From Material Collection to Ideological Presentation: A Paradigm Shift in Museum Practice

Under the impetus of the New Museum Movement, the definition of museum has been updated. Museums are no longer merely places of “collection, storage and exhibition”, but also have functions of “research, education and pleasure”. Although “comprehensive and scarce” collection is important, it is no longer the only standard for museum exhibition. At the same time, new ideas have emerged that emphasize “human” subjectivity, multiple expressions, and situational narratives. As a result, the paradigm shift from material collection to ideological presentation has become a specific direction for museum practice in China today

Investigation and Analysis of Present Situation and Educational Countermeasures and Cultural Needs of Farmers

The samples, from 21 provinces, municipalities directly under the central government, 107 villages, 30 towns, are altogether 3,577. The survey found, the cultural demand of farmers is generally healthy and positive. What they need most are cultural knowledge of computer technology, disease prevention knowledge, legal knowledge to deal with disputes, migrant workers rights of women and children protection law, recreational activities are singing and dancing, favorite sports activities the first three are the small ball (table tennis badminton), major sports (basketball and volleyball football) and the short or long runs. And there are some differences in age, gender, and cultural degree of life state between these demands. Investigation and analysis reflect that, some activities already undertaken can’t meet the need, and some farmers on the surface needn’t culture, but actually reflect the lag of cultural spiritual pursuit. By contrast with sports, entertainment is more short of physical activity. Farmers’ favorite access to cultural knowledge is face-to-face teaching. However, the “information price ratio” of “three rural areas “ activities are lower than others. According to the problems, we put forward some strategic suggestions on software and hardware

Mechanical characterization of therapeutic cells and physical property-based sorting in microfluidic systems

Bone marrow-derived mesenchymal and hematopoietic stem cells (MSCs and HSCs) have rapidly become the leading cells for consideration to aid tissue regeneration following injury. However, potential HSCs and MSCs migration to the injured tissue after infusion is impeded by cell trap within the upstream vessels, where physical and mechanical properties of cells play an important role. Microfluidic system has a potential to sort cells/particles based on their mechanical properties. It is hypothesized that such sorting system could be utilized to separate smaller and more deformable SCs from a cell population, infusion of which might be able to enhance the recruitment of the cells. The mechanical properties of murine HSCs were determined using micromanipulation and atomic force microscopy (AFM). Microfluidic devices were fabricated to separate sub-set of HSCs, followed by the infusion of the isolated cells into ischemia-reperfusion injured animals. HSCs as a whole became weaker and more deformable after pre-treatment with SDF-la and H 2 02, but HSC surface stiffened after the same pre-treating, accompanied by the expansion and polymerization ofF-actin interacting with the plasma membrane. A spiral microfluidic system with channel width 300m and height 40m was found to effectively isolate smaller and more deformable HSCs from a cell population, resulting in a significant increase of free flowing cells in vivo. This study comprehensively characterized cell mechanics at different levels using micromanipulation and AFM, determining mechanical markers of therapeutic cells. Most importantly, a simple cell sorting system was successfully developed to isolate target cells without introducing any chemical modification, and the possible underlying mechanism was discussed, which can be valuable to cellular therapy

Mechanical characterization of therapeutic cells and physical property-based sorting in microfluidic systems

Bone marrow-derived mesenchymal and hematopoietic stem cells (MSCs and HSCs) have rapidly become the leading cells for consideration to aid tissue regeneration following injury. However, potential HSCs and MSCs migration to the injured tissue after infusion is impeded by cell trap within the upstream vessels, where physical and mechanical properties of cells play an important role. Microfluidic system has a potential to sort cells/particles based on their mechanical properties. It is hypothesized that such sorting system could be utilized to separate smaller and more deformable SCs from a cell population, infusion of which might be able to enhance the recruitment of the cells. The mechanical properties of murine HSCs were determined using micromanipulation and atomic force microscopy (AFM). Microfluidic devices were fabricated to separate sub-set of HSCs, followed by the infusion of the isolated cells into ischemia-reperfusion injured animals. HSCs as a whole became weaker and more deformable after pre-treatment with SDF-la and H 2 02, but HSC surface stiffened after the same pre-treating, accompanied by the expansion and polymerization ofF-actin interacting with the plasma membrane. A spiral microfluidic system with channel width 300m and height 40m was found to effectively isolate smaller and more deformable HSCs from a cell population, resulting in a significant increase of free flowing cells in vivo. This study comprehensively characterized cell mechanics at different levels using micromanipulation and AFM, determining mechanical markers of therapeutic cells. Most importantly, a simple cell sorting system was successfully developed to isolate target cells without introducing any chemical modification, and the possible underlying mechanism was discussed, which can be valuable to cellular therapy