Rapid determination of trace Cu 2+ by an in-syringe membrane SPE and membrane solid-phase spectral technique

A new in-syringe membrane SPE and solid-phase visible spectral method was proposed for the rapid extraction and visible spectral determination of trace Cu2+. The chelation and membrane SPE can be accomplished in a syringe. The yellow Cu(DDTC)2 complex was separated using a polyethersulfone membrane from the sample solution. Then, the complex can be detected directly on the polyethersulfone membrane utilizing solid-phase visible absorbance spectra without elution. The proposed method simplified the experimental procedure and improved the sensitivity to the μg L-1 level. Furthermore, this method is environmentally friendly since it avoids the use of organic solvents. After the investigation of the influence of different variables on the membrane SPE procedure, water and blood plasma were analyzed to validate the proposed method. A LOD of 0.04 μg L-1 and recoveries of 96.0-103.7% confirmed that the present work can be applied for the determination of trace Cu2+ in water and blood plasma samples

Effect of recombinant human bone morphogenetic protein 2/poly-lactide-co-glycolic acid (rhBMP-2/PLGA) with core decompression on repair of rabbit femoral head necrosis

ABSTRACTObjectiveTo observe the effect of recombinant human bone morphogenetic protein 2/poly-lactide-co-glycolic acid (rhBMP-2/PLGA) with core decompression on repair of rabbit femoral head necrosis.MethodBilateral femoral head necrosis models of rabbit were established by steroid injection. A total of 48 rabbits (96 femoral head necrosis) were randomly divided into 4 groups: Group A, control group with12 rabbits, 24 femoral head necrosis; Group B, treated with rhBMP-2/PLGA implantation after core depression, with 12 rabbits, 24 femoral head necrosis; Group C, treated with rhBMP-2 implantation after core depression, with 12 rabbits, 24 femoral head necrosis; Group D treated with core depression group without implantation, with 12 rabbits, 24 femoral head necrosis. All animals were sacrificed after 12 weeks. The ability of repairing bone defect was evaluated by X-ray radiograph. Bone mineral density analysis of the defect regions were used to evaluate the level of ossification. The morphologic change and bone formation was assessed by HE staining. The angiogenesis was evaluated by VEGF immunohistochemistry.ResultsThe osteogenetic ability and quality of femoral head necrosis in group B were better than those of other groups after 12 weeks by X-ray radiograph and morphologic investigation. And the angiogenesis in group B was better than other groups. Group C had similar osteogenetic quality of femoral head necrosis and angiogenesis with group D.ConclusionThe treatment of rhBMP-2/PLGA implantation after core depression can promote the repair of rabbit femoral head necrosis. It is a promising and efficient synthetic bone material to treat the femoral head necrosis

EVD Surgical Guidance with Retro-Reflective Tool Tracking and Spatial Reconstruction using Head-Mounted Augmented Reality Device

Augmented Reality (AR) has been used to facilitate surgical guidance during External Ventricular Drain (EVD) surgery, reducing the risks of misplacement in manual operations. During this procedure, the pivotal challenge is the accurate estimation of spatial relationship between pre-operative images and actual patient anatomy in AR environment. In this research, we propose a novel framework utilizing Time of Flight (ToF) depth sensors integrated in commercially available AR Head Mounted Devices (HMD) for precise EVD surgical guidance. As previous studies have proven depth errors for ToF sensors, we first conducted a comprehensive assessment for the properties of this error on AR-HMDs. Subsequently, a depth error model and patient-specific model parameter identification method, is introduced for accurate surface information. After that, a tracking procedure combining retro-reflective markers and point clouds is proposed for accurate head tracking, where head surface is reconstructed using ToF sensor data for spatial registration, avoiding fixing tracking targets rigidly on the patient's cranium. Firstly, $7.580\pm 1.488 mm$ ToF sensor depth value error was revealed on human skin, indicating the significance of depth correction. Our results showed that the ToF sensor depth error was reduced by over $85\%$ using proposed depth correction method on head phantoms in different materials. Meanwhile, the head surface reconstructed with corrected depth data achieved sub-millimeter accuracy. Experiment on a sheep head revealed $0.79 mm$ reconstruction error. Furthermore, a user study was conducted for the performance of proposed framework in simulated EVD surgery, where 5 surgeons performed 9 k-wire injections on a head phantom with virtual guidance. Results of this study revealed $2.09 \pm 0.16 mm$ translational accuracy and $2.97\pm 0.91 ^\circ$ orientational accuracy

TMRT observations of 26 pulsars at 8.6 GHz

Integrated pulse profiles at 8.6~GHz obtained with the Shanghai Tian Ma Radio Telescope (TMRT) are presented for a sample of 26 pulsars. Mean flux densities and pulse width parameters of these pulsars are estimated. For eleven pulsars these are the first high-frequency observations and for a further four, our observations have a better signal-to-noise ratio than previous observations. For one (PSR J0742-2822) the 8.6~GHz profiles differs from previously observed profiles. A comparison of 19 profiles with those at other frequencies shows that in nine cases the separation between the outmost leading and trailing components decreases with frequency, roughly in agreement with radius-to-frequency mapping, whereas in the other ten the separation is nearly constant. Different spectral indices of profile components lead to the variation of integrated pulse profile shapes with frequency. In seven pulsars with multi-component profiles, the spectral indices of the central components are steeper than those of the outer components. For the 12 pulsars with multi-component profiles in the high-frequency sample, we estimate the core width using gaussian fitting and discuss the width-period relationship.Comment: 33 pages, 49 figures, 5 Tables; accepted by Ap

Mixed Diets Reduce the Oxidative Stress of Common Carp (Cyprinus carpio): Based on MicroRNA Sequencing

The rice-fish mode, a mode of ecological aquaculture, has become a popular research topic in recent years. The antioxidant capacity of fish can be affected by the type of diet. Three groups of adult common carp (initial weight 517.8 ± 50 g) were fed earthworm (group A), earthworm + duckweed (group M), and duckweed (group P). The antioxidant capacity of common carp (Cyprinus carpio) was evaluated by histopathological sectioning, antioxidant enzyme activity, and the miRNA transcriptome profile. The pathological changes in group M were lighter than those in groups C and A. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) significantly increased in group M, and the malondialdehyde content (MDA) significantly decreased (p < 0.05). Additionally, nine differentially expressed miRNAs (DEMs) were found between groups A and M, and eight DEMs found between groups P and M were identified in the liver of common carp. Five miRNAs were reported to be related to oxidative stress, including miR-137-3p, miR-143-3p, miR-146a-5p, miR-21-5p, and miR-125b-5p. Compared with group M, all five detected miRNAs were upregulated in group A, and four of the detected miRNAs were upregulated in group P. The targets of the five miRNAs were further predicted via functional analysis. Our study confirmed that omnivorous common carp exhibits stronger antioxidant capacity when feeding on both an animal diet and a plant diet

Research of fermentation preparation technology and preliminary application for Deoxynivalenol-degrading direct inoculated microbial inoculum

Deoxynivalenol(DON)，as a secondary metabolite of some fusarium species，has been harmful to the food and feed industry.To improve spore-producing ability of Bacillus subtilis，the fermentation pH，temperature，supplemental carbon source and duration of Bacillus subtilis that efficiently degraded DON were studied in 5L-quadruple fermenters.The best fermentation conditions were determined，the fermentation pH was controlled at 7.5 and the temperature was controlled by stages，kept at 37 ℃ for first 12 hours，then raised to 39 ℃ until the end; 10 g/L molasses was added once at the 12th hour，then the fermentation was terminated at the 30th hour.Under the optimal conditions，the number of spores could reach 2.9×1010 CFU/mL and the spores’ rate could reach 96.7%.The fermentation liquid were further prepared into direct inoculated microbial inoculum and applied to remove DON from grain processing by-products; Under the condition that dry matter content in the composite material culture medium was not more than 30%，and the inoculation amount of the bacterium preparation was more than 0.1%，DON in the grain by-products could be effectively removed by fermentation and the highest degradation rate could reach 98.8%

Efficient W state entanglement concentration using quantum-dot and optical microcavities

We present an entanglement concentration protocols (ECPs) for less-entangled W state with quantum-dot and microcavity coupled system. The present protocol uses the quantum nondemolition measurement on the spin parity to construct the parity check gate. Different from other ECPs, this less-entangled W state with quantum-dot and microcavity coupled system can be concentrated with the help of some single photons. The whole protocol can be repeated to get a higher success probability. It may be useful in current quantum information processing.Comment: 9 pages, 6 figure