Transcription factor induction of vascular blood stem cell niches in vivo

The hematopoietic niche is a supportive microenvironment composed of distinct cell types, including specialized vascular endothelial cells that directly interact with hematopoietic stem and progenitor cells (HSPCs). The molecular factors that specify niche endothelial cells and orchestrate HSPC homeostasis remain largely unknown. Using multi-dimensional gene expression and chromatin accessibility analyses in zebrafish, we define a conserved gene expression signature and cis-regulatory landscape that are unique to sinusoidal endothelial cells in the HSPC niche. Using enhancer mutagenesis and transcription factor overexpression, we elucidate a transcriptional code that involves members of the Ets, Sox, and nuclear hormone receptor families and is sufficient to induce ectopic niche endothelial cells that associate with mesenchymal stromal cells and support the recruitment, maintenance, and division of HSPCs in vivo. These studies set forth an approach for generating synthetic HSPC niches, in vitro or in vivo, and for effective therapies to modulate the endogenous niche

McDonnell XH-20 Little Henry

A McDonnell XH-20 named Little Henry at Wright Patterson Air Force Base.https://corescholar.libraries.wright.edu/special_ms458_photographs_air_force_history/1009/thumbnail.jp

Evidence-based medicinal plants for modern chronic diseases

[No abstract available

Computed and experimental interactions between eddy structure and dispersed particles in developing free shear layers

We are investigating the interactive process between turbulent flow and dispersed phase particles. We are focusing on the mechanisms that appear to result in a reduction of local turbulent intensity and a corresponding reduction in wall heat transfer and subsequent wall erosion in turbulent solid propellant combustion flow. We apply computational simulations and physical experiments specialized to a developing free shear layer over a rearward facing step and over a parallel splitter plate. The flow configuration evolves in a two-dimensional, steady, combustion and non-combustion turbulent free shear mixing region, with and without particle additives. The computational simulations combine three basic components: gas phase Navier-Stokes solutions, Lagrange particle field solutions and a Monte Carlo technique for the random encounters, forces and accelerations between the two fields. We concentrate here on relatively large sized additive particles (of the order of tens of microns to 100 microns mean diameter). We examine their apparent influence in breaking up the larger, energy bearing eddy structures into smaller structures which are more readily dissipated