17 research outputs found
Some virulence genes of Escherichia coli isolated from cloacal swabs of healthy Alagoas Curassows (Pauxi mitu) in Brazil
Expression of P, S, and F1C adhesins by cytotoxic necrotizing factor1-producing Escherichia coli from septicemic and diarrheic pigs
International audienc
PCR na detecção de gene Fel A de Escherichia Coli em frangos de corte condenados por aerossaculite pela Inspeção Sanitåria Federal
Distribution of virulence genes and multiple drugâresistant patterns amongst different phylogenetic groups of uropathogenic Escherichia coli
Cloning, chromosomal location, and tissue expression of the gene for pig interleukin-18
Chantier qualité spécifique "Auteurs Externes" département de Génétique animale : uniquement liaison auteur au référentiel HR-AccessInternational audienc
A reverse transcription-polymerase chain reaction method to analyze porcine cytokine gene expression
International audienc
L'interleukine-1 porcine: caractérisation et expression au niveau intestinal
National audienc
Avian Extraintestinal Escherichia Coli Exhibits Enterotoxigenic-like Activity In The In Vivo Rabbit Ligated Ileal Loop Assay
Avian pathogenic Escherichia coli (APEC) strains harbor a number of virulence genes and cause extraintestinal diseases, such as septicemia, swollen-head syndrome, salpingitis, and omphalitis in poultry. APEC strains are not known to cause intestinal diseases. Herein, for the first time, it is reported that APEC strains were able to induce an enterotoxigenic-like effect in rabbit ligated ileal loops. Strain SEPT362 caused cell detachment of the intestinal villi, which also showed a flattened and wilted appearance, but the integrity of the tight junctions was maintained. Additionally, this strain did not adhere to enterocytes in vivo, although adhesin encoding genes (fimH, csgA, lpfA2-3, and ECP) were present while other lpfA types, sfa, afa, papC, and ral genes were not. This enterotoxigenic-like activity was conserved after thermal treatment of the supernatant at 65°C but not at 100°C. Moreover, experiments based on filtering with different molecular weight cut-off (MWCO) pore sizes demonstrated that the component associated with the observed biological effect has a molecular weight >100 kDa. Blast search and polymerase chain reaction assays for known E. coli virulence factors showed that strain SEPT362 harbors the gene encoding for the toxin EAST-1 and the serine protease autotransporter (SPATE) Tsh, but is negative for genes encoding for the toxins LT-I, STh, STp, Stx1, Stx2, CNF-1, CNF-2, CDT and the SPATEs Sat, Pic, Vat, SigA, SepA, EatA, EspP, or EspC. A cloned copy of the tsh gene in E. coli K-12 was also tested and was shown to have an enterotoxic effect. These results suggest that APEC might induce fluid accumulation in the rabbit gut. The Tsh autotransporter seems to be one of the factors associated with this phenotype. © Copyright 2014, Mary Ann Liebert, Inc. 2014.116484489Adlerberth, I., Hanson, L., Svanborg, C., Svennerholm, A., Nordgren, S., Wold, A., Adhesins of Escherichia coli associated with extra-intestinal pathogenicity confer binding to colonic epithelial cells (1995) Microb Pathog, 18, pp. 373-385Bauchart, P., Germon, P., Brée, A., Oswald, E., Hacker, J., Dobrindt, U., Pathogenomic comparison of human extraintestinal and avian pathogenic Escherichia coli-Search for factors involved in host specificity or zoonotic potential (2010) Microb Pa-thog, 49, pp. 105-115Bélanger, L., Garenaux, A., Harel, J., Boulianne, M., Nadeau, E., Dozois, C.M., Escherichia coli from animal reservoirs as a potential source of human extraintestinal pathogenic E coli (2011) FEMS Immunol Med Microbiol, 62, pp. 1-10Blanco, J.E., Blanco, M., Mora, A., Blanco, J., Production of toxins (enterotoxins, verotoxins, and necrotoxins) and colicins by Escherichia coli strains isolated from septicemic and healthy chickens: Relationship with in vivo pathogenicity (1997) J Clin Microbiol, 35, pp. 2953-2957Borges, C.A., Beraldo, L.G., Maluta, R.P., Shiga toxigenic and atypical enteropathogenic Escherichia coli in the feces and carcasses of slaughtered pigs (2012) Foodborne Pathog Dis, 9, pp. 1119-1125De Pace, F., Boldrin De Paiva, J., Nakazato, G., Characterization of IcmF of the type VI secretion system in an avian pathogenic Escherichia coli (APEC) strain (2011) Microbiology, 157, pp. 2954-2962Dziva, F., Stevens, M., Colibacillosis in poultry: Unravelling the molecular basis of virulence of avian pathogenic Escherichia coli in their natural hosts (2008) Avian Pathol, 37, pp. 355-366Matsuda, K., Chaudhari, A.A., Lee, J.H., Avian colibacillosis caused by an intestinal pathogenic Escherichia coli isolate from calf diarrhea (2010) Res Vet Sci, 89, pp. 150-152Mellata, M., Human and avian extraintestinal pathogenic Es-cherichia coli: Infections, zoonotic risks, and antibiotic resistance trends (2013) Foodborne Pathog Dis, 10, pp. 916-932Ménard, L.-P., Lussier, J.G., Lépine, F., De Sousa, C.P., Dubreuil, J.D., Expression, purification, and biochemical characterization of enteroaggregative Escherichia coli heat-stable enterotoxin 1 (2004) Protein Expr Purif, 33, pp. 223-231Moulin-Schouleur, M., Répérant, M., Laurent, S., Extra-intestinal pathogenic Escherichia coli strains of avian and human origin: Link between phylogenetic relationships and common virulence patterns (2007) J Clin Microbiol, 45, pp. 3366-3376Navarro-Garcia, F., Gutierrez-Jimenez, J., Garcia-Tovar, C., Castro, L.A., Salazar-Gonzalez, H., Cordova, V., Pic, an autotransporter protein secreted by different pathogens in the En-terobacteriaceae family, is a potent mucus secretagogue (2010) Infect Immun, 78, pp. 4101-4109Olesen, B., Scheutz, F., Andersen, R.L., Enteroaggregative Escherichia coli O78:H10 the cause of an outbreak of urinary tract infection (2012) J Clin Microbiol, 50, pp. 3703-3711Provence, D.L., Curtiss, R., Isolation and characterization of a gene involved in hemagglutination by an avian pathogenic Es-cherichia coli strain (1994) Infect Immun, 62, pp. 1369-1380Tcg, R., Maluta, R.P., Parizzi, L.P., Genome sequences of avian pathogenic Escherichia coli strains isolated from Brazilian commercial poultry (2013) Genome Announce, 1, pp. e00110-e00113Russo, T.A., Johnson, J.R., Proposal for a new inclusive designation for extraintestinal pathogenic isolates of Escherichia coli: ExPEC (2000) J Infect Dis, 181, pp. 1753-1754Savarino, S.J., Fasano, A., Robertson, D.C., Levine, M.M., En-teroaggregative Escherichia coli elaborate a heat-stable en-terotoxin demonstrable in an in vitro rabbit intestinal model (1991) J Clin Invest, 87, pp. 1450-1455Schierack, P., Walk, N., Ewers, C., ExPEC-typical virulence-associated genes correlate with successful colonization by intestinal E coli in a small piglet group (2008) Environ Microbiol, 10, pp. 1742-1751Syngkon, A., Elluri, S., Koley, H., Studies on a novel serine protease of a DhapADprtV Vibrio cholerae O1 strain and its role in hemorrhagic response in the rabbit ileal loop model (2010) PLoS ONE, 5. , pii:e13122Mam, V., Tat, G., Ajp, F., Knöbl, T., Servin, A.L., Liévin-Le Moal, V., Two atypical enteropathogenic Escher-ichia coli strains induce the production of secreted and membrane-bound mucins to benefit their own growth at the apical surface of human mucin-secreting intestinal HT29-MTX cells (2010) Infect Immun, 78, pp. 927-93