37 research outputs found
Rickettsia and other intracellular bacteria: recent outbreaks, novel pathogens, emerging diseases, new tools, and outstanding discoveries.
Rickettsia and other intracellular bacteria: recent outbreaks, novel pathogens, emerging diseases, new tools, and outstanding discoverie
Serological and molecular survey of Anaplasma species infection in dogs with suspected tickborne disease in Portugal
Fifty-five dogs with suspected tickborne disease were
tested by immunofluorescence assay and PCR for
Anaplasma phagocytophilum infection. Thirty (54·5 per
cent) of the dogs were seropositive and five of them
fulfilled the serological criteria for an active infection,
with either seroconversion or a fourfold increase in
antibody titres. Fragments of DNA of the expected
size were detected by PCR in two seropositive and
three seronegative dogs. However, direct amplicon
sequencing failed to identify active A phagocytophilum
infections, but revealed the presence of Anaplasma
platys DNA in the PCR-positive animals