Optimized Dictionaries: A Semi-Automated Workflow of Concept Identification in Text-Data

Identifying social science concepts and measuring their prevalence and framing in text data has been a key task of scientists ever since. Whereas debates about text classifications typically contrast different approaches with each other, we propose a workflow that generates optimized dictionaries that are based on the complementary use of expert dictionaries, machine learning, and topic modeling. We demonstrate our case by identifying the concept of "territorial politics" in leading newspapers vis-à-vis parliamentary speeches in Spain (1976-2018) and the UK (1900-2018). We show that our optimized dictionaries outperform singular text-identification techniques with F1-scores around 0.9 for unseen data, even if the unseen data comes from a different political domain (media vs. parliaments). Optimized dictionaries have increasing returns and should be developed as a common good for researchers overcoming costly particularism

Dispersal within a spatially structured population of lesser kestrels: The role of spatial isolation and conspecific attraction

1. Factors governing dispersal rates have seldom been examined in spatially structured populations of vertebrates. We gathered information during 8 years on transfer rates between subpopulations in a spatially structured, growing population of colonial lesser kestrels Falco naumanni, and analysed the contribution of several variables related to spatial isolation and characteristics of both subpopulation of origin and destination on probabilities of dispersal. 2. Lesser kestrels were highly philopatric to their subpopulations, but first-breeders dispersed more often than adults (26% vs. 4%, n = 1706) because adults were reluctant to move from familiar areas. Frequency of subpopulation change was higher in females than in males (first-recruiters: 30% vs. 22%, n = 987; adults 5% vs. 1%, n = 719), according to their different breeding roles. However, all populational factors had an equal effect on individuals of different sex and dispersal status. 3. Movement rates decreased with inter-subpopulation distance - indicating that travelling to distant subpopulations may impose costs in terms of breeding prospects -and with the number of subpopulations, which increased during our study period. 4. Conspecific attraction strongly influenced the probability of dispersal: it was relatively higher in largely populated subpopulations, and individuals of large subpopulations were reluctant to change to others. These results were neither influenced by the size and breeding density of the subpopulations nor by habitat quality in terms of food availability or risk of predation, as indicated by breeding success of kestrels at each subpopulation. The number of conspecifics could be used by the kestrels as a cue of patch quality in terms of settlement options, and large subpopulations could be more easily detected by prospecting birds. 5. Our study highlights the fact that several assumptions of theoretical metapopulation modelling are often not fulfilled in nature. Both theoretical models and management strategies on spatially structured populations or metapopulations should thus consider the number, population size, and spatial distribution of local populations, as well as their relationships with the dispersal ability of the species.Peer Reviewe

Mass Spectrometric Detection of Formaldehyde-Crosslinked PBMC Proteins in Cell-Free DNA Blood Collection Tubes

Streck tubes are commonly used to collect blood samples to preserve cell-free circulating DNA. They contain imidazolidinyl urea as a formaldehyde-releasing agent to stabilize cells. We investigated whether the released formaldehyde leads to crosslinking of intracellular proteins. Therefore, we employed a shotgun proteomics experiment on human peripheral blood mononuclear cells (PBMCs) that were isolated from blood collected in Streck tubes, EDTA tubes, EDTA tubes containing formaldehyde, or EDTA tubes containing allantoin. The identified crosslinks were validated in parallel reaction monitoring LC/MS experiments. In total, we identified and validated 45 formaldehyde crosslinks in PBMCs from Streck tubes, which were also found in PBMCs from formaldehyde-treated blood, but not in EDTA- or allantoin-treated samples. Most were derived from cytoskeletal proteins and histones, indicating the ability of Streck tubes to fix cells. In addition, we confirm a previous observation that formaldehyde crosslinking of proteins induces a +24 Da mass shift more frequently than a +12 Da shift. The crosslinking capacity of Streck tubes needs to be considered when selecting blood-collection tubes for mass-spectrometry-based proteomics or metabolomic experiments

Enhanced expression of thioredoxin-interacting-protein regulates oxidative DNA damage and aging

The "free radical theory of aging" suggests that reactive oxygen species (ROS) are responsible for age-related loss of cellular functions and, therefore, represent the main cause of aging. Redox regulation by thioredoxin-1 (TRX) plays a crucial role in responses to oxidative stress. We show that thioredoxin-interacting protein (TXNIP), a negative regulator of TRX, plays a major role in maintaining the redox status and, thereby, influences aging processes. This role of TXNIP is conserved from flies to humans. Age-dependent upregulation of TXNIP results in decreased stress resistance to oxidative challenge in primary human cells and in Drosophila. Experimental overexpression of TXNIP in Hies shortens lifespan due to elevated oxidative DNA damage, whereas downregulation of TXNIP enhances oxidative stress resistance and extends lifespan

Проект системы отопления супермаркета в г. Тайга Кемеровской области.

РЕФЕРАТ Дипломная работа 118 с., 17 рисунков, 29 источников, 33 таблицы, 3 графика, 8 л. графического материала. ПОДБОР ТОРГОВОГО ХОЛОДИЛЬНОГО ОБОРУДОВАНИЯ, СХЕМА И ПРОЦЕСС РАБОТЫ ХОЛОДИЛЬНОГО ОБОРУДОВАНИЯ РАСЧЕТ ТЕПЛОВЫХ ПОТЕРЬ ЗДАНИЯ, ВОЗДУШНОЕ ОТОПЛЕНИЕ, ВОДЯНОЕ ОТОПЛЕНИЕ, ГИДРАВЛИЧЕСКИЙ РАСЧЁТ, АЭРОДИНАМИЧЕСКИЙ РАСЧЕТ Объектом расчета является здание супермаркета, расположенное в городе Тайга. Цель работы – подбор основного оборудования и комплектующих для бесперебойной работы торгового холодильного оборудования, а также проект воздушной системы отопления для данного помещения с использованием теплосъёма с конденсаторов торгового холодильного оборудования, для создания комфортных условий, пребывающих в нем людей. В ходе проекта был рассмотрен вариант воздушной системы отопленияABSTRACT Thesis 118 p., 17 figures, 29 sources 33 table 3 graphics, 8 l graphic material. SELECTION OF COMMERCIAL REFRIGERATION EQUIPMENT, DESIGN, AND PROCESS REFRIGERATION EQUIPMENT HEAT LOSS CALCULATION OF THE BUILDING AIR HEATING, WATER HEATING, HYDRAULIC CALCULATION, AERODYNAMICS CALCULATION The object of the calculation is the building of a supermarket located in the town of Taiga. Purpose – selection of major equipment and components for the smooth operation of refrigeration equipment, as well as a draft air heating system for the premises with the use of heat removal from the condensers commercial refrigeration equipment, to create a comfortable environment, the people staying in it. The project was considered an air heating system as an alternative to the existing water heating system. Reviewed sections of the safety and sustainability of the project, automation and economy. Final work is executed in a text editor Microsoft Word 2007

METTL3-Mediated m6A Modification Controls Splicing Factor Abundance and Contributes to Aggressive CLL

RNA splicing dysregulation underlies the onset and progression of cancers. In chronic lymphocytic leukemia (CLL), spliceosome mutations leading to aberrant splicing occur in ∼20% of patients. However, the mechanism for splicing defects in spliceosome-unmutated CLL cases remains elusive. Through an integrative transcriptomic and proteomic analysis, we discover that proteins involved in RNA splicing are posttranscriptionally upregulated in CLL cells, resulting in splicing dysregulation. The abundance of splicing complexes is an independent risk factor for poor prognosis. Moreover, increased splicing factor expression is highly correlated with the abundance of METTL3, an RNA methyltransferase that deposits N6-methyladenosine (m6A) on mRNA. METTL3 is essential for cell growth in vitro and in vivo and controls splicing factor protein expression in a methyltransferase-dependent manner through m6A modification-mediated ribosome recycling and decoding. Our results uncover METTL3-mediated m6A modification as a novel regulatory axis in driving splicing dysregulation and contributing to aggressive CLL.SignificanceMETTL3 controls widespread splicing factor abundance via translational control of m6A-modified mRNA, contributes to RNA splicing dysregulation and disease progression in CLL, and serves as a potential therapeutic target in aggressive CLL. See related commentary by Janin and Esteller, p. 176. This article is highlighted in the In This Issue feature, p. 171