Genome sequence of the acid-tolerant Desulfovibrio sp. DV isolated from the sediments of a Pb-Zn mine tailings dam in the Chita region, Russia

Here we report the draft genome sequence of the acid-tolerant Desulfovibrio sp. DV isolated from the sediments of a Pb-Zn mine tailings dam in the Chita region, Russia. The draft genome has a size of 4.9 Mb and encodes multiple K+-transporters and proton-consuming decarboxylases. The phylogenetic analysis based on concatenated ribosomal proteins revealed that strain DV clusters together with the acid-tolerant Desulfovibrio sp. TomC and Desulfovibrio magneticus. The draft genome sequence and annotation have been deposited at GenBank under the accession number MLBG00000000

Genome sequence of the copper resistant and acid-tolerant Desulfosporosinus sp. BG isolated from the tailings of a molybdenum-tungsten mine in the Transbaikal area

Here, we report on the draft genome of a copper-resistant and acidophilic Desulfosporosinus sp. BG, isolated from the tailings of a molybdenum-tungsten mine in Transbaikal area. The draft genome has a size of 4.52 Mb and encodes transporters of heavy metals. The phylogenetic analysis based on concatenated ribosomal proteins revealed that strain BG clusters together with the other acidophilic copper-resistant strains Desulfosporosinus sp. OT and Desulfosporosinus sp. I2. The K+-ATPase, Na+/H+ antiporter and amino acid decarboxylases may participate in enabling growth at low pH. The draft genome sequence and annotation have been deposited at GenBank under the accession number NZ_MASS00000000

Genome sequence of the acid-tolerant Desulfovibrio sp. DV isolated from the sediments of a Pb-Zn mine tailings dam in the Chita region, Russia

Here we report the draft genome sequence of the acid-tolerant Desulfovibrio sp. DV isolated from the sediments of a Pb-Zn mine tailings dam in the Chita region, Russia. The draft genome has a size of 4.9 Mb and encodes multiple K+-transporters and proton-consuming decarboxylases. The phylogenetic analysis based on concatenated ribosomal proteins revealed that strain DV clusters together with the acid-tolerant Desulfovibrio sp. TomC and Desulfovibrio magneticus. The draft genome sequence and annotation have been deposited at GenBank under the accession number MLBG00000000

Genome sequence of the copper resistant and acid-tolerant Desulfosporosinus sp. BG isolated from the tailings of a molybdenum-tungsten mine in the Transbaikal area

Here, we report on the draft genome of a copper-resistant and acidophilic Desulfosporosinus sp. BG, isolated from the tailings of a molybdenum-tungsten mine in Transbaikal area. The draft genome has a size of 4.52 Mb and encodes transporters of heavy metals. The phylogenetic analysis based on concatenated ribosomal proteins revealed that strain BG clusters together with the other acidophilic copper-resistant strains Desulfosporosinus sp. OT and Desulfosporosinus sp. I2. The K+-ATPase, Na+/H+ antiporter and amino acid decarboxylases may participate in enabling growth at low pH. The draft genome sequence and annotation have been deposited at GenBank under the accession number NZ_MASS00000000

Selection of a microbial community in the course of formation of acid mine drainage

Changes in microbial community composition during formation of an acid mine drainage were studied on a model of two water reservoirs located in the Ozernoye open-cast mine for polymetallic ores in Eastern Siberia. The first reservoir, Bu-18, was filled with groundwater, had a neutral pH and low levels of sulfate and dissolved metal ions. The second reservoir, Bu-16, was an acid mine drainage (pH 2.85) filled with the water from Bu-18, which passed through rocks containing sulfide minerals. The Bu-16 water contained 1405 mg/L of sulfate, 164 mg/L of manganese, 78 mg/L of magnesium, and 26 mg/L of iron. Molecular analysis of the microbial communities of two reservoirs, carried out using high-throughput sequencing of the 16S rRNA gene fragments, showed that formation of the acid mine drainage was accompanied by a decrease in microbial diversity and by selection of several dominant taxonomic and functional groups. Chemolithoautotrophic iron- and sulfur-oxidizing bacteria of the genera Leptospirillum, Acidithiobacillus, Gallionella, Sulfuriferula, and Sulfobacillus constituted most of the prokaryotic community in Bu-16. Heterotrophic bacteria of the genera Ferrimicrobium and Metallibacterium, capable of reducing Fe(III) under anaerobic conditions, were present as minor components. Over 20% of the community were members of the Candidate Phyla Radiation group and of the candidate phylum Dependentiae (TM6), known for their parasitic or symbiotic lifestyle. These groups of bacteria were rarely found in acid mine drainage and only in minor quantities. Potential hosts of the Dependentiae, flagellates of the genus Spumella, were found among eukaryotes in Bu-16

Isolation of new thermophilic sulfidogens from microbial mat associated with groundwater discharge in the Tunkin valley

Spore-forming sulfate-reducing bacteria, previously assigned to the genus Desulfotomaculum, are typical inhabitants of thermal groundwater biotopes (Aüllo et al., 2013; Frank et al., 2016). Due to their ability to form spores, to grow autotrophically, and to survive high temperatures, this group is a successful colonizer of groundwaters. Revision of the genus Desulfotomaculum resulted in description of four new genera, Desulfallas, Desulfofundulus, Desulfofarcimen, and Desulfohalotomaculum (Watanabe et al., 2018