Measurement and modelling of evapotranspiration in three fynbos vegetation types

Many studies have investigated the water relations of indigenous plants in the fynbos shrublands of the Cape, South Africa. These have mainly focused on understanding the mechanisms by which individual plant species respond to droughts, the frequency and severity of which is expected to increase due to climate change. However, comparatively little information exists on the dynamics of water use by indigenous plants in the region, and, in particular, how water use varies seasonally and between sites. In this study we determined water use by 3 fynbos vegetation types growing at 4 different sites, namely: (i) lowland Atlantis Sand Plain fynbos growing on deep sandy soils, (ii) Kogelberg Sandstone fynbos growing in a riparian zone on deep alluvial soils, (iii) dryland Kogelberg Sandstone fynbos growing on shallow sandy soils at a montane site, and (iv) alluvial Swartland fynbos growing in clayey soils. Evapotranspiration (ET) was quantified at each site during specific periods using a boundary layer scintillometer and energy balance system. A simple dual source model in which the stand ET was calculated as the algebraic sum of outputs from soil evaporation and transpiration sub-models was used to scale up the ET measurements to annual values. The data showed large differences in ET depending on site characteristics and on plant attributes. Dense stands of riparian Sandstone Fynbos had an annual ET of 1 460 mm which exceeded the reference ET of 1 346 mm. Dryland Sandstone Fynbos used only 551 mm of water per year while the Sand Plain Fynbos’ annual ET was 1 031 mm, which was similar to the reference ET of 1 059 mm. We conclude that some indigenous plant species use large volumes of water which should be accounted for in, e.g., groundwater recharge estimates, and calculations of incremental water gains after clearing alien invasive plants, among other applications.Keywords: Evapotranspiration, fynbos, scintillometer, Western Cap

The minimum infusion rate of alfaxalone during its co-administration with lidocaine at three different doses by constant rate infusion in goats

OBJECTIVE : To determine the minimum infusion rate (MIR) of alfaxalone required to prevent purposeful movement in response to standardized stimulation while co-administered with lidocaine at three different doses by constant infusion rate infusion (CRI) in goats. STUDY DESIGN : Prospective, blinded, randomized crossover, experimental. ANIMALS : A total of eight healthy goats: four does and four wethers. METHODS: Anaesthetic induction was with lidocaine at 1 mg kg−1 [low dose of lidocaine (L-Lid)], 2 mg kg−1 [moderate dose (M-Lid)] or 4 mg kg−1 [high dose (H-Lid)] and alfaxalone at 2 mg kg−1. Anaesthetic maintenance was with alfaxalone initially at 9.6 mg kg−1 hour−1 combined with one of three lidocaine treatments: 3 mg kg−1 hour−1 (L-Lid), 6 mg kg−1 hour−1 (M-Lid) or 12 mg kg−1 hour−1 (H-Lid). The MIR of alfaxalone was determined by testing for responses to a stimulation in the form of clamping on a digit with a Vulsellum forceps every 30 minutes during lidocaine CRI. Basic cardiopulmonary parameters were measured. RESULTS : The alfaxalone MIRs were 8.64 (6.72–10.56), 6.72 (6.72–8.64) and 6.72 (6.72–6.72) mg kg−1 hour−1 during L-Lid, M-Lid and H-Lid, respectively, without any significant differences among treatments. Compared to the initial rate of 9.6 mg kg−1 hour−1, these reductions in MIR are equivalent to 10, 30 and 30%, respectively. Significant increases in heart rate (HR) and arterial carbon dioxide partial pressure (PaCO2) and decreases in arterial haemoglobin saturation (SaO2), arterial oxygen partial pressure (PaO2) and respiratory frequency (fR) immediately after induction were observed during all lidocaine treatments. CONCLUSIONS AND CLINICAL RELEVANCE : Lidocaine reduces the alfaxalone MIR by up to 30% with a tendency towards a plateauing in this effect at high CRIs. Immediate oxygen supplementation might be required to prevent hypoxaemia.The Beit Trust and National Research Foundation of South Africa.https://www.journals.elsevier.com/veterinary-anaesthesia-and-analgesia2019-05-01hj2018Companion Animal Clinical StudiesSchool of Health Systems and Public Health (SHSPH

Determination of the minimum infusion rate (MIR) of alfaxalone required to prevent purposeful movement of the extremities in response to a standardised noxious stimulus in goats

OBJECTIVE : To determine the minimum infusion rate (MIR) of alfaxalone required to prevent purposeful movement of the extremities in response to noxious stimulation. STUDY DESIGN : Prospective, experimental.Animals Eight healthy goats; four does and four wethers. METHODS : Anaesthesia was induced with alfaxalone 3 mg kg 1 intravenously (IV). A continuous IV infusion of alfaxalone, initially at 0.2 mg kg 1 minute 1, was initiated. Following endotracheal intubation the goats breathed spontaneously via a circle breathing circuit delivering supplementary oxygen.The initial infusion rate was maintained for 30 minutes before testing for responses. The stimulus was clamping on the proximal (soft) part of one digit of the hoof with Vulsellum forceps for 60 seconds. In the absence or presence of purposeful movement of the extremities, the infusion ratewasreduced or increased by 0.02 mg kg 1 minute 1 and held constant for 30 minutes before claw-clamping again. Alfaxalone MIR was calculated as the mean of the infusion rates that allowed and abolished movement. Cardiorespiratory parameters were measured. Recovery from general anaesthesia was timed and quality scored. Results are presented as median (range). RESULTS : The MIR of alfaxalone was 0.16 (0.14– 0.18) mg kg 1 minute 1 or 9.6 (8.4–10.8) mg kg 1 hour 1. Induction of and recovery from anaesthesia were excitement-free. Cardio-respiratory changes were minimal, although compared to baseline HR increased, and at 2 minutes postinduction,(prior to oxygen supplementation), PaO2 decreased significantly from 84 (80–88) to 70 (51– 72) mmHg [11.2 (10.7–11.7) to 9.3 (6.8–9.6) kPa]. Sporadic muscle twitches, unrelated to depth of anaesthesia, were observed during the period of general anaesthesia. Time (minutes) to sternal recumbency and standing were 4.0 (3.0–10.0) and 41.5 (25.0–57.0) respectively. CONCLUSIONS AND CLINICAL RELEVANCE : Alfaxalone can be used for total intravenous anaesthesia (TIVA) in goats and is associated with minimal adverse effects. Oxygen supplementation is advised, especially when working at higher altitudes.Jointly funded by the National Research Foundation (NRF), the Beit Trust and the University of Pretoria.http://onlinelibrary.wiley.comjournal/10.1111/(ISSN)1467-29952016-01-31hb201

Modelling water utilization patterns in apple orchards with varying canopy sizes and different growth stages in semi-arid environments

Accurate estimates of orchard evapotranspiration (ET) and its components are important for precise irrigation scheduling, irrigation system designs, and optimal on-farm water allocation particularly in water-limited environments. Direct measurements of ET remain costly, laborious and sometimes difficult to apply over heterogeneous surfaces such as crop fields. Therefore, accurate crop water-use models are required for on-farm precise water resources management. In this study, we adopted and improved the Priestley-Taylor Jet Propulsion Laboratory (PT-JPL) model developed by Fisher et al 2008 to estimate crop water use across different apple plants. Specifically, the model was developed to quantify the partitioning of apple orchard water use into beneficial (tree transpiration) and non-beneficial water use (orchard floor evaporation) as influenced by tree canopy cover. Data were collected in twelve orchards spread across key apple producing regions in the Western Cape Province of South Africa over three growing seasons (2014/15, 2015/16, 2016/17). Model ET estimates were tested against ET data measured; using the eddy covariance method and transpiration measured based on sap flow monitoring techniques. The results showed that the original Fisher PT-JPL model performed poorly in ET estimation across all the orchards under study

Contribution of understorey vegetation to evapotranspiration partitioning in apple orchards under Mediterranean climatic conditions in South Africa

Orchard evapotranspiration (ET) is a complex flux which has been the subject of many studies. It often includes transpiration from the trees, cover crops and weeds, evaporation from the soil, mulches and other orchard artefacts. In this study we investigated the contribution of the orchard floor evaporative fluxes to whole orchard ET focusing on the transpiration dynamics of understorey vegetation which is currently not well known. Data on the partitioning of ET into its constituent components were collected in apple (Malus Domestica Bork) orchards with varying fractional canopy cover. The study orchards were in the prime apple growing regions in South Africa. The orchards were planted to the Golden Delicious/Reinders and the red cultivars (i.e. Cripps’ Pink/ Royal Gala/Fuji). Tree transpiration was quantified using the heat ratio method and the thermal dissipation sap flow techniques. Understorey transpiration was measured at selected intervals using micro stem heat balance sap flow gauges calibrated against infrared gas analyser readings. Orchard ET was measured using an open path eddy covariance system while the microclimate, radiation interception, and soil evaporation were also monitored. Orchard floor evaporative fluxes accounted for as much as 80% of the measured ET in young orchards with dense understorey vegetation that covered most of the orchard floor

Water use of selected cover crop species commonly grown in South African fruit orchards and their response to drought stress

Cover crops are widely planted in orchards for a variety of reasons. These include suppressing soil erosion, nutrient cycling, phytosanitary purposes, general orchard aesthetics etc. However, there is need to balance these benefits against use of scarce resources such as water and nutrients. Currently no information exists on how different cover crop species use water in orchards and how they cope with drought stress. The aim of this study was therefore to compare the transpiration dynamics of various cover crop types in order to identify species with conservative water use rates. Studied species included: 1) two exotic legumes i.e. Lupine (Lupinus albus L.), and Common vetch (Vicia sativa), 2) three exotic grasses i.e. Tall fescue (Festuca arundinacea), Rye grass (Lolium perenne), and Kikuyu grass (Pennisetum clandestium) and; 3) grasses that are indigenous to sub-Saharan Africa i.e. African Lovegrass (Eragrostis capensis) and Rhodes grass (Chloris gayana). The crops were planted in pots under controlled greenhouse conditions. Transpiration rates were quantified using miniature stem heat balance sap flow gauges and by manual weighing. Drought stress was imposed by withholding irrigation at selected intervals and the responses were quantified through changes in the water relations of the plants. The study showed that exotic legumes had the highest daily water use which peaked at about 2.4 L per square metre of leaf area per day, followed by exotic grasses at 1.5–2.0 L/m2/d. The indigenous grasses used the least water ranging from 0.8 to 1.2 L/m2/d. The indigenous grasses largely displayed an isohydric response to drought stress by maintaining their leaf water status with increasing soil water deficit. The exotic species, on the other hand, showed risk taking behaviour (anisohydry) wherein both the transpiration and leaf water status decreased sharply as drought stress increased. Consequently, some exotic species failed to recover when stress was relieved. From a water use perspective, this study demonstrates that indigenous grass species are more appropriate as cover crops in South African orchards because of their low transpiration rates and the ability to cope with extended periods of water deficit. © 2021 Elsevier Lt

Using sap flow data to assess variations in water use and water status of apple orchards of varying age groups in the Western Cape Province of South Africa

No accurate quantitative information currently exists on how water use of apple (Malus domestica) orchards varies from planting to full-bearing age, leading to poor irrigation and water allocation decision making. This study sought to address this knowledge gap by investigating how the water use and tree water status vary with canopy cover, cultivar, and climatic conditions in 12 orchards growing in prime apple-producing regions in South Africa. The orchards were planted to the Golden Delicious/Golden Delicious Reinders cultivars which are widely planted in South Africa and the Cripps’ Pink/Cripps’ Red/Rosy Glow which are high-value lateseason cultivars. The performance of two transpiration reduction coefficients, one based on sap flow (Ksf) and the other based on soil water depletion (Ks) (FAO approach) were evaluated against the midday stem water potential (MSWP) in all the orchards. While canopy cover had a clear effect on the whole-tree sap flow rates, there were no significant differences in the transpiration per unit leaf area among the cultivars. The daily average sap flux density under unstressed conditions was highest (~284 cm3∙cm–2) in the medium canopy cover orchards (30–44% fractional cover), followed by the mature orchards (~226 cm3∙cm–2), and was lowest in the young orchards (~137 cm3∙cm–2). Canopy cover rather than growing season length had a greater effect on seasonal total water use. Peak daily orchard transpiration ranged from 1.7 mm for young Golden Delicious Reinders trees to 5.0 mm in mature Golden Delicious trees that were maintained with  large canopies to reduce sunburn damage to the fruit. For the red cultivars, the peak daily transpiration ranged from 2.0 to 3.9 mm, and the mature trees were maintained with less dense canopies to facilitate the development of the red fruit colour. The less dense canopies on the red cultivars had water-saving benefits since the seasonal total transpiration was lower relative to the Golden Delicious cultivar. The sap flow derived stress  coefficient was strongly correlated to the MSWP (R2 ~ 0.60–0.97) in all the orchards while Ks was not able to detect plant stress due to over-irrigation. Keywords: cultivar canopy cover transpiration reduction xylem water potentia

Implementation of groundwater protection measures, particularly resource directed measures in South Africa: a review paper

This review paper on groundwater protection measures in South Africa focuses on the actual implementation of groundwater protection measures, in particular, the resource-directed measures (RDM) as described in Chapter 3 of the National Water Act (NWA). Significant catchment-wide implementation of RDM has taken place in a phased manner throughout various catchments since 2012. By 2015, approximately R380 million had been expended on the catchment-wide implementation of the water resource protection measures over a period of 15 years. Considerable effort went into refining the RDM methodology, taking into account the groundwater component of the overall resource. In this paper, we contend that RDM, in its present form, will not make a significant contribution to groundwater resource protection and security in the country. This is a major concern because the Groundwater Strategy of the Department of Human Settlements, Water and Sanitation (DHSWS) had declared the protection of groundwater as a national priority. This paper also examines institutional and governance arrangements (or lack thereof) as well as providing recommendations to support the effective implementation of groundwater protection provisions as prescribed by South Africa’s water legislation

Evaluation of letsoku and related Southern African clayey soils

The nature of letsoku and related clayey soils, traditionally used by indigenous Southern African communities for a wide range of purposes, was explored. Thirty nine samples were collected from Botswana, Lesotho, Swaziland, South Africa and Zimbabwe. They were analyzed to determine their composition and physical properties. Analyses involved BET surface area determinations, pH measurements, X-ray diffraction (XRD), X-ray fluorescence spectroscopy (XRF), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Structured interviews were used to establish the purpose of use and the location of sourcing sites. Most of the samples were in powder form and some were supplied as dry clay balls. Cosmetic applications were almost universally indicated. However, other functions, related to artwork, medicinal use, cultural symbolism and traditional beliefs were also mentioned. The letsoku samples covered a wide range of colors ranging from bright red to yellow but also from off-white to black with some having a light grey color. It was therefore not surprising that the mineral composition of the letsoku samples also varied widely. A black sample, and the yellow and reddish pastel colored samples, contained significant quantities of the corresponding, color imparting, iron oxides. As expected, clay minerals featured prominently although kaolinite was more often encountered than smectites as the dominant minerals. All samples contained silica and in some instances the content exceeded 90% m/m SiO2. The presence of high contents (40% m/m) of gibbsite in samples from Venda represents a new finding for clayey soils in traditional usage.Supplementary Material 1a. Acquired XRD patterns.Supplementary Material 1b. XRD Mineral composition.Tshwane University of Technology (TUT) in the form of a sabbatical stay; TUT DHET RDG funding travel and chemical expenses. Financial support from the Institute of Material Science, University of Pretoria.http://www.elsevier.com/locate/catena2019-12-01hj2018Chemical EngineeringGeologySchool of Health Systems and Public Health (SHSPH

Malnutrition enteropathy in Zambian and Zimbabwean children with severe acute malnutrition: A multi-arm randomized phase II trial.

Malnutrition underlies almost half of all child deaths globally. Severe Acute Malnutrition (SAM) carries unacceptable mortality, particularly if accompanied by infection or medical complications, including enteropathy. We evaluated four interventions for malnutrition enteropathy in a multi-centre phase II multi-arm trial in Zambia and Zimbabwe and completed in 2021. The purpose of this trial was to identify therapies which could be taken forward into phase III trials. Children of either sex were eligible for inclusion if aged 6-59 months and hospitalised with SAM (using WHO definitions: WLZ <-3, and/or MUAC <11.5 cm, and/or bilateral pedal oedema), with written, informed consent from the primary caregiver. We randomised 125 children hospitalised with complicated SAM to 14 days treatment with (i) bovine colostrum (n = 25), (ii) N-acetyl glucosamine (n = 24), (iii) subcutaneous teduglutide (n = 26), (iv) budesonide (n = 25) or (v) standard care only (n = 25). The primary endpoint was a composite of faecal biomarkers (myeloperoxidase, neopterin, α1-antitrypsin). Laboratory assessments, but not treatments, were blinded. Per-protocol analysis used ANCOVA, adjusted for baseline biomarker value, sex, oedema, HIV status, diarrhoea, weight-for-length Z-score, and study site, with pre-specified significance of P < 0.10. Of 143 children screened, 125 were randomised. Teduglutide reduced the primary endpoint of biomarkers of mucosal damage (effect size -0.89 (90% CI: -1.69,-0.10) P = 0.07), while colostrum (-0.58 (-1.4, 0.23) P = 0.24), N-acetyl glucosamine (-0.20 (-1.01, 0.60) P = 0.67), and budesonide (-0.50 (-1.33, 0.33) P = 0.32) had no significant effect. All interventions proved safe. This work suggests that treatment of enteropathy may be beneficial in children with complicated malnutrition. The trial was registered at ClinicalTrials.gov with the identifier NCT03716115