A Pilot Survey for CIII] Emission in the Reionization Era: Gravitationally-Lensed z∼7−8\sim7-8 Galaxies in the Frontier Fields Cluster Abell 2744

We report results of a search for CIII] $\lambda \lambda$1907,1909 {\AA} emission using Keck's MOSFIRE spectrograph in a sample of 7 $z_{phot}\sim7-8$ candidates ($H\sim27$) lensed by the Hubble Frontier Field cluster Abell 2744. Earlier work has suggested the promise of using the CIII] doublet for redshift confirmation of galaxies in the reionization era given $Ly\alpha$ ($\lambda$1216 {\AA}) is likely attenuated by the neutral intergalactic medium. The primary challenge of this approach is the feasibility of locating CIII] emission without advanced knowledge of the spectroscopic redshift. With an integration time of 5 hours in the H-band, we reach a $5\sigma$ median flux limit (in between the skylines) of $1.5\times10^{-18}$ ergs cm$^{-2}$ sec$^{-1}$ but no convincing CIII] emission was found. We also incorporate preliminary measurements from two other CLASH/HFF clusters in which, similarly, no line was detected, but these were observed to lesser depth. Using the known distribution of OH emission and the photometric redshift likelihood distribution of each lensed candidate, we present statistical upper limits on the mean total CIII] rest-frame equivalent width for our $z\simeq7-8$ sample. For a signal/noise ratio of 5, we estimate the typical CIII] doublet rest-frame equivalent width is, with 95\% confidence, $<26\pm5$ {\AA}. Although consistent with the strength of earlier detections in brighter objects at $z\simeq6-7$, our study illustrates the necessity of studying more luminous or strongly-lensed examples prior to the launch of the James Webb Space Telescope.Comment: 7 pages, 4 figures, 1 table; accepted for publication in ApJ Letter

Forest stand conditions after Phytophthora ramorum management in northern California: post-treatment observations inform future responses

In 2006, several isolated Phytophthora ramorum infested locations were selected just outside an 800-ha infested area in southern Humboldt County, California, for silvicultural treatments that targeted the removal and/or reduction of tanoak (Notholithocarpus densiflorus) and California bay laurel (Umbellularia californica), the main tree hosts supporting sporulation of P. ramorum. The treatments included cutting of hosts, cutting with burning, and herbicide removal. Important differences emerged between the treatment responses in shrub cover, tanoak resprouting, fuel loads, and host tree regeneration. In 2010, another isolated infestation was verified in northern Humboldt County. This infestation was 80 km away from the core infested area in southern Humboldt. Stand-level P. ramorum disease dynamic models and forest growth simulations parameterized with data collected in this northern infestation suggested that California bay laurel and tanoak thinning treatments may slow tanoak mortality, foster a greater component of mature tanoak over the next 100 years, and contribute to greater amounts of live-tree carbon. Reviewing these long-running, landscape-scale P. ramorum management projects provides a platform to inform future adaptive management of P. ramorum. This paper summarizes two presentations from the Sixth Sudden Oak Death Science Symposium held in June 2016 in San Francisco, California

The use of healthcare datasets to investigate the impact of dose intensity in the adolescent and young adult cancer population.

Objectives • To investigate whether the dose intensity of chemotherapy received by Adolescent and Young Adult (AYA) cancer patients impacts on their survival. • To assess and compare the utility of existing healthcare data available at a regional, national and international level to answer this question. Approach A regional dataset has been created through linkage of the Yorkshire Specialist Register of Cancer in Children and Young People to electronic chemotherapy prescribing data from Leeds Teaching Hospitals NHS Trust, providing a detailed dataset.  The national dataset comprises of data from the National Cancer Registry and Analysis Service (NCRAS) linked to the Systemic Anti-Cancer Therapy (SACT) dataset which collects chemotherapy prescribing data from all hospitals in England. This dataset provides bigger patient numbers but data at a pseudonymised level.  The international dataset comprises of anonymised data from clinical trials. Results The data has being linked, cleaned and validated.  Survival analysis is being carried out using a causal inference framework. Conclusion This study will describe the value of existing healthcare care sets in the AYA cancer population.  Identification of areas in which the datasets are lacking will help inform data controllers regarding ways in which data collection can be optimised for this important patient group

Human β-Defensin 2 Expression in Oral Epithelium: Potential Therapeutic Targets in Oral Lichen Planus

Human β-defensin 2 (hBD-2) is a potent antimicrobial peptide that participates in defense against invading bacteria. We recently showed that bacterial components and histamine, through histamine H4 receptor (H4R), are involved in the pathogenesis of the potentially malignant lesion, oral lichen planus (OLP). However, the underlying mechanisms remain unknown. We, therefore, investigated the role of hBD2–histamine crosstalk signaling in promoting OLP pathology. Biopsies from OLP and oral tongue squamous cell carcinoma (OTSCC) patients, and healthy controls were used. Two OTSCC cell lines and normal human oral keratinocytes (HOKs) were used. HBD-2 and other targets were mapped by immunostaining and analyzed by ImageJ2 software. The highly sensitive droplet-digital PCR technology and qRT-PCR were utilized to study the clinically derived and in vitro samples, respectively. H4R was challenged with the specific agonist HST-10 and inverse agonist ST-1007. HBD-2 was highly induced in OLP lesions. In contrast, hBD2 expression was attenuated in OTSCC tissues, while very low levels of hBD-2 messenger RNA (mRNA) were observed in OTSCC cells. Together with tumor necrosis factor-α (TNF-α), histamine upregulated hBD-2 mRNA expression in HOKs. Activation of H4R seems to modulate the expression of epithelial hBD-2. These findings suggest the involvement of hBD-2 in the pathogenesis of OLP and may, thus, be harnessed for therapeutic interventions in OLP

Human β-Defensin 2 Expression in Oral Epithelium: Potential Therapeutic Targets in Oral Lichen Planus

Human β-defensin 2 (hBD-2) is a potent antimicrobial peptide that participates in defense against invading bacteria. We recently showed that bacterial components and histamine, through histamine H4 receptor (H4R), are involved in the pathogenesis of the potentially malignant lesion, oral lichen planus (OLP). However, the underlying mechanisms remain unknown. We, therefore, investigated the role of hBD2–histamine crosstalk signaling in promoting OLP pathology. Biopsies from OLP and oral tongue squamous cell carcinoma (OTSCC) patients, and healthy controls were used. Two OTSCC cell lines and normal human oral keratinocytes (HOKs) were used. HBD-2 and other targets were mapped by immunostaining and analyzed by ImageJ2 software. The highly sensitive droplet-digital PCR technology and qRT-PCR were utilized to study the clinically derived and in vitro samples, respectively. H4R was challenged with the specific agonist HST-10 and inverse agonist ST-1007. HBD-2 was highly induced in OLP lesions. In contrast, hBD2 expression was attenuated in OTSCC tissues, while very low levels of hBD-2 messenger RNA (mRNA) were observed in OTSCC cells. Together with tumor necrosis factor-α (TNF-α), histamine upregulated hBD-2 mRNA expression in HOKs. Activation of H4R seems to modulate the expression of epithelial hBD-2. These findings suggest the involvement of hBD-2 in the pathogenesis of OLP and may, thus, be harnessed for therapeutic interventions in OLP

Urethral metastasis from non-seminomatous germ cell tumor: a case report

<p>Abstract</p> <p>Introduction</p> <p>We present a case of nonseminomatous germ cell tumor of the testes with acute urinary retention secondary to urethral metastasis. This presentation, and similar cases of urethral metastasis from this tumor, have not been reported previously.</p> <p>Case presentation</p> <p>A 35-year-old Caucasian man presented to hospital with a history of acute urinary retention. On examination he was found to have right testicular enlargement with raised β-human chorionic gonadotrophin, serum α-fetoprotein and lactate dehydrogenase levels. He underwent radical left inguinal orchidectomy and histology confirmed a nonseminomatous germ cell tumor of the testes. Cystoscopy carried out due to urinary retention showed penile metastasis and the biopsy confirmed metastatic malignant undifferentiated teratoma. Staging computed tomography scan and magnetic resonance imaging of the pelvis showed pulmonary, pelvic nodal, ischial and penile metastasis. The diagnosis of the International Germ Cell Cancer Collaborative Group of poor prognosis metastatic nonseminomatous germ cell tumor was made, following which he received four cycles of bleomycin, etoposide and cisplatin chemotherapy with curative intent. He had a complete marker and an excellent radiological response. He is currently under follow up.</p> <p>Conclusion</p> <p>The unusual presentation of lymphovascular spread in this case of nonseminomatous germ cell tumor highlights the need to include routine pelvic imaging in the assessment and follow up of testicular cancer.</p