by acid etching (s1a), and sandblasting with 50 μm AlO at 5.7 bar followed by acid etching (s2a). The SBS and adhesive remnant index (ARI) were measured, followed by one-way analysis of variance and Fisher's exact tests at 5%. RESULTS The SBS in groups s (5.6 ± 2.2 MPa), a (17.1 ± 4.3 MPa), s1a (18.3 ± 4.3 MPa), and s2a (18.5 ± 4.6 MPa) indicated that the s group was significantly inferior to all the other groups (p < 0.001). Likewise, the ARI analysis indicated a different performance of the s group (mostly ARI of 0) compared to the other groups (p < 0.001) and a tendency for different ARI between the a and s1a/s2a groups. CONCLUSIONS In vitro enamel sandblasting could not substitute acid etching and did not offer improved SBS when used before acid etching, regardless of air pressure and powder granulation. Sandblasting without acid etching produced less residual resin on the tooth after debonding. CLINICAL RELEVANCE The clinical use of adjunct enamel sandblasting prior to etching to enhance SBS has to be questioned

In vitro cyclic shear fatigue of the bracket-adhesive complex: a pilot study

OBJECTIVE: The aim of this study was to describe the effect of fatigue on the shear strength of the bracket-adhesive complex. MATERIALS AND METHODS: Brackets with laser-structured (Discovery®, Dentaurum) and foil mesh bases (Ultra-Minitrim®, Dentaurum) were bonded onto silanized stainless steel flat plates with two chemically curing adhesives (No-Mix Bonding System, Dentaurum; Concise™, 3M Unitek) and aged in distilled water at 37°C for 3 days. One group of specimens was used to determine shear-bond strength. The second group underwent shear-fatigue testing with a testing machine (Zwick 1445) according to the staircase method for 1,000 cycles, and the surviving specimens were subjected to shear-strength testing. The shear strength of the fatigued and non-fatigued specimens were then compared. RESULTS: Fatigued specimens demonstrated an 8% gain in shear strength in material group A (Discovery®/No-Mix) and a 10% loss of shear strength in material group D (Ultra-Minitrim®/Concise™) compared to the non-fatigued group. We observed no statistically significant differences in material groups B (Ultra-Minitrim®/No-Mix) and C (Discovery®/Concise™). The fatigue ratio varied between 60% and 67%. Among the non-fatigued specimens, the bracket Discovery® with laser-structured base showed circa 59% greater shear strength than the foil-mesh bracket Ultra-Minitrim®, and the adhesive Concise™ about 66% more shear strength than the No-Mix. CONCLUSION: Shear fatigue of the bracket-adhesive complex for 1,000 cycles revealed a variable, material-dependent influence on the shear strength of the bracket-adhesive complex. However, the effect of fatigue on shear strength was less significant than the effect of other factors such as bracket and adhesive type

Candidate Genes for Nonsyndromic Cleft Palate Detected by Exome Sequencing

Nonsyndromic cleft palate only (nsCPO) is a facial malformation that has a livebirth prevalence of 1 in 2,500. Research suggests that the etiology of nsCPO is multifactorial, with a clear genetic component. To date, genome-wide association studies have identified only 1 conclusive common variant for nsCPO, that is, a missense variant in the gene grainyhead-like-3 ( GRHL3). Thus, the underlying genetic causes of nsCPO remain largely unknown. The present study aimed at identifying rare variants that might contribute to nsCPO risk, via whole-exome sequencing (WES), in multiply affected Central European nsCPO pedigrees. WES was performed in 2 affected first-degree relatives from each family. Variants shared between both individuals were analyzed for their potential deleterious nature and a low frequency in the general population. Genes carrying promising variants were annotated for 1) reported associations with facial development, 2) multiple occurrence of variants, and 3) expression in mouse embryonic palatal shelves. This strategy resulted in the identification of a set of 26 candidate genes that were resequenced in 132 independent nsCPO cases and 623 independent controls of 2 different ethnicities, using molecular inversion probes. No rare loss-of-function mutation was identified in either WES or resequencing step. However, we identified 2 or more missense variants predicted to be deleterious in each of 3 genes ( ACACB, PTPRS, MIB1) in individuals from independent families. In addition, the analyses identified a novel variant in GRHL3 in 1 patient and a variant in CREBBP in 2 siblings. Both genes underlie different syndromic forms of CPO. A plausible hypothesis is that the apparently nonsyndromic clefts in these 3 patients might represent hypomorphic forms of the respective syndromes. In summary, the present study identified rare variants that might contribute to nsCPO risk and suggests candidate genes for further investigation

Genome-wide meta-analyses of nonsyndromic cleft lip with or without cleft palate identify six new risk loci

We have conducted the first meta-analyses for nonsyndromic cleft lip with or without cleft palate (NSCL/P) using data from the two largest genome-wide association studies published to date. We confirmed associations with all previously identified loci and identified six additional susceptibility regions (1p36, 2p21, 3p11.1, 8q21.3, 13q31.1 and 15q22). Analysis of phenotypic variability identified the first specific genetic risk factor for NSCLP (nonsyndromic cleft lip plus palate) (rs8001641; P(NSCLP) = 6.51 × 10(-11); homozygote relative risk = 2.41, 95% confidence interval (CI) 1.84-3.16)