The genome evolution and domestication of tropical fruit mango

Background: Mango is one of the world’s most important tropical fruits. It belongs to the family Anacardiaceae, which includes several other economically important species, notably cashew, sumac and pistachio from other genera. Many species in this family produce family-specific urushiols and related phenols, which can induce contact dermatitis. Results: We generate a chromosome-scale genome assembly of mango, providing a reference genome for the Anacardiaceae family. Our results indicate the occurrence of a recent whole-genome duplication (WGD) event in mango. Duplicated genes preferentially retained include photosynthetic, photorespiration, and lipid metabolic genes that may have provided adaptive advantages to sharp historical decreases in atmospheric carbon dioxide and global temperatures. A notable example of an extended gene family is the chalcone synthase (CHS) family of genes, and particular genes in this family show universally higher expression in peels than in flesh, likely for the biosynthesis of urushiols and related phenols. Genome resequencing reveals two distinct groups of mango varieties, with commercial varieties clustered with India germplasms and demonstrating allelic admixture, and indigenous varieties from Southeast Asia in the second group. Landraces indigenous in China formed distinct clades, and some showed admixture in genomes. Conclusions: Analysis of chromosome-scale mango genome sequences reveals photosynthesis and lipid metabolism are preferentially retained after a recent WGD event, and expansion of CHS genes is likely associated with urushiol biosynthesis in mango. Genome resequencing clarifies two groups of mango varieties, discovers allelic admixture in commercial varieties, and shows distinct genetic background of landraces

The genome evolution and domestication of tropical fruit mango

Background: Mango is one of the world’s most important tropical fruits. It belongs to the family Anacardiaceae, which includes several other economically important species, notably cashew, sumac and pistachio from other genera. Many species in this family produce family-specific urushiols and related phenols, which can induce contact dermatitis. Results: We generate a chromosome-scale genome assembly of mango, providing a reference genome for the Anacardiaceae family. Our results indicate the occurrence of a recent whole-genome duplication (WGD) event in mango. Duplicated genes preferentially retained include photosynthetic, photorespiration, and lipid metabolic genes that may have provided adaptive advantages to sharp historical decreases in atmospheric carbon dioxide and global temperatures. A notable example of an extended gene family is the chalcone synthase (CHS) family of genes, and particular genes in this family show universally higher expression in peels than in flesh, likely for the biosynthesis of urushiols and related phenols. Genome resequencing reveals two distinct groups of mango varieties, with commercial varieties clustered with India germplasms and demonstrating allelic admixture, and indigenous varieties from Southeast Asia in the second group. Landraces indigenous in China formed distinct clades, and some showed admixture in genomes. Conclusions: Analysis of chromosome-scale mango genome sequences reveals photosynthesis and lipid metabolism are preferentially retained after a recent WGD event, and expansion of CHS genes is likely associated with urushiol biosynthesis in mango. Genome resequencing clarifies two groups of mango varieties, discovers allelic admixture in commercial varieties, and shows distinct genetic background of landraces

The Periodicity of Entire Functions with Finite Order

This paper is concerned with the periodicity of entire functions with finite growth order, and some sufficient conditions are given. Let f is a transcendental entire function with finite growth order, zero is a Picard exceptional value of f, and a given differential monomial Qf of f is periodic, then f is also periodic. We are also interested in finding the following: let f is a transcendental entire function with finite growth order, d is a Picard exceptional value of f and fzΔcnfz is a periodic function, then f is also a periodic function. These results extend Yang’s conjecture

Statistical Analysis of Relationship between Daytime Lidar-Derived Planetary Boundary Layer Height and Relevant Atmospheric Variables in the Semiarid Region in Northwest China

Accurate identification of key parameters for data assimilation is important in simulating the planetary boundary layer height (PBLH) and structure evolution in numerical weather prediction models. In this study, surface observational data and lidar-derived PBLH on 42 cloudless days from June 2007 to May 2008 are used to quantify the statistical relationships between surface parameters and the PBLH at a semiarid climate observational site in Northwest China. The results indicate that surface upward long wave radiation, surface temperature, and surface sensible heat fluxes show strong correlations with the PBLH with correlation coefficients at a range of 0.63–0.72. But these parameters show varying correlation response time to the different stages of PBL development. Furthermore, the air temperature shows the highest correlation with the PBLH near the surface and the correlation decreases with increasing height

High-Durability Concrete with Supplementary Cementitious Admixtures Used in Corrosive Environments

Durability of concrete is of great significance to prolong the service life of concrete structures in corrosive environments. Aiming at the economical and environment-friendly production of concrete by comprehensive utilization of the supplementary cementitious materials made of industrial byproducts, the resistances to chloride penetration, sulfate attack, and frost of high-performance concrete were studied in this paper. Fifteen concretes were designed at different water–binder ratio with the changes of contents of fly ash (FA), silica fume (SF), ground granulated blast-furnace slag (GGBS), and admixture of sulfate corrosion-resistance (AS). The compressive strength, the total electric flux of chloride penetrability, the sulfate resistance coefficient, and the indices of freezing and thawing were measured. Results indicate that, depending on the chemical composition, fineness, and pozzolanic activity, the supplementary cementitious admixtures had different effects on the compressive strength and the durability of concrete; despite having a higher fineness and pozzolanic activity, the GGBS gave out a negative effect on concrete due to a similar chemical composition with cement; the SF and FA presented beneficial effects on concrete whether they were used singly with GGBS or jointly with GGBS; the AS improved the compressive strength and the sulfate corrosion resistance of concrete. In general, the grade of durability was positively related to the compressive strength of concrete. Except for the concretes admixed only with GGBS or with GGBS and FA, others had super durability with the compressive strength varying from 70 MPa to 113 MPa. The concretes with water to binder ratio of 0.29 and total binders of 500 kg/m3 admixed with 7% FA + 8% SF + 8% GGBS or 7% FA + 8% SF + 8% GGBS + (10~12)% AS presented the highest grades of resistances specified in China codes to chloride penetration, sulfate corrosion, and frost, while the compressive strength was about 100 MPa

Identification and Characterization of Lipase Activity and Immunogenicity of LipL from <i>Mycobacterium tuberculosis</i>

<div><p>Lipids and lipid-metabolizing esterases/lipases are highly important for the mycobacterial life cycle and, possibly, for mycobacterial virulence. In this study, we expressed 10 members of the Lip family of <i>Mycobacterium tuberculosis</i>. Among the 10 proteins, LipL displayed a significantly high enzymatic activity for the hydrolysis of long-chain lipids. The optimal temperature for the lipase activity of LipL was demonstrated to be 37°C, and the optimal pH was 8.0. The lipase active center was not the conserved motif G-x-S-x-G, but rather the S-x-x-K and GGG motifs, and the key catalytic amino acid residues were identified as G50, S88, and K91, as demonstrated through site-directed mutagenesis experiments. A three-dimensional modeling structure of LipL was constructed, which showed that the GGG motif was located in the surface of a pocket structure. Furthermore, the subcellular localization of LipL was demonstrated to be on the mycobacterial surface by Western blot analysis. Our results revealed that the LipL protein could induce a strong humoral immune response in humans and activate a CD8⁺ T cell-mediated response in mice. Overall, our study identified and characterized a novel lipase denoted LipL from <i>M</i>. <i>tuberculosis</i>, and demonstrated that LipL functions as an immunogen that activates both humoral and cell-mediated responses.</p></div

Analysis of lipase activity.

<p>(A) The total lipase activities were assayed using cell lysate supernatants from the 10 recombinant <i>M</i>. <i>smegmatis</i> strains. The <i>p</i>-NP palmitate (C₁₆) was used as the substrate for the lipase activities analysis. The values represent the means ± standard deviations (SD) of three independent experiments. (B) Lipase activity of LipL towards <i>p</i>-NP esters with various chain lengths (C2, acetate; C4, butyrate; C6, caproate; C8, caprylate; C12, laurate; C14, myristate; C16, palmitate; and C18, stearate). The values represent the means ± SD of three independent experiments. (C) The effect of pH on lipase activity of LipL. (D) The effect of temperature on lipase activity of LipL.</p

Primers used for PCR of 10 Lip family genes from <i>M</i>. <i>tuberculosis</i>.

<p>The restriction sites integrated into the sequences are underlined.</p><p>Primers used for PCR of 10 Lip family genes from <i>M</i>. <i>tuberculosis</i>.</p

Primers used in this study.

<p>Primers used in this study.</p

The analysis of the lipase activities of the mutants.

<p>The six residue substitutions of site-directed mutagenesis were G49A, G50A, G51A, S88A, K91A, and S361A. (A) A schematic of the LipL protein sequence with predicted catalytic motif and the mutations. (B) The experiment was performed in the standard lipase activity assays. The values represent the means ± SD of three independent experiments.</p