Electroolfactogram (EOG) Recording in the Mouse Main Olfactory Epithelium

Olfactory sensory neurons in the main olfactory epithelium (MOE) are responsible for detecting odorants and EOG recording is a reliable approach to analyze the peripheral olfactory function. However, recently we revealed that rodent MOE can also detect the air pressure caused by airflow. The sensation of airflow pressure and odorants may function in synergy to facilitate odorant perception during sniffing. We have reported that the pressure-sensitive response in the MOE can also be assayed by EOG recording. Here we describe procedures for pressure-sensitive as well as odorant-stimulated EOG measurement in the mouse MOE. The major difference between the pressure-sensitive EOG response and the odorant-stimulated response was whether to use pure air puff or use an odorized air puff

Stimulation of electro-olfactogram responses in the main olfactory epithelia by airflow depends on the type 3 adenylyl cyclase

Cilia of olfactory sensory neurons are the primary sensory organelles for olfaction. The detection of odorants by the main olfactory epithelium (MOE) depends on coupling of odorant receptors to the type 3 adenylyl cyclase (AC3) in olfactory cilia. We monitored the effect of airflow on electro-olfactogram (EOG) responses and found that the MOE of mice can sense mechanical forces generated by airflow. The airflow-sensitive EOG response in the MOE was attenuated when cAMP was increased by odorants or by forskolin suggesting a common mechanism for airflow and odorant detection. In addition, the sensitivity to airflow was significantly impaired in the MOE from AC3−/− mice. We conclude that AC3 in the MOE is required for detecting the mechanical force of airflow, which in turn may regulate odorant perception during sniffing

Disruption of type 3 adenylyl cyclase expression in the hypothalamus leads to obesity

Evidence from human studies and transgenic mice lacking the type 3 adenylyl cyclase (AC3) indicates that AC3 plays a role in the regulation of body weight. It is unknown in which brain region AC3 exerts such an effect. We examined the role of AC3 in the hypothalamus for body weight control using a floxed AC3 mouse strain. Here, we report that AC3 flox/flox mice became obese after the administration of AAV-CRE-GFP into the hypothalamus. Both male and female AC3 floxed mice showed heavier body weight than AAV-GFP injected control mice. Furthermore, mice with selective ablation of AC3 expression in the ventromedial hypothalamus also showed increased body weight and food consumption. Our results indicated that AC3 in the hypothalamus regulates energy balance

OPTIMAL SPATIAL ALLOCATION OF WASTE MANAGEMENT PRACTICES TO REDUCE PHOSPHORUS POLLUTION IN A WATERSHED

Phosphorus pollution from excessive litter application and municipal discharges causes eutorphication of lakes in the Eucha-Spavinaw watershed in eastern Oklahoma and western Arkansas. Consequent algae blooms impair the taste of drinking water supply drawn from the watershed and reduce the recreational values of the lakes. The paper shows how GIS data based biophysical modeling can be used to derive spatially optimal, least-cost allocation of agricultural management practices to be combined with optimal wastewater treatment activity from the point source in order to achieve socially optimal phosphorus load in the watershed. The optimal level of phosphorus load is determined by equating marginal abatement with marginal damage cost. Transportation activities in the model allow for transportation of litter within and out of the watershed. Results show uniform regulation of litter application is excessively costly relative to measures that encourage adoption of management practices that equate marginal abatement costs across pollution sources. The results also show that change in the land use patterns in a long-run and using alum based litter additives in short-run are economically efficient management options.Environmental Economics and Policy,

Type 1 adenylyl cyclase is essential for maintenance of remote contextual fear memory

Although molecular mechanisms for hippocampus-dependent memory have been extensively studied, much less is known about signaling events important for remote memory. Here we report that mice lacking type 1 adenylyl cyclase (AC1) are able to establish and retrieve remote contextual memory but unable to sustain it as long as wild-type mice. Interestingly, mice overexpressing AC1 show superior remote contextual memory even though they exhibit normal hippocampus-dependent contextual memory. These data illustrate that calcium coupling to cAMP contributes to the stability of remote memory and identifies AC1 as a potential drug target site to improve long-term remote memory

Comparing Student Performance, Attitudes And Preferences In An Introduction To Business Course: Online, Hybrid And Traditional Delivery Methods Who Makes The A Grade?

The present study compares three Introduction to Business courses delivered using three different teaching formats; online, hybrid and traditional methods. Findings indicate that while the traditional course received higher ratings by students, hybrid students outperformed students in the online and traditional course (n = 56). Thirty-five percent of the students in the hybrid course earned an A compared to 23 percent of the traditional students, while only 7 percent of the students enrolled in the online course completed the course with an A grade. Student attitudes also indicate once a student experiences a hybrid model course, there is strong preference for this type of delivery method. Although 85 percent of the students enrolled in the hybrid course had never enrolled in a distance learning course, 73 percent selected the hybrid format as their preference of delivery method. Strong support exists indicating that hybrid courses that are well designed create an atmosphere that increase student learning. Moreover, once students have a good hybrid experience, they are more likely to prefer courses designed as hybrids

Overexpression of the type 1 adenylyl cyclase in the forebrain leads to deficits of behavioral inhibition

The type 1 adenylyl cyclase (AC1) is an activity-dependent, calcium-stimulated adenylyl cyclase expressed in the nervous system that is implicated in memory formation. We examined the locomotor activity, and impulsive and social behaviors of AC1+ mice, a transgenic mouse strain overexpressing AC1 in the forebrain. Here we report that AC1+ mice exhibit hyperactive behaviors and demonstrate increased impulsivity and reduced sociability. In contrast, AC1 and AC8 double knock-out mice are hypoactive, and exhibit increased sociability and reduced impulsivity. Interestingly, the hyperactivity of AC1+ mice can be corrected by valproate, a mood-stabilizing drug. These data indicate that increased expression of AC1 in the forebrain leads to deficits in behavioral inhibition

Generalized Geologic Map for Land-Use Planning: Knox County, Kentucky

This map is not intended to be used for selecting individual sites. Its purpose is to inform land-use planners, government officials, and the public in a general way about geologic bedrock conditions that affect the selection of sites for various purposes. The properties of thick soils may supercede those of the underlying bedrock and should be considered on a site-to-site basis. At any site, it is important to understand the characteristics of both the soils and the underlying rock

Generalized Geologic Map for Land-Use Planning: Laurel County, Kentucky

This map is not intended to be used for selecting individual sites. Its purpose is to inform land-use planners, government officials, and the public in a general way about geologic bedrock conditions that affect the selection of sites for various purposes. The properties of thick soils may supercede those of the underlying bedrock and should be considered on a site-to-site basis. At any site, it is important to understand the characteristics of both the soils and the underlying rock