Laser-generated surface acoustic wave-based study and detection of surface cracks

Monitoring cracks to check the integrity of engineering materials by Non- Destructive Testing (NDT) in industry is significant in industry. And within the NDT techniques, Laser-Generated Surface Acoustic Wave technique (LSAW) has shown to be a promising technique. To further develop non-contact and accurate testing strengths of this method, models for analyzing the generation, propagation and tracking of surface acoustic waves (SAW’s) changes in S45C steel samples with distributed cracks are developed by using Finite Element Method (FEM). Time and frequency domain analyses are used to process the acoustic wave signals after the interaction with cracks. The simulation results and preliminary analyses reveal the good potential LSAW’s have to monitor cracks. First results in developing an experimental setup for crack detection are also provided

Development of a Hybrid Atomic Force Microscopic Measurement System Combined with White Light Scanning Interferometry

A hybrid atomic force microscopic (AFM) measurement system combined with white light scanning interferometry for micro/nanometer dimensional measurement is developed. The system is based on a high precision large-range positioning platform with nanometer accuracy on which a white light scanning interferometric module and an AFM head are built. A compact AFM head is developed using a self-sensing tuning fork probe. The head need no external optical sensors to detect the deflection of the cantilever, which saves room on the head, and it can be directly fixed under an optical microscopic interferometric system. To enhance the system’s dynamic response, the frequency modulation (FM) mode is adopted for the AFM head. The measuring data can be traceable through three laser interferometers in the system. The lateral scanning range can reach 25 mm × 25 mm by using a large-range positioning platform. A hybrid method combining AFM and white light scanning interferometry is proposed to improve the AFM measurement efficiency. In this method, the sample is measured firstly by white light scanning interferometry to get an overall coarse morphology, and then, further measured with higher resolution by AFM. Several measuring experiments on standard samples demonstrate the system’s good measurement performance and feasibility of the hybrid measurement method

Comparative Study of Adenosine Analogs as Inhibitors of Protein Arginine Methyltransferases and a Clostridioides difficile- Specific DNA Adenine Methyltransferase

S-Adenosyl-l-methionine (SAM) analogs are adaptable tools for studying and therapeutically inhibiting SAM-dependent methyltransferases (MTases). Some MTases play significant roles in host–pathogen interactions, one of which is Clostridioides difficile-specific DNA adenine MTase (CamA). CamA is needed for efficient sporulation and alters persistence in the colon. To discover potent and selective CamA inhibitors, we explored modifications of the solvent-exposed edge of the SAM adenosine moiety. Starting from the two parental compounds (6e and 7), we designed an adenosine analog (11a) carrying a 3-phenylpropyl moiety at the adenine N6-amino group, and a 3-(cyclohexylmethyl guanidine)-ethyl moiety at the sulfur atom off the ribose ring. Compound 11a (IC50 = 0.15 μM) is 10× and 5× more potent against CamA than 6e and 7, respectively. The structure of the CamA–DNA–inhibitor complex revealed that 11a adopts a U-shaped conformation, with the two branches folded toward each other, and the aliphatic and aromatic rings at the two ends interacting with one another. 11a occupies the entire hydrophobic surface (apparently unique to CamA) next to the adenosine binding site. Our work presents a hybrid knowledge-based and fragment-based approach to generating CamA inhibitors that would be chemical agents to examine the mechanism(s) of action and therapeutic potentials of CamA in C. difficile infection

Systematic Design of Adenosine Analogs as Inhibitors of a Clostridioides difficile- Specific DNA Adenine Methyltransferase Required for Normal Sporulation and Persistence

Antivirulence agents targeting endospore-transmitted Clostridioides difficile infections are urgently needed. C. difficile-specific DNA adenine methyltransferase (CamA) is required for efficient sporulation and affects persistence in the colon. The active site of CamA is conserved and closely resembles those of hundreds of related S-adenosyl-l-methionine (SAM)-dependent methyltransferases, which makes the design of selective inhibitors more challenging. We explored the solvent-exposed edge of the SAM adenosine moiety and systematically designed 42 analogs of adenosine carrying substituents at the C6-amino group (N6) of adenosine. We compare the inhibitory properties and binding affinity of these diverse compounds and present the crystal structures of CamA in complex with 14 of them in the presence of substrate DNA. The most potent of these inhibitors, compound 39 (IC50 ∼ 0.4 μM and KD ∼ 0.2 μM), is selective for CamA against closely related bacterial and mammalian DNA and RNA adenine methyltransferases, protein lysine and arginine methyltransferases, and human adenosine receptors

Proceedings of the Thirteenth International Society of Sports Nutrition (ISSN) Conference and Expo

Meeting Abstracts: Proceedings of the Thirteenth International Society of Sports Nutrition (ISSN) Conference and Expo Clearwater Beach, FL, USA. 9-11 June 201

Technical Note: Evaluation of audiovisual biofeedback smartphone application for respiratory monitoring in radiation oncology

PurposeRadiation dose delivered to targets located near the upper abdomen or thorax are significantly affected by respiratory motion, necessitating large margins, limiting dose escalation. Surrogate motion management devices, such as the Real-time Position Management (RPM™) system (Varian Medical Systems, Palo Alto, CA), are commonly used to improve normal tissue sparing. Alternative to current solutions, we have developed and evaluated the feasibility of a real-time position management system that leverages the motion data from the onboard hardware of Apple iOS devices to provide patients with visual coaching with the potential to improve the reproducibility of breathing as well as improve patient compliance and reduce treatment delivery time.Methods and materialsThe iOS application, coined the Instant Respiratory Feedback (IRF) system, was developed in Swift (Apple Inc., Cupertino, CA) using the Core-Motion library and implemented on an Apple iPhone® devices. Operation requires an iPhone®, a three-dimensional printed arm, and a radiolucent projector screen system for feedback. Direct comparison between IRF, which leverages sensor fusion data from the iPhone®, and RPM™, an optical-based system, was performed on multiple respiratory motion phantoms and volunteers. The IRF system and RPM™ camera tracking marker were placed on the same location allowing for simultaneous data acquisition. The IRF surrogate measurement of displacement was compared to the signal trace acquired using RPM™ with univariate linear regressions and Bland-Altman analysis.ResultsPeriodic motion shows excellent agreement between both systems, and subject motion shows good agreement during regular and irregular breathing motion. Comparison of IRF and RPM™ show very similar signal traces that were significantly related across all phantoms, including those motion with different amplitude and frequency, and subjects' waveforms (all r > 0.9, P < 0.0001). We demonstrate the feasibility of performing four-dimensional cone beam computed tomography using IRF which provided similar image quality as RPM™ when reconstructing dynamic motion phantom images.ConclusionsFeasibility of an iOS application to provide real-time respiratory motion is demonstrated. This system generated comparable signal traces to a commercially available system and offers an alternative method to monitor respiratory motion

Parameters effects study on pulse laser for the generation of Surface Acoustic Waves in human skin detection applications

Dante J. (MEF Author)##nofulltext##Laser-induced Surface Acoustic Waves (LSAWs) has been promisingly and widely used in recent years due to its rapid, high accuracy and non-contact evaluation potential of layered and thin film materials. For now, researchers have applied this technology on the characterization of materials' physical parameters, like Young's Modulus, density, and Poisson's ratio; or mechanical changes such as surface cracks and skin feature like a melanoma. While so far, little research has been done on providing practical guidelines on pulse laser parameters to best generate SAWs. In this paper finite element simulations of the thermos-elastic process based on human skin model for the generation of LSAWs were conducted to give the effects of pulse laser parameters have on the generated SAWs. And recommendations on the parameters to generate strong SAWs for detection and surface characterization without cause any damage to skin are given.WOS:000364657700035Scopus - Affiliation ID: 60105072Conference Proceedings Citation Index- ScienceProceedings PaperMayıs2015YÖK - 2014-1

Simulation study and guidelines to generate Laser-induced Surface Acoustic Waves for human skin feature detection

Dante, J.Dorantes-Gonzalez (MEF Author)Despite the seriously increasing number of people contracting skin cancer every year, limited attention has been given to the investigation of human skin tissues. To this regard, Laser-induced Surface Acoustic Wave (LSAW) technology, with its accurate, non-invasive and rapid testing characteristics, has recently shown promising results in biological and biomedical tissues. In order to improve the measurement accuracy and efficiency of detecting important features in highly opaque and soft surfaces such as human skin, this paper identifies the most important parameters of a pulse laser source, as well as provides practical guidelines to recommended proper ranges to generate Surface Acoustic Waves (SAWS) for characterization purposes. Considering that melanoma is a serious type of skin cancer, we conducted a finite element simulation-based research on the generation and propagation of surface waves in human skin containing a melanoma-like feature, determine best pulse laser parameter ranges of variation, simulation mesh size and time step, working bandwidth, and minimal size of detectable melanoma.WOS:000361251700004Scopus - Affiliation ID: 60105072Science Citation Index ExpandedQ1 - Q2ArticleUluslararası işbirliği ile yapılan - EVETAralık2015YÖK - 2015-1