Coxiella and Bartonella spp. in bats (Chiroptera) captured in the Brazilian Atlantic Forest biome

AUTHORS - Michelle Santos Ferreira1, Alexandro Guterres1, Tatiana Rozental1*, Roberto Leonan Morim Novaes2, Emmanuel Messias Vilar3, Renata Carvalho de Oliveira1, Jorlan Fernandes1, Danielle Forneas1, Adonai Alvino Junior1, Martha Lima Brandão4, José Luis Passos Cordeiro4, Martín Roberto Del Valle Alvarez5, Sergio Luiz Althoff6, Ricardo Moratelli4, Pedro Cordeiro-Estrela3, Rui Cerqueira da Silva7 and Elba Regina Sampaio de Lemos1 - AFFILIATIONS - 1Laboratório de Hantaviroses e Rickettsioses, Pavilhão Helio e Peggy Pereira, 1 Pavimento, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, RJ, Brazil. 2Universidade Federal do Rio de Janeiro, Av. Pedro Calmon, 550, Cidade Universitária, Rio de Janeiro, Rio de Janeiro, RJ, Brazil. 3Laboratório de Mamíferos, Departamento de Sistemática e Ecologia, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba, Campus I, Castelo Branco, João Pessoa, PB, Brazil. 4Fundação Oswaldo Cruz, Fiocruz Mata Atlântica, Estrada Rodrigues Caldas, 3400, Taquara, Rio de Janeiro, RJ, Brazil. 5Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Rodovia Ilhéus - Itabuna, Km. 16 Salobrinho, Ilheus, BA, Brazil. 6Departamento de Ciências Naturais, Laboratório de Biologia Animal, Fundação Universidade Regional de Blumenau, Ccen, Dcn. FURB - Fundação Universidade Regional de Blumenau Itoupava Seca, Blumenau, SC, Brazil. 7Laboratório de Vertebrados, Departamento de Ecologia, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Av. Pedro Calmon, 550, Cidade Universitária, Rio de Janeiro, RJ, Brazil.Submitted by Sandra Infurna ([email protected]) on 2019-02-01T14:37:58Z No. of bitstreams: 1 jorlan_fernandes_etal_IOC_2018.pdf: 987842 bytes, checksum: ac7a643e97226c541e8143added4d88b (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2019-02-01T14:59:25Z (GMT) No. of bitstreams: 1 jorlan_fernandes_etal_IOC_2018.pdf: 987842 bytes, checksum: ac7a643e97226c541e8143added4d88b (MD5)Made available in DSpace on 2019-02-01T14:59:25Z (GMT). No. of bitstreams: 1 jorlan_fernandes_etal_IOC_2018.pdf: 987842 bytes, checksum: ac7a643e97226c541e8143added4d88b (MD5) Previous issue date: 2018Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Fiocruz Mata Atlântica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Fiocruz Mata Atlântica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Fiocruz Mata Atlântica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Múltipl autoria - ver em NotasBackground: The role of bats as reservoirs of zoonotic agents, especially pathogenic bacteria such as Bartonella and Coxiella, has been discussed around the world. Recent studies have identified bats as potential hosts of species from the proteobacteria phylum. In Brazil, however, the role of bats in the natural cycle of these agents is poorly investigated and generally neglected. In order to analyze the participation of bats in the epidemiology of diseases caused by Bartonella, Coxiella, Rickettsia, Anaplasma and Ehrlichia, we conducted a descriptive epidemiological study in three biogeographic regions of the Brazilian Atlantic Forest. Results: Tissues of 119 bats captured in preserved areas in the states of Rio de Janeiro, Bahia and Santa Catarina from 2014 to 2015 were submitted to molecular analysis using specific primers. Bartonella spp. was detected in 22 spleen samples (18.5%, 95% CI: 11.9–26.6), whose phylogenetic analysis revealed the generation of at least two independent clusters, suggesting that these may be new unique genotypes of Bartonella species. In addition, four samples (3.4%, 95% CI: 0.9–8.3) were positive for the htpAB gene of C. burnetii [spleen (2), liver (1) and heart (1)]. Rickettsia spp., Anaplasma and Ehrlichia were not identified. This is the first study reporting C. burnetii and Bartonella spp. infections in bats from the Atlantic Forest biome. Conclusions: These findings shed light on potential host range for these bacteria, which are characterized as important zoonotic pathogens

Coxiella and Bartonella spp. in bats (Chiroptera) captured in the Brazilian Atlantic Forest biome

Abstract Background The role of bats as reservoirs of zoonotic agents, especially pathogenic bacteria such as Bartonella and Coxiella, has been discussed around the world. Recent studies have identified bats as potential hosts of species from the proteobacteria phylum. In Brazil, however, the role of bats in the natural cycle of these agents is poorly investigated and generally neglected. In order to analyze the participation of bats in the epidemiology of diseases caused by Bartonella, Coxiella, Rickettsia, Anaplasma and Ehrlichia, we conducted a descriptive epidemiological study in three biogeographic regions of the Brazilian Atlantic Forest. Results Tissues of 119 bats captured in preserved areas in the states of Rio de Janeiro, Bahia and Santa Catarina from 2014 to 2015 were submitted to molecular analysis using specific primers. Bartonella spp. was detected in 22 spleen samples (18.5%, 95% CI: 11.9–26.6), whose phylogenetic analysis revealed the generation of at least two independent clusters, suggesting that these may be new unique genotypes of Bartonella species. In addition, four samples (3.4%, 95% CI: 0.9–8.3) were positive for the htpAB gene of C. burnetii [spleen (2), liver (1) and heart (1)]. Rickettsia spp., Anaplasma and Ehrlichia were not identified. This is the first study reporting C. burnetii and Bartonella spp. infections in bats from the Atlantic Forest biome. Conclusions These findings shed light on potential host range for these bacteria, which are characterized as important zoonotic pathogens