Gene expression profiling in Saccharomyces cerevisiae grown at different specific gravity environments

The global gene expression profiles of industrial strains of Saccharomyces cerevisiae responding to nitrogen deficiency and very high sugar concentrations stresses were determined by oligonucleotide microarray analysis of ~ 6200 yeast open reading frames. Genomics analysis showed that 400 genes in S. cerevisiae was differentially expressed by more than 1.5-fold compared with controls at late-logarithmic phase of fermentation, as the yeast adapted to changing nutritional, environmental and physiological conditions. The genes of many pathways are regulated in a highly coordinated manner. The repressed expression of GDH1 and up-regulation of ARO10 within the contrast of Q270/Q10 indicated high energy demanding of yeast cells under high sugar stress. Activities of G3P shuttle indicated that under very high gravity environment, sufficient assimilatory nitrogen enhances yeast’s ability of redox balancing, and therefore higher stress-tolerance and higher fermentation efficiency of yeast. Under contrast W270/Q270, the up-regulation of DUR1,2 responsible for urea degradation induces the glutamate biosynthesis and the consumption of -ketoglutarate. This may indicate that higher nitrogen level would enable higher activities in the TCA cycle, and therefore generate more energy for biosynthesis and yeast cell proliferation under very high gravity fermentation conditions. Nitrogen metabolism was also stimulated by high nitrogen level when yeast was grown in very high gravity environment

Elastic Valley Spin Controlled Chiral Coupling in Topological Valley Phononic Crystals

Distinct from the phononic valley pseudo-spin, the real physical spin of elastic waves adds a novel tool-kit capable of envisaging the valley-spin physics of topological valley phononic crystals from a local viewpoint. Here, we report the observation of local elastic valley spin as well as the hidden elastic spin-valley locking mechanism overlooked before. We demonstrate that the selective one-way routing of valley phonon states along the topological interface can be reversed by imposing the elastic spin meta-source at different interface locations with opposite valley-spin correspondence. We unveil the physical mechanism of selective directionality as the elastic spin controlled chiral coupling of valley phonon states, through both analytical theory and experimental measurement of the opposite local elastic spin density at different interface locations for different transport directions. The elastic spin of valley topological edge phonons can be extended to other topological states and offers new tool to explore topological metamaterials.Comment: 5 pages, 3 figures, of main text + supplementary 10 figures. To be published in Phys. Rev. Let

Conduction modulation of solution-processed two-dimensional materials

Solution-processed two-dimensional (2D) materials hold promise for their scalable applications. However, the random, fragmented nature of the solution-processed nanoflakes and the poor percolative conduction through their discrete networks limit the performance of the enabled devices. To overcome the problem, we report conduction modulation of the solution-processed 2D materials via the Stark effect. Using liquid-phase exfoliated molybdenum disulfide (MoS2) as an example, we demonstrate nonlinear conduction modulation with a switching ratio of >105 by the local fields from the interfacial ferroelectric P(VDF-TrFE). Through density-functional theory calculations and in situ Raman scattering and photoluminescence spectroscopic analysis, we understand the modulation arises from a charge redistribution in the solution-processed MoS2. Beyond MoS2, we show the modulation may be viable for the other solution-processed 2D materials and low-dimensional materials. The effective modulation can open their electronic device applications

Variations in species diversity patterns and community assembly rules among vegetation types in the karst landscape

The various vegetation types in the karst landscape have been considered the results of heterogeneous habitats. However, the lack of a comprehensive understanding of regional biodiversity patterns and the underlying ecological processes limits further research on ecological management. This study established forest dynamic plots (FDPs) of the dominant vegetation types (shrubland, SL; mixed tree and shrub forest, MTSF; coniferous forest, CF; coniferous broadleaf mixed forest, CBMF; and broadleaf forest, BF) in the karst landscape and quantified the species diversity patterns and potential ecological processes. The results showed that in terms of diversity patterns, the evenness and species richness of the CF community were significantly lower than other vegetation types, while the BF community had the highest species richness. The other three vegetation types showed no significant variation in species richness and evenness. However, when controlling the number of individuals of FDPs, the rarefied species richness showed significant differences and ranked as BF > SL > MTSF > CBMF > CF, highlighting the importance of considering the impacts of abundance. Additionally, the community assembly of climax communities (CF or BF) was dominated by stochastic processes such as species dispersal or species formation, whereas deterministic processes (habitat filtering) dominated the secondary forests (SL, MTSF, and CBMF). These findings proved that community assembly differs mainly between the climax community and other communities. Hence, it is crucial to consider the biodiversity and of the potential underlying ecological processes together when studying regional ecology and management, particularly in heterogeneous ecosystems

Numerical study on energy and exergy performances of a microencapsulated phase change material slurry based photovoltaic/thermal module

Microencapsulated phase change material (MPCM) slurry has proven to have potential in elevating the overall performance of a photovoltaic/thermal (PV/T) module as a working fluid. In order to make full use of the superiority of MPCM slurry and further improve energy and exergy efficiencies of the PV/T module, the effects of MPCM concentration and melting temperature under a wide inlet fluid velocity range were explored based on a three-dimensional numerical model of coupled heat transfer in this study. The results show that both the energy and exergy efficiencies increased with the concentration. A lower melting temperature resulted in higher energy efficiency, whereas a higher melting temperature is helpful for exergy efficiency improvement. The slurry with an excessively low melting temperature (e.g. 27 °C) even led to lower exergy efficiency than pure water. The melting temperature needs to be precisely tailored to make a compromise between energy and exergy efficiencies. In comparison with pure water, the improvement in energy efficiency provided by the slurry was further enhanced at a lower inlet velocity, while the improvement in exergy efficiency was optimized by adjusting the inlet velocity to a certain value. The maximum improvement in energy efficiency provided by the slurry was 8.3%, whilst that in exergy efficiency was 3.23% in this work. From the above, the superiority of MPCM slurry can be further promoted by selecting suitable material properties and operating parameters.The authors would like to acknowledge the financial support of the Engineering and Physical Sciences Research Council (EPSRC) of the United Kingdom (Grant Nos. EP/N000714/1 and EP/N021142/1), National Natural Science Foundation of China (Grant Nos. 51606135 and 51776142) and Natural Science Foundation of Hubei Province (Grant No. 2016CFB156)

Forest plots of hazard ratios (HRs) and 95% confidence intervals (95% CIs) for event-free survival endpoints in DLBCL patients with C-MYC gene abnormalities (A), overexpression of C-MYC mRNA (B) and C-MYC protein (C).

<p>Squares represent the HR of each study, and the area of each square was proportional to the weight of each study in the meta-analysis; Horizontal lines, 95% CIs; Closed diamond, pooled HRs with their 95% CIs.</p

C-MYC Aberrations as Prognostic Factors in Diffuse Large B-cell Lymphoma: A Meta-Analysis of Epidemiological Studies

<div><p>Objectives</p><p>Various studies have investigated the prognostic value of C-MYC aberrations in diffuse large B-cell lymphoma (DLBCL). However, the role of C-MYC as an independent prognostic factor in clinical practice remains controversial. A systematic review and meta-analysis were performed to clarify the clinical significance of C-MYC aberrations in DLBCL patients.</p><p>Methods</p><p>The pooled hazard ratios (HRs) for overall survival (OS) and event-free survival (EFS) were calculated as the main effect size estimates. The procedure was conducted according to the Cochrane handbook and PRISMA guidelines, including the use of a heterogeneity test, publication bias assessment, and meta-regression, as well as subgroup analyses.</p><p>Results</p><p>Twenty-four eligible studies enrolling 4662 patients were included in this meta-analysis. According to the nature of C-MYC aberrations (gene, protein, and mRNA), studies were divided into several subgroups. For DLBCL patients with C-MYC gene abnormalities, the combined HR was 2.22 (95% confidence interval, 1.89 to 2.61) for OS and 2.29 (95% confidence interval, 1.81 to 2.90) for EFS, compared to patients without C-MYC gene abnormalities. For DLBCL patients with overexpression of C-MYC protein and C-MYC mRNA, pooled HRs for OS were 2.13 and 1.62, respectively. C-MYC aberrations appeared to play an independent role among other well-known prognostic factors in DLBCL. Addition of rituximab could not overcome the inferior prognosis conferred by C-MYC.</p><p>Conclusion</p><p>The present systematic review and meta-analysis confirm the prognostic value of C-MYC aberrations. Screening of C-MYC should have definite prognostic meaning for DLBCL stratification, thus guaranteeing a more tailored therapy.</p></div