1,374 research outputs found
Silica passivated conjugated polymer nanoparticles for biological imaging applications
Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However, as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new fluorescent probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, bright, photo-stable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell. By incubating the CN-PPV silica shelled cross-linked (SSCL) nanoparticles in mammalian (HeLa) cells; we were able to show that cellular uptake occurred. Uptake was also shown by incubating the nanoparticles in RWPE-1, WPE1-NB26 and WPE1- NA22 prostate cancer cell lines. Finally, HEK cells were used to show the particles had limited cytotoxicity
Does Gender Discrimination Impact Regular Mammography Screening? Findings from the Race Differences in Screening Mammography Study
Objective: To determine if gender discrimination, conceptualized as a negative life stressor, is a deterrent to adherence to mammography screening guidelines.
Methods: African American and white women (1451) aged 40–79 years who obtained an index screening mammogram at one of five urban hospitals in Connecticut between October 1996 and January 1998 were enrolled in this study. This logistic regression analysis includes the 1229 women who completed telephone interviews at baseline and follow-up (average 29.4 months later) and for whom the study outcome, nonadherence to age-specific mammography screening guidelines, was determined. Gender discrimination was measured as lifetime experience in seven possible situations.
Results: Gender discrimination, reported by nearly 38% of the study population, was significantly associated with non-adherence to mammography guidelines in women with annual family incomes of $50,000 or greater (or 1.99, 95% CI 1.33, 2.98) and did not differ across racial/ethnic groups.
Conclusions: Our findings suggest that gender discrimination can adversely influence regular mammography screening in some women. With nearly half of women nonadherent to screening mammography guidelines in this study and with decreasing mammography rates nationwide, it is important to address the complexity of nonadherence across subgroups of women. Life stressors, such as experiences of gender discrimination, may have considerable consequences, potentially influencing health prevention prioritization in women
Vertex-Coloring with Star-Defects
Defective coloring is a variant of traditional vertex-coloring, according to
which adjacent vertices are allowed to have the same color, as long as the
monochromatic components induced by the corresponding edges have a certain
structure. Due to its important applications, as for example in the
bipartisation of graphs, this type of coloring has been extensively studied,
mainly with respect to the size, degree, and acyclicity of the monochromatic
components.
In this paper we focus on defective colorings in which the monochromatic
components are acyclic and have small diameter, namely, they form stars. For
outerplanar graphs, we give a linear-time algorithm to decide if such a
defective coloring exists with two colors and, in the positive case, to
construct one. Also, we prove that an outerpath (i.e., an outerplanar graph
whose weak-dual is a path) always admits such a two-coloring. Finally, we
present NP-completeness results for non-planar and planar graphs of bounded
degree for the cases of two and three colors
Survey Simulations of a New Near-Earth Asteroid Detection System
We have carried out simulations to predict the performance of a new
space-based telescopic survey operating at thermal infrared wavelengths that
seeks to discover and characterize a large fraction of the potentially
hazardous near-Earth asteroid (NEA) population. Two potential architectures for
the survey were considered: one located at the Earth-Sun L1 Lagrange point, and
one in a Venus-trailing orbit. A sample cadence was formulated and tested,
allowing for the self-follow-up necessary for objects discovered in the daytime
sky on Earth. Synthetic populations of NEAs with sizes >=140 m in effective
spherical diameter were simulated using recent determinations of their physical
and orbital properties. Estimates of the instrumental sensitivity, integration
times, and slew speeds were included for both architectures assuming the
properties of new large-format 10 um detector arrays capable of operating at
~35 K. Our simulation included the creation of a preliminary version of a
moving object processing pipeline suitable for operating on the trial cadence.
We tested this pipeline on a simulated sky populated with astrophysical sources
such as stars and galaxies extrapolated from Spitzer and WISE data, the catalog
of known minor planets (including Main Belt asteroids, comets, Jovian Trojans,
etc.), and the synthetic NEA model. Trial orbits were computed for simulated
position-time pairs extracted from the synthetic surveys to verify that the
tested cadence would result in orbits suitable for recovering objects at a
later time. Our results indicate that the Earth-Sun L1 and Venus-trailing
surveys achieve similar levels of integral completeness for potentially
hazardous asteroids larger than 140 m; placing the telescope in an interior
orbit does not yield an improvement in discovery rates. This work serves as a
necessary first step for the detailed planning of a next-generation NEA survey.Comment: AJ accepted; corrected typ
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The Experience of Discrimination and Black-White Health Disparities in Medical Care
The current study of Black patients focuses on how discrimination contributes to racial disparities in health. The authors used a longitudinal methodology to study how perceived past discrimination affects reactions to medical interactions and adherence to physician recommendations. In addition, they explored whether these reactions and/or adherence mediate the relationship between discrimination and patients' health. The participants in this study were 156 Black patients of low socioeconomic status at a primary care clinic. Patients completed questionnaires on their current health, past adherence, and perceived past discrimination. Then, they saw a physician and rated their reactions to the visit. Four and 16 weeks later they reported on their adherence to physician recommendations and overall health. Perceived discrimination was significantly and negatively associated with patient health, reactions to the physician, and adherence. Path analyses indicated that adherence mediated the relationship between discrimination and patient health, but patient reactions to the interaction did not
On Coloring Resilient Graphs
We introduce a new notion of resilience for constraint satisfaction problems,
with the goal of more precisely determining the boundary between NP-hardness
and the existence of efficient algorithms for resilient instances. In
particular, we study -resiliently -colorable graphs, which are those
-colorable graphs that remain -colorable even after the addition of any
new edges. We prove lower bounds on the NP-hardness of coloring resiliently
colorable graphs, and provide an algorithm that colors sufficiently resilient
graphs. We also analyze the corresponding notion of resilience for -SAT.
This notion of resilience suggests an array of open questions for graph
coloring and other combinatorial problems.Comment: Appearing in MFCS 201
Bright conjugated polymer nanoparticles containing a biodegradable shell produced at high yields and with tuneable optical properties by a scalable microfluidic device
This study compares the performance of a microfluidic technique and a conventional bulk method to manufacture conjugated polymer nanoparticles (CPNs) embedded within a biodegradable poly(ethylene glycol) methyl ether-block-poly(lactide-co-glycolide) (PEG5K–PLGA55K) matrix. The influence of PEG5K–PLGA55K and conjugated polymers cyano-substituted poly(p-phenylene vinylene) (CN-PPV) and poly(9,9-dioctylfluorene-2,1,3-benzothiadiazole) (F8BT) on the physicochemical properties of the CPNs was also evaluated. Both techniques enabled CPN production with high end product yields (?70–95%). However, while the bulk technique (solvent displacement) under optimal conditions generated small nanoparticles (∼70–100 nm) with similar optical properties (quantum yields ∼35%), the microfluidic approach produced larger CPNs (140–260 nm) with significantly superior quantum yields (49–55%) and tailored emission spectra. CPNs containing CN-PPV showed smaller size distributions and tuneable emission spectra compared to F8BT systems prepared under the same conditions. The presence of PEG5K–PLGA55K did not affect the size or optical properties of the CPNs and provided a neutral net electric charge as is often required for biomedical applications. The microfluidics flow-based device was successfully used for the continuous preparation of CPNs over a 24 hour period. On the basis of the results presented here, it can be concluded that the microfluidic device used in this study can be used to optimize the production of bright CPNs with tailored properties with good reproducibility
Facilitating the elicitation of beliefs for use in Bayesian Belief modelling
Expert opinion is increasingly being used to inform Bayesian Belief Networks, in particular to define the conditional dependencies modelled by the graphical structure. The elicitation of such expert opinion remains a major challenge due to both the quantity of information required and the ability of experts to quantify subjective beliefs effectively. In this work, we introduce a method designed to initialise conditional probability tables based on a small number of simple questions that capture the overall shape of a conditional probability distribution before enabling the expert to refine their results in an efficient way. These methods have been incorporated into a software Application for Conditional probability Elicitation (ACE), freely available at https://github.com/KirstyLHassall/ACE Hassall (2019
Self-paced reaching after stroke: a quantitative assessment of longitudinal and directional sensitivity using the H-Man planar robot for upper limb neurorehabilitation
Technology aided measures offer a sensitive, accurate and time-efficient approach for the assessment of sensorimotor function after neurological insult compared to standard clinical assessments. This study investigated the sensitivity of robotic measures to capture differences in planar reaching movements as a function of neurological status (stroke, healthy), direction (front, ipsilateral, contralateral), movement segment (outbound, inbound), and time (baseline, post-training, 2-week follow-up) using a planar, two-degrees of freedom, robotic-manipulator (H-Man). Twelve chronic stroke (age: 55 ± 10.0 years, 5 female, 7 male, time since stroke: 11.2 ± 6.0 months) and nine aged-matched healthy participants (age: 53 ± 4.3 years, 5 female, 4 male) participated in this study. Both healthy and stroke participants performed planar reaching movements in contralateral, ipsilateral and front directions with the H-Man, and the robotic measures, spectral arc length (SAL), normalized time to peak velocities (TpeakN), and root-mean square error (RMSE) were evaluated. Healthy participants went through a one-off session of assessment to investigate the baseline. Stroke participants completed a 2-week intensive robotic training plus standard arm therapy (8 × 90 min sessions). Motor function for stroke participants was evaluated prior to training (baseline, week-0), immediately following training (post-training, week-2), and 2-weeks after training (follow-up, week-4) using robotic assessment and the clinical measures Fugl-Meyer Assessment (FMA), Activity-Research-Arm Test (ARAT), and grip-strength. Robotic assessments were able to capture differences due to neurological status, movement direction, and movement segment. Movements performed by stroke participants were less-smooth, featured longer TpeakN, and larger RMSE values, compared to healthy controls. Significant movement direction differences were observed, with improved reaching performance for the front, compared to ipsilateral and contralateral movement directions. There were group differences depending on movement segment. Outbound reaching movements were smoother and featured longer TpeakN values than inbound movements for control participants, whereas SAL, TpeakN, and RMSE values were similar regardless of movement segment for stroke patients. Significant change in performance was observed between initial and post-assessments using H-Man in stroke participants, compared to conventional scales which showed no significant difference. Results of the study indicate the potential of H-Man as a sensitive tool for tracking changes in performance compared to ordinal scales (i.e., FM, ARAT)
WISE/NEOWISE Preliminary Analysis and Highlights of the 67P/Churyumov-Gerasimenko Near Nucleus Environs
On January 18-19 and June 28-29 of 2010, the Wide-field Infrared Survey
Explorer (WISE) spacecraft imaged the Rosetta mission target, comet
67P/Churyumov-Gerasimenko. We present a preliminary analysis of the images,
which provide a characterization of the dust environment at heliocentric
distances similar to those planned for the initial spacecraft encounter, but on
the outbound leg of its orbit rather than the inbound. Broad-band photometry
yields low levels of CO2 production at a comet heliocentric distance of 3.32 AU
and no detectable production at 4.18 AU. We find that at these heliocentric
distances, large dust grains with mean grain diameters on the order of a
millimeter or greater dominate the coma and evolve to populate the tail. This
is further supported by broad-band photometry centered on the nucleus, which
yield an estimated differential dust particle size distribution with a power
law relation that is considerably shallower than average. We set a 3-sigma
upper limit constraint on the albedo of the large-grain dust at <= 0.12. Our
best estimate of the nucleus radius (1.82 +/- 0.20 km) and albedo (0.04 +/-
0.01) are in agreement with measurements previously reported in the literature
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