Use of Cross-Species Amplification Markers for Pollen-Medicated Gene Flow Determination in \u3cem\u3eTrifolium Polymorphum\u3c/em\u3e Poiret

The species Trifolium polymorphum Poiret is endemic to Uruguay and is widespread in native grasslands throughout the country. Preliminary observations suggested that the aerial flowers are chasmogamous (open at maturity for potential cross-pollination) while the basal flowers are cleistogamous. Several approaches have been practised to determine the reproductive system of forage legumes by the aid of co-dominant markers (Real et al., 2004; Dalla Rizza et al., 2004). The aim of this study is to explore cross-species amplification as a quick approach to obtain co-dominant markers to study the breeding system of T. polymorphum

Differential parameters between cytosolic 2-Cys peroxiredoxins, PRDX1 and PRDX2

Peroxiredoxins are thiol-dependent peroxidases that function in peroxide detoxification and H2O2 induced signaling. Among the six isoforms expressed in humans, PRDX1 and PRDX2 share 97% sequence similarity, 77% sequence identity including the active site, subcellular localization (cytosolic) but they hold different biological functions albeit associated with their peroxidase activity. Using recombinant human PRDX1 and PRDX2, the kinetics of oxidation and hyperoxidation with H2O2 and peroxynitrite were followed by intrinsic fluorescence. At pH 7.4, the peroxidatic cysteine of both isoforms reacts nearly tenfold faster with H2O2 than with peroxynitrite, and both reactions are orders of magnitude faster than with most protein thiols. For both isoforms, the sulfenic acids formed are in turn oxidized by H2O2 with rate constants of ca 2 × 103 M−1 s−1 and by peroxynitrous acid significantly faster. As previously observed, a crucial difference between PRDX1 and PRDX2 is on the resolution step of the catalytic cycle, the rate of disulfide formation (11 s−1 for PRDX1, 0.2 s−1 for PRDX2, independent of the oxidant) which correlates with their different sensitivity to hyperoxidation. This kinetic pause opens different pathways on redox signaling for these isoforms. The longer lifetime of PRDX2 sulfenic acid allows it to react with other protein thiols to translate the signal via an intermediate mixed disulfide (involving its peroxidatic cysteine), whereas PRDX1 continues the cycle forming disulfide involving its resolving cysteine to function as a redox relay. In addition, the presence of C83 on PRDX1 imparts a difference on peroxidase activity upon peroxynitrite exposure that needs further study.Fil: Dalla Rizza, Joaquín. Universidad de la Republica; UruguayFil: Randall, Lía M.. Universidad de la Republica; UruguayFil: Santos, Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Ferrer Sueta, Gerardo. Universidad de la Republica; UruguayFil: Denicola, Ana. Universidad de la República; Urugua

Use of Genuine Sources of Ergot Resistance in Species of the \u3ci\u3edilatata\u3c/i\u3e Group of \u3ci\u3ePaspalum\u3c/i\u3e

Paspalum dilatatum Poir. (dallisgrass) is an excellent C4 forage for summer production, highly productive with a wide distribution within warm-temperate regions. P. dilatatum is native to South America with special relevance for milk and meat production since its forage quality is superior to that of other C4 forage grasses and it shows tolerance to frost and water stress. In situations of temperate and warm temperate climate, the presence of these species plays a key role in the ecological stability of the system due to the complementarity of the growth cycles between winter and summer grasses. Claviceps paspali (ergot) is a fungus that mainly parasitizes Paspalum spp., generating a structure called sclerotia, in which indole-diterpenoid alkaloids are isolated. Despite the excellent forage characteristics of Paspalum species, there is a need for the generation of varieties able to overcome two major limitations that were identified early on in this species, such as ergot susceptibility and seed production. With this objective, selected genotypes were crossed between apomictic and sexual species of different ploidy using P. malacophyllum as a source of immunity. Immunity to ergot has been evaluated in the field for two years in different representative regions of Uruguay and the accessions that did not get sick were selected as pollen donors. Crosses made with P. flavescens showed a germination percentage ranging between 1.7 and 7.09, while in P. dilatatum var. Chirú the range was reduced between 0.99 and 1.25 according to the employed parents. The hybrid nature of the progeny is being verified by microsatellites and functional markers associated with immunity and DNA content estimated by flow cytometry. This work aims to generate the basis to transfer immunity from P. malacophyllum in selected genotypes of Paspalum species predominant in Pampa biome and to improve seed production

Dipeptidyl Peptidase-4 Inhibition by Vildagliptin and the Effect on Insulin Secretion and Action in Response to Meal Ingestion in Type 2 Diabetes

OBJECTIVE—The purpose of this study was to determine the mechanism by which dipeptidyl peptidase-4 inhibitors lower postprandial glucose concentrations

Codes of Commitment to Crime and Resistance: Determining Social and Cultural Factors over the Behaviors of Italian Mafia Women

This article categorizes thirty-three women in four main Italian Mafia groups and explores social and cultural behaviors of these women. This study introduces the feminist theory of belief and action. The theoretical inquiry investigates the sometimes conflicting behaviors of women when they are subject to systematic oppression. I argue that there is a cultural polarization among the categorized sub-groups. Conservative radicals give their support to the Mafia while defectors and rebels resist the Mafia. After testing the theory, I assert that emancipation of women depends on the strength of their beliefs to perform actions against the Mafiosi culture

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field