Personality and social education and its influence on the classroom climate

This work is focused on the topic of school class climate and its enhancement by the methods of personality and social education in a concrete class where the author is working as a teacher. The theoretical part is dedicated to the class climate and its characteristics. It describes what influences, by which methods it can be investigated and what can it be influenced by. In the practical part the author uses the methods of the teacher's active research to investigate the climate in her own class and also self-reflexively examines herself from the view of the beginning teacher. During the time period of one school year continually monitors the climate in her class and states the most effective ways towards improvement. The results of the research show that the methods of personality and social education have an influence to the class climate if they are used in a natural and casual manner. KEY WORDS: Class climate, teacher's active research, beginning teacher, social skills, self - reflection, begin together, personality and social education, cooperation, relations, pre - puberty, problematic pupil, method

Intercompany mentoring

The diploma thesis is dedicated to the intercompany mentoring issue, its thorough description and specific practical application in the company. The objectives are to define the intercompany mentoring as a new form of corporate learning, to analyze it in detail and to assess its benefits to the certain company. Its strengths / weaknesses and the possible opportunities and threats are found in the theoretical part via SWOT analysis. The assessment of intercompany mentoring is based on the interviews with mentors, mentees and last but not least with mentees' superiors. These semi-structured interviews are focused on finding of the strengths / weaknesses and the opportunities or threats. It means that the SWOT analysis is used also in the practical part of the thesis. The output of the thesis is the final resume and my personal improvement proposals. The results of the thesis can help those companies that are looking for new ways to develop their employees or which are hesitating to participate in the intercompany mentoring. KEYWORDS Learning, development, intercompany, mentoring, mentor, mentee, HR, coordinator, company culture, code of ethic

MOESM4 of Protein aggregation and membrane lipid modifications under lactic acid stress in wild type and OPI1 deleted Saccharomyces cerevisiae strains

Additional file 4: Figure S4. Second derivatives of the FTIR absorption spectra of S. cerevisiae BY4741 and opi1Δ cells, in the absence of lactic acid: exponential phase. Cells were grown in shake flasks in minimal (YNB) medium with 2 % w/v glucose at pH3. FTIR analysis was performed at 18 h after the inoculation, corresponding to the exponential phase of growth. a: amide I band; b: vibrational modes mainly due to lipid CH2/CH3 and to phosphate groups, c: stretching modes from lipid hydrocarbon tails. In a and b second derivative spectra have been normalized to the tyrosine band at ~ 1516 cm−1, while in c spectra have been normalized at the CH3 band at ~ 2959 cm−1

MOESM5 of Protein aggregation and membrane lipid modifications under lactic acid stress in wild type and OPI1 deleted Saccharomyces cerevisiae strains

Additional file 5: Figure S5. Second derivatives of the FTIR absorption spectra of S. cerevisiae BY4741 and opi1Δ cells, in the presence of lactic acid: exponential phase. Cells were grown in shake flasks in minimal (YNB) medium with 2 % w/v glucose in the presence of 46 g/L of lactic acid (LA) at pH3. FTIR analysis was performed at 18 h after the inoculation, corresponding to the exponential phase of growth. a: amide I band; b: vibrational modes mainly due to lipid CH2/CH3 and to phosphate groups, c: stretching modes from lipid hydrocarbon tails. In a and b second derivative spectra have been normalized to the tyrosine band at ~ 1516 cm−1, while in c spectra have been normalized at the CH3 band at ~ 2959 cm−1

List of yeast strains constructed and used in this study

<p>List of yeast strains constructed and used in this study</p

Growth curves of wild type and L-ascorbic acid producing yeasts under oxidative stress.

<p>Kinetics of growth of wild type and engineered strains GRF18U (left panels) and BY4742 (right panels) as inoculated in minimal glucose media without H₂O₂ (3A and 3B) or in presence of H₂O₂ 3.5 mM (3C and 3D). • GRF18U wild type; ▪ GRF18U[<i>ScALO AtLGDH AtME AtMIP</i>]; ▴ GRF18U[<i>ScALO AtLGDH AtME AtMIP RnFGT</i>]; ○ BY4742 wild type; □ BY4742[<i>ScALO AtLGDH AtME AtMIP</i>]; ▵ BY4742[<i>ScALO AtLGDH AtME AtMIP RnFGT</i>].</p

List of expression plasmids constructed and used in this study*

<p>Abbreviations: Sc: <i>Saccharomyces cerevisiae</i>; At: <i>Arabidopsis thaliana</i>; Rn: <i>Rattus norvegicus</i>; Zb: <i>Zygosaccharomyces bailii</i>;</p><p>TPI: Triose Phosphate Isomerase</p><p><i>URA3, HIS3, LEU2, LYS2</i>: gene markers conferring growth to auxotrophic yeast strains in the absence of uracil, histidine, leucine and lysine, respectively.</p><p>Nat^R: cassette conferring resistance to nourseotricine.</p><p>Kan^R: cassette conferring resistance to Geneticin.</p><p>CEN and INT: centromeric and integrative plasmids, respectively.</p>*<p>a complete description of plasmids construction is given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001092#s4" target="_blank">Materials and Methods</a></p

Flow cytometric analysis of wild type and vitamin C producing yeasts under oxidative stress.

<p>Panel 5A: schematic representation of the different subpopulations that can be observed in the following panels, where wild type (5B) and recombinant strains (5C and 5D) grown in minimal glucose medium added with H₂O₂ 3.0 mM were analyzed after DHR123 and PI staining (rodamine signal is reported in the abscissa and PI signal on the ordinate axes). Upper panels: GRF18U background. Lower panels: BY background. (B): wild type. (C): [<i>ScALO AtLGDH AtME AtMIP</i>] transformed cells. (D): [<i>ScALO AtLGDH AtME AtMIP RnFGT</i>] transformed cells</p

Conversion of D-Glucose into L-ascorbic acid (milligrams/liter/OD) by transformed <i>S. cerevisiae</i> GRF18U and BY4742 cells.

<p>All strains were grown on mineral medium (2% w/v glucose, 0.67% w/v YNB), starting with an initial OD660 of 0.05 for 18 h, when samples were taken and the concentration of L-ascorbic acid inside the cells was determined (GRF18U and BY4742 correspond to the parental strains transformed with the empty plasmids harboring in the productive strains the genes of the L-AA pathway). The control cells, as well as the cells expressing <i>ScALO1</i> and <i>AtLGDH</i> can not accumulate L-ascorbic acid starting from D-glucose, therefore measured values correspond to the endogenous erythro-ascorbic acid. The standard deviation bars correspond to the data obtained from independent clones, and from independent growth and antioxidant determinations. Please note the different scale of the ordinate axes in the two graphs.</p

MOESM1 of Assessing an effective feeding strategy to optimize crude glycerol utilization as sustainable carbon source for lipid accumulation in oleaginous yeasts

Additional file 1. Additional Figures and Tables; Figures S1–S6, Tables S1–S2. Additional information; optimization of the feeding strategy