Comparison of platelet rich plasma versus fibrin glue on colonic anastomoses in rats(1)

WOS: 000432449900005PubMed ID: 29768536Purpose:To compare platelet rich plasma (PRP) and fibrin glue aboutthe effect of anastomotic healing. Methods: Thirty six Wistar-Albino male rats diveded into 3 groups accord ingto control(Group1), PRP (Group 2) and fibrin glue(Tisseel VH) (Group 3). The colon was transected with scissor and subsequently an end to end anastomosis was performed using continuous one layer 6/0 vicryl sutures. Postoperative 7th day effect of anastomotic healing measuring with tissue hydroxyproline(TH) level and anastomotic bursting pressure(ABP); moreover comparison of cytokine (IL-6 and IL-10) and procalcitonin levels on 1st,3rd and 7th days. Results: There was no statistically significant difference of the ABP and hydroxyproline levels between PRP and fibrin glue on the 7th day. There was no statistically significant difference between levels of proinflammatory cytokine (IL-6) (P=0.41), anti-inflammatory cytokine (IL-10) (P=0.35), and procalcitonin levels (P=0.63) on 1, 3 and 7 days. Conclusion: Fibrin glue and platelet rich plasma are shown to be effective in healing intestinal anastomoses without superior to each other

Changes In Various Hormone Levels In The Rabbit Traumatic Facial Nerve Injury Model

WOS: 000493730200005Objectives: We aimed to look into potential associations between specific biomarkers and trauma to Cranial Nerve VII (CNVII) in a rabbit model, focusing on whether endocrine studies have potential as biomarkers in this context. Methods: 30 adult New Zealand rabbits with intact facial muscles were used for the research. Each animal underwent identical surgery by the same surgeon. The facial nerve divisions were exposed by incising below the level of the mandible. After dissection of the skin and subcutaneous layers, the buccal division of CNVII was located with the nerve stimulator and microscopic dissection and a section of nerve 10mm long was excised in each case from the buccal branch of CNVII. Blood samples were drawn 8 weeks and 12 weeks after nerve injury had been surgically induced. The samples from day 1, week 4 and week 8 were tested for the following levels: Testosterone, oestrogen, progesterone, free T3 and T4, Cancer antigen 19-9 (Ca19-9), folate, TPSA, FPSA, FSH, LH, CA15.3, CAE, AFP and prolactin. Results: The levels of free T3 and T4 as well as testosterone, were down at 4th week, but at 8 weeks each had increased. Ca19-9 levels were also above the baseline. At 4 weeks, whilst oestrogen had markedly risen, progesterone had fallen. The statistical significance of the change in levels of free T3 and T4, testosterone, oestrogen, progesterone and FPSA was evaluated. For the group of animals with induced paralysis, the association between the lesion and testosterone, oestrogen, progesterone, free T3 and T4, Ca19-9, and folate levels were strong and at the level of statistical significance. Conclusion: There were statistically significant alterations in the serum levels of free T3 and T4, testosterone, oestrogen, progesterone and FPSA at the 4 and 8 week intervals post surgically-induced CNVII injury. It is likely that rabbit pathophysiology resembles human pathophysiology in nervous injury, hence these six biomarkers may be of value in managing trauma or idiopathic degeneration of CNVII in humans. The authors hope this study will pave the way for future research in this area

Assessment of The Antimicrobial Effect of Manuka Honey in The Implant- Related Spinal Infections in Rats

WOS: 000328090600009Aim: Implant-related infections in spinal surgical procedures are still an important problem despite all the precautions. Various methods and medicinal products have been applied in order to prevent implant-related infections. "Manuka honey" is a type of honey which has been approved by FDA as a medicinal product. "Manuka honey" has bactericidal and bacteriostatic effects on "methicillin resistant Staphylococcus aureus" which is the most frequently isolated bacteria. The purpose of this study is to evaluate whether as an inexpensive and natural product, honey would prevent infection growth on rats in the implant related infection model. Method: Rats were divided into 3 groups, each of which containing 8 rats: namely, the control, infection and treatment groups. In the 1st group, there was no bacterial proliferation. Planted standard MRSA strain has been detected on rats belonging to the groups 2 and 3. On the vertebral column and implants, bacterial growth was greater in the 2nd group than the 3rd group. Also more bacterial colony growth has been detected in 2nd group as compared to 3rd group. While this difference was deemed significant in implant, the same did not apply to vertebral column. Result: Manuka honey could not completely eradicate the MRSA infection; however, it did decrease the intensity of the infection. If manuka honey, through constituting a different model, could be used in infections regularly, more objective and promising results would be revealed. Moreover, we consider that experimenting "manuka honey" on different bacteria including other Staph. aureus strains would be appropriate through constituting different models

Impacts of beta-estradiol on intestinal injury in newborn rats

WOS: 000373516000011PubMed ID: 26305144Objective: Necrotizing enterocolitis has been investigated and debated extensively in recent years; however, there is still no effective treatment. The aim of this study was thus to examine the effects of -estradiol on intestinal injury in rats.Methods: Twenty-four newborn female rat pups were divided into three groups. In group 1 (sham), hypoxia-re-oxygenation was not performed. In group 2 (saline), the rats were injected with saline after hypoxia-re-oxygenation, and the process was repeated for 5d. In group 3 (-estradiol treatment), the rats were subjected to hypoxia-re-oxygenation and then given -estradiol intraperitoneally once a day for 5d. After these procedures, the terminal ileum was removed for analysis.Results: Statistically significant differences in histological grades were found between groups 1 and 2 (p=0.000), groups 1 and 3 (p=0.028), and groups 2 and 3 (p=0.021). There were also differences in TNF- and IL-6 levels between groups 2 and 3 (p=0.000 and p=0.038, respectively) and between groups 1 and 2 (p=0.000 and p=0.000); there was no difference between groups 1 and 3 (p=0.574 and p=0.195, respectively). Electron microscopy examination revealed a decrease in lipid droplets at the apical cytoplasm of the columnar cells in group 2; in group 3, the absorption of the lipids as lipid droplets was similar to that of group 1.Conclusion: In this study, -estradiol was found to decrease the intensity of intestinal injury significantly by inhibiting TNF- and IL-6

Therapeutic Potential of Pterostilbene and Resveratrol on Biomechanic, Biochemical, and Histological Parameters in Streptozotocin-Induced Diabetic Rats

Aims. The aim of this study was to investigate the effects of pterostilbene (PTS) (trans-3,5-dimethoxy-4′-hydroxystilbene) and resveratrol (RSV) (trans-3,5,4′ trihydroxystilbene) applied at different doses for the treatment of streptozotocin- (STZ-) induced diabetic rats. Materials and Methods. At the end of the 5-week experimental period, the right gastrocnemius muscles of the rats were examined biomechanically, while the left ones were examined histologically. In addition, blood glucose, serum insulin, and malondialdehyde (MDA) levels were analyzed in blood samples taken from the rats. Results. The skeletal muscle isometric contraction forces, which showed a decrease with diabetes, were observed to increase with antioxidant applications. Blood glucose, serum insulin, and MDA levels in diabetic rats approached normal levels after applying PTS. When the electron microscopic images of the rat skeletal muscle were examined, those in the combination treatment group were observed to show a better enhancement in the skeletal muscle morphological structure compared to the other diabetic and treatment groups. Conclusion. According to the findings, we suggest that these antioxidant treatments might have good therapeutic nutraceutical potential for some muscle diseases that coexist with diabetes. These treatments should be comprehensively investigated in the future