Genetic analysis of water loss of excised leaves associated with drought tolerance in wheat

BACKGROUND: . Wheat is widely affected by drought. Low excised-leaf water loss (ELWL) has frequently been associated with improved grain yield under drought. This study dissected the genetic control of ELWL in wheat, associated physiological, morphological and anatomical leaf traits, and compared these with yield QTLs. Methods. Ninety-four hexaploid wheat (Triticum aestivum L.) doubled haploids, mapped with over 700 markers, were tested for three years for ELWL from detached leaf 4 of glasshouse-grown plants. In one experiment, stomata per unit area and leaf thickness parameters from leaf cross-sections were measured. QTLs were identified using QTLCartographer. Results. ELWL was significantly negatively correlated with leaf length, width, area and thickness. Major QTLs for ELWL during 0-3 h and 3-6 h were coincident across trials on 3A, 3B, 4B, 5B, 5D, 6B, 7A, 7B, 7D and frequently coincident (inversely) with leaf size QTLs. Yield in other trials was sometimes associated with ELWL and leaf size phenotypically and genotypically, but more frequently under non-droughted than droughted conditions. QTL coincidence showed only ELWL to be associated with drought/control yield ratio. Discussion. Our results demonstrated that measures of ELWL and leaf size were equally effective predictors of yield, and both were more useful for selecting under favourable than stressed conditions

Effect of Morphine on the Number of Adenohypophysis Acidophil Cells in Male Rat

Abstract: Introduction: The influence of morphine on pituitary secretion through µmu receptors has been nearly known. It is also reported that morphine has an effect on cell proliferation. In the present study we investigated the effect of morphine on the proliferation of acidophil cells of adenohypophysis in male rat. Method: This study has been carried out on 14 adult male Wistar rats divided into two groups of morphine dependent and control. The animals in morphine-dependent group were addicted through consumption of morphine for 21 days. After controlling withdrawal syndrome signs, serum prolactin level was determined by Elisa method. In the next step after anesthetizing animals and performing cardiac perfusion, the hypophysis was removed and fixed at 10% formalin. After processing, staining was done by routine immuno-histochemistry method and the number of mammotropes and somatotropes in morphine-dependent and control groups were compared. Results: Mean prolactin production in dependent group as compared with control group showed significant increase. There was a significant increase in the ratio of mammotropes to acidophil cells and a significant decrease in the ratio of somototrops to acidophil cells in morphine-dependent group compared to the control group (P<0.05). Conclusion: Morphine dependency may lead to increase in the percentage of prolactin secreting cells and serum prolactin level and decrease of growth hormone secreting cells. Keywords: Morphine dependence, Adenohypophysis, Acidophil cells, Ra

Frequency, Severity, Rate, and Causes of Moral Distress among Nursing Students: A Cross-Sectional Study

Background. One of the most important psychosocial issues related to the nursing profession is moral distress (MD), which has devastating effects on student performance as well as patient care. The current study was conducted to determine the frequency, severity, rate, and causes of MD in nursing students. Methods. During this cross-sectional study, 86 samples were last year undergraduate nursing students of Kermanshah University of Medical Sciences, who were entered into the study by the census method. Data gathering instruments were personal information form, Moral Distress Scale-revised (MDS-r), and Moral Distress Thermometer (MDT). Results. The mean frequency, severity, and rate of MD, according to the MDS-r questionnaire, were 1.3 ± 0.7 out of 4, 1.8 ± 0.8 out of 4, and 3.3 ± 2.3 out of 16, respectively. The average MDT score was 2.6 ± 2.5 out of 10. The most common causes of MD were working in unsafe conditions (6.8 ± 6.0), observing impaired patients health servicing (5.4 ± 5.6), and the low quality of patient care (5.1 ± 4.6). There was a statistically significant relationship between the gender, work experience, and participation in the professional ethics workshop variables and mean MDS-total and MDT. Conclusion. The rate and mean severity of MD were reported at a moderate level. Also, the MDT average was reported at a mild to an uncomfortable level, and the overall MD was equal to moderate. Therefore, student administrators should take steps to eliminate or reduce the causes of MD. In this regard, the need for teaching preventive methods against MD to nursing students before their clinical courses seems crucial. Also, students with MD must be referred to the psychiatric/psychologist