Cyclooxygenase Inhibition Limits Blood-Brain Barrier Disruption following Intracerebral Injection of Tumor Necrosis Factor-alpha in the Rat

Increased permeability of the blood-brain barrier (BBB) is important in neurological disorders. Neuroinflammation is associated with increased BBB breakdown and brain injury. Tumor necrosis factor-alpha (TNF-a) is involved in BBB injury and edema formation through a mechanism involving matrix metalloproteinase (MMP) upregulation. There is emerging evidence indicating that cyclooxygenase (COX) inhibition limits BBB disruption following ischemic stroke and bacterial meningitis, but the mechanisms involved are not known. We used intracerebral injection of TNF-a to study the effect of COX inhibition on TNF-a-induced BBB breakdown, MMP expression/activity and oxidative stress. BBB disruption was evaluated by the uptake of 14C-sucrose into the brain and by magnetic resonance imaging (MRI) utilizing Gd-DTPA as a paramagnetic contrast agent. Using selective inhibitors of each COX isoform, we found that COX-1 activity is more important than COX-2 in BBB opening. TNF-a induced a significant upregulation of gelatinase B (MMP-9), stromelysin-1 (MMP-3) and COX-2. In addition, TNF-a significantly depleted glutathione as compared to saline. Indomethacin (10 mg/kg; i.p.), an inhibitor of COX-1 and COX-2, reduced BBB damage at 24 h. Indomethacin significantly attenuated MMP-9 and MMP-3 expression and activation, and prevented the loss of endogenous radical scavenging capacity following intracerebral injection of TNF-a. Our results show for the first time that BBB disruption during neuroinflammation can be significantly reduced by administration of COX inhibitors. Modulation of COX in brain injury by COX inhibitors or agents modulating prostaglandin E2 formation/signaling may be useful in clinical settings associated with BBB disruption

The Forward Testing Effect: Interim Testing Enhances Inductive Learning.

Induction refers to the process in which people generalize their previous experience when making uncertain inferences about the environment that go beyond direct experience. Here we show that interim tests strongly enhance inductive learning. Participants studied the painting styles of eight famous artists across four lists, each comprising paintings by one pair of artists. In an interim test group participants’ induction was tested after each list. In two control groups participants solved math problems (interim math group) or studied additional new paintings (interim study group) following each of Lists 1–3 and were asked to classify new paintings on List 4. In the List 4 interim test, the interim test group significantly outperformed the other two groups, indicating that interim testing enhances new inductive learning. In a final cumulative test, accuracy in the interim test group at classifying new paintings by studied artists was nearly double that of the other two groups, indicating the major importance of interim testing in inductive learning. This enhancing effect of interim testing on inductive learning was associated with metacognitive awareness

The feedback triangle and the enhancement of dialogic feedback processes

This article explores some of the main barriers to the enhancement of feedback processes and proposes a framework for using dialogic feedback to foster productive student learning in the discipline. The framework suggests a feedback triangle focused on the content of feedback (cognitive dimension), the interpersonal negotiation of feedback (social-affective dimension) and the organisation of feedback provision (structural dimension). The interplay between these three elements is central to prospects for the enhancement of feedback processes. Derived from the framework is a set of six key features of optimal feedback practice which we represent as building blocks of an architecture of dialogic feedback. The paper concludes with a research agenda which suggests issues to be further explored in the cognitive, social-affective and structural dimensions.postprin

Modular 3D Interface Design for Accessible VR Applications

Designed with an accessible first design approach, the presented paper describes how exploiting humans proprioception ability in 3D space can result in a more natural interaction experience when using a 3D graphical user interface in a virtual environment. The modularity of the designed interface empowers the user to decide where they want to place interface elements in 3D space allowing for a highly customizable experience, both in the context of the player and the virtual space. Drawing inspiration from todays tangible interfaces used, such as those in aircraft cockpits, a modular interface is presented taking advantage of our natural understanding of interacting with 3D objects and exploiting capabilities that otherwise have not been used in 2D interaction. Additionally, the designed interface supports multimodal input mechanisms which also demonstrates the opportunity for the design to cross over to augmented reality applications. A focus group study was completed to better understand the usability and constraints of the designed 3D GUI.Comment: This preprint has not undergone peer review or any post-submission corrections. The Version of Record of this contribution will be published in Springer Nature Computer Science book series in Volume HCI International 202

Comparative Verification of Staphylococcal Related Enterotoxin in Staphylococcus aureus Study Samples

Staphylococcal related diseases such as food poisoning and toxic shock syndrome are often associated by specific enterotoxin produced by Staphylococcus aureus. Enterotoxin can disrupt the body\u27s immune system response against pathogens and indirectly damage tissues and organs as a result. To identify the specific toxins produced by S.aureus, the gene encoding for the toxin in question was expressed though the use of its respective primers and a polymerase chain reaction

ASSESSING THE MENTAL STRESS OF HEALTHCARE WORKERS DURING THE COVID-19 PANDEMIC: A COMPARATIVE STUDY

Abstract The COVID-19 pandemic has placed significant psychological burdens on healthcare workers, leading to increased mental stress and burnout. This research paper aims to assess the mental stress experienced by healthcare workers during the pandemic and compare it across different healthcare settings and professional roles. The study will employ a cross-sectional design, targeting healthcare workers from various healthcare settings, including hospitals, clinics, and long-term care facilities. A standardized questionnaire, such as the Perceived Stress Scale (PSS), will be used to assess the levels of mental stress among participants. The survey will also collect demographic information, work-related factors, such as work hours and direct patient contact, and coping strategies employed by healthcare workers to mitigate stress. Data analysis will involve descriptive statistics to determine the overall levels of mental stress. Furthermore, the stress levels will be compared across different healthcare settings and professional roles using appropriate statistical tests. The findings will provide insights into the mental stress experienced by healthcare workers during the pandemic and shed light on the factors contributing to heightened stress levels. This information can be utilized to develop targeted interventions, support systems, and mental health programs to address the mental well-being of healthcare workers